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LEO1 (NM_138792) Human Untagged Clone
CAT＃: SC120657
LEO1 (untagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1)
CNY 4,888.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RDL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120657 sequence for NM_138792 edited (data generated by NextGen Sequencing)
ATGGCGGATATGGAGGATCTCTTCGGGAGCGACGCCGACAGCGAAGCTGAGCGTAAAGAT TCTGATTCTGGATCTGACTCAGATTCTGATCAAGAGAATGCTGCCTCTGGCAGTAATGCC TCTGGAAGTGAAAGTGATCAGGATGAAAGAGGTGATTCAGGACAACCAAGTAATAAGGAA CTGTTTGGAGATGACAGTGAGGACGAGGGAGCTTCACATCATAGTGGTAGTGATAATCAC TCTGAAAGATCAGACAATAGATCAGAAGCTTCTGAGCGTTCTGACCATGAGGACAATGAC CCCTCAGATGTAGATCAGCACAGTGGATCAGAAGCCCCTAATGATGATGAAGACGAAGGT CATAGATCGGATGGAGGGAGCCATCATTCAGAAGCAGAAGGTTCTGAAAAAGCACATTCA GATGATGAAAAATGGGGCAGAGAAGATAAAAGTGACCAGTCAGATGATGAAAAGATACAA AATTCTGATGATGAGGAGAGGGCACAAGGATCTGATGAAGATAAGCTGCAGAATTCTGAC GATGATGAGAAAATGCAGAACACAGATGATGAGGAGAGGCCTCAGCTTTCCGATGATGAG AGACAACAGCTATCTGAGGAGGAAAAGGCTAATTCTGATGATGAACGGCCGGTAGCTTCT GATAATGATGATGAGAAACAGAATTCTGATGATGAAGAACAACCACAGCTGTCTGATGAA GAGAAAATGCAAAATTCTGATGATGAAAGGCCACAGGCCTCAGATGAAGAACACAGGCAT TCAGATGATGAAGAGGAACAGGATCATAAATCAGAATCTGCAAGAGGCAGTGATAGTGAA GATGAAGTTTTACGAATGAAACGCAAGAATGCGATTGCATCTGATTCAGAAGCGGATAGT GACACTGAGGTGCCAAAAGATAATAGTGGAACCATGGATTTATTTGGAGGTGCAGATGAT ATCTCTTCAGGGAGTGATGGAGAAGACAAACCACCTACTCCAGGACAGCCTGTTGATGAA AATGGATTGCCTCAGGATCAACAGGAAGAGGAGCCAATTCCTGAGACCAGAATAGAAGTA GAAATACCCAAAGTAAACACTGATTTAGGAAACGACTTATATTTTGTTAAACTGCCCAAC TTTCTCAGTGTAGAGCCCAGACCTTTTGATCCTCAGTATTATGAAGATGAATTTGAAGAT GAAGAAATGCTGGATGAAGAAGGTAGAACCAGGTTAAAATTAAAGGTAGAAAATACTATA AGATGGAGGATACGCCGAGATGAAGAAGGAAATGAAATTAAAGAAAGCAATGCTCGGATA GTCAAGTGGTCAGATGGAAGCATGTCCCTGCATTTAGGCAATGAAGTGTTTGATGTGTAC AAAGCCCCACTGCAGGGCGACCACAATCATCTTTTTATAAGACAAGGTACTGGTCTACAG GGACAAGCAGTCTTTAAAACGAAACTCACCTTCAGACCTCACTCTACGGACAGTGCCACA CATAGAAAGATGACTCTGTCACTTGCAGATAGGTGTTCAAAGACACAGAAGATTAGAATC TTGCCAATGGCTGGTCGTGATCCTGAATGCCAACGCACAGAAATGATTAAGAAAGAAGAA GAACGTTTGAGGGCTTCCATACGTAGGGAATCTCAGCAGCGCCGAATGAGAGAGAAACAG CACCAGCGGGGGCTGAGCGCCAGTTACCTGGAACCTGATCGATACGATGAGGAGGAGGAA GGCGAGGAGTCCATCAGCTTGGCTGCCATTAAAAACCGATATAAAGGGGGCATTCGAGAG GAACGAGCCAGAATCTATTCATCAGACAGTGATGAGGGATCAGAAGAAGATAAAGCTCAA AGATTACTCAAAGCAAAGAAACTTACCAGTGATGAGGAAGGTGAACCTTCCGGAAAGAGA AAAGCAGAAGATGATGATAAAGCAAATAAAAAGCATAAGAAGTATGTGATCAGCGATGAA GAGGAAGAAGATGATGATTGA Clone variation with respect to NM_138792.2 >OriGene 5' read for NM_138792 unedited
TAGGGCGGCCCGCGAATTCGCACGAGGCGTAAAGAGAGCCGGGAGCTGCCCCTAACCGAG GCAGCAGCGGACGTGAGCGATAATGGCGGATATGGAGGATCTCTTCGGGAGCGACGCCGA CAGCGAAGCTGAGCGTAAAGATTCTGATTCTGGATCTGACTCAGATTCTGATCAAGAGAA TGCTGCCTCTGGCAGTAATGCCTCTGGAAGTGAAAGTGATCAGGATGAAAGAGGTGATTC AGGACAACCAAGTAATAAGGAACTGTTTGGAGATGACAGTGAGGACGAGGGAGCTTCACA TCATAGTGGTAGTGATAATCACTCTGAAAGATCAGACAATAGATCAGAAGCTTCTGAGCG TTCTGACCATGAGGACAATGACCCCTCAGATGTAGATCAGCACAGTGGATCAGAAGCCCC TAATGATGATGAAGACGAAGGTCATAGATCGGATGGAGGGAGCCATCATTCAGAAGCAGA AGGTTCTGAAAAAGCACATTCAGATGATGAAAAATGGGGCAGAGAAGATAAAAGTGACCA GTCAGATGATGAAAAGATACAAAATTCTGATGATGAGGAGAGGGCACAAGGATCTGATGA AGATAAGCTGCAGAATTCTGACGATGATGAGAAAATGCAGAACACAGATGATGAGGAGAG GCCTCAGCTNTCCGATGATGAGAGACAACAGCTATCTGAGGAAGAAAAGGCTAATTCTGA TGATGAACGGGCCGGNTAGCTTCTGATATGATGATGAGAAACAGAATTCTGATGATGAAG AAACACCACAGCTGTCTGATGAAGAGAAAATGCANNATTCTGATGATGAAAGGCCACACA GCCTCAGATGAAGAACAACAGGCTTTCAGATGATGAAGAGGGAACAGGGATCATAATCAG AATCTGCNAGAA >OriGene 3' read for NM_138792 unedited
NNAAAATTAGGGGTTNAATTGCTTTTTACATTAAAATACACAAAAGCAAATCGATTCATT TCAATCAAAATAACTCATGTTTACATATTTATAACTGTACAATAAAATATATAAAATGTT TTCATATTTCATACTTCAATCATCATCTTCTTCCTCTTCATCGCTGATCACATACTTCTT ATGCTTTTTATTTGCTTTATCATCATCTTCTGCTTTTCTCTTTCCGGAAGGTTCACCTTC CTGTTTTGTCTAAGAAAGCTGAGGGTTGGCCTTGATACCTCTCCTTTCCACTGTCTTCAT GTTTCTTTCAGCTTGGGTCTTTCCCTTTTCCCAGACTCCTCATCACTGGTAAGTTTCTTT GCTTTGAGTAATCTTTGAGCTTTATCTTCTTCTGATCCCTCATCACTGTCTGATGAATAG ATTCTGGCTCGTTCCTCTCGAATGCCCCCTTTATATCGGTTTTTAATGGCAGCCAAGCTG ATGGACTCCTCGCCTTCCTCCTCCTCATCGTATCGATCAGGTTCCAGGTAACTGGCGCTC AGCCCCCGCTGGTGCTGTTTCTCTCTCATTCGGCGCTGCTGAGATTCCCTACGTATGGAA GCCCTCAAACGTTCTTCTTCTTTCTTAATCATTTCTGTGCGTTGGCATTCAGGATCACGA CCAGCCATTGGCAAGATTCTAATCTTCTGTGTCTTTGAACACCTATCTGCAAGTGACAGA GTCATCTTTCTATGTGTGGCACTGGCCGTAGAGTGAAGTCTGAAAGTGAGTTTCGTTTTA AAAACTGCTTGTCCCTGTAGCCAGTACCTTGTCTTTTAAAAAGATAATTGTGGTCCCCCC TGCATGGGGCTTTTGACCCATAAAACCCTTCATTGCCTAAAGGCCGGGCCTTGCCTCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_138792 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138792.2, NP_620147.1 |
| RefSeq Size | 2162 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 123169 |
| UniProt ID | Q8WVC0 |
| Domains | Leo1 |
| Gene Summary | LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Parafibromin Tumor Suppressor Protein Is Part of a Human Paf1 Complex
,Orit Rozenblatt-Rosen, Christina M. Hughes, Suraj J. Nannepaga, Kalai Selvi Shanmugam, Terry D. Copeland, Tad Guszczynski, James H. Resau, and Matthew Meyerson,
Mol. Cell. Biol. 2005 Jan;25(2):612-20.
[LEO1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202274 | LEO1 (Myc-DDK-tagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) |
CNY 4,960.00 |
|
| RC202274L1 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged |
CNY 7,360.00 |
|
| RC202274L2 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged |
CNY 5,890.00 |
|
| RC202274L3 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202274L4 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged |
CNY 5,890.00 |
|
| RG202274 | LEO1 (tGFP-tagged) - Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) |
CNY 6,560.00 |
