LEO1 (NM_138792) Human Tagged ORF Clone

CAT＃: RG202274

TrueORF^®

LEO1 (tGFP-tagged) - Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_138792" in other vectors (6)

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CNY 4,370.00

货期*

现货

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA377993

LEO1 Rabbit polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	RDL
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG202274 representing NM_138792 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATATGGAGGATCTCTTCGGGAGCGACGCCGACAGCGAAGCTGAGCGTAAAGATTCTGATTCTG GATCTGACTCAGATTCTGATCAAGAGAATGCTGCCTCTGGCAGTAATGCCTCTGGAAGTGAAAGTGATCA GGATGAAAGAGGTGATTCAGGACAACCAAGTAATAAGGAACTGTTTGGAGATGACAGTGAGGACGAGGGA GCTTCACATCATAGTGGTAGTGATAATCACTCTGAAAGATCAGACAATAGATCAGAAGCTTCTGAGCGTT CTGACCATGAGGACAATGACCCCTCAGATGTAGATCAGCACAGTGGATCAGAAGCCCCTAATGATGATGA AGACGAAGGTCATAGATCGGATGGAGGGAGCCATCATTCAGAAGCAGAAGGTTCTGAAAAAGCACATTCA GATGATGAAAAATGGGGCAGAGAAGATAAAAGTGACCAGTCAGATGATGAAAAGATACAAAATTCTGATG ATGAGGAGAGGGCACAAGGATCTGATGAAGATAAGCTGCAGAATTCTGACGATGATGAGAAAATGCAGAA CACAGATGATGAGGAGAGGCCTCAGCTTTCCGATGATGAGAGACAACAGCTATCTGAGGAGGAAAAGGCT AATTCTGATGATGAACGGCCGGTAGCTTCTGATAATGATGATGAGAAACAGAATTCTGATGATGAAGAAC AACCACAGCTGTCTGATGAAGAGAAAATGCAAAATTCTGATGATGAAAGGCCACAGGCCTCAGATGAAGA ACACAGGCATTCAGATGATGAAGAGGAACAGGATCATAAATCAGAATCTGCAAGAGGCAGTGATAGTGAA GATGAAGTTTTACGAATGAAACGCAAGAATGCGATTGCATCTGATTCAGAAGCGGATAGTGACACTGAGG TGCCAAAAGATAATAGTGGAACCATGGATTTATTTGGAGGTGCAGATGATATCTCTTCAGGGAGTGATGG AGAAGACAAACCACCTACTCCAGGACAGCCTGTTGATGAAAATGGATTGCCTCAGGATCAACAGGAAGAG GAGCCAATTCCTGAGACCAGAATAGAAGTAGAAATACCCAAAGTAAACACTGATTTAGGAAACGACTTAT ATTTTGTTAAACTGCCCAACTTTCTCAGTGTAGAGCCCAGACCTTTTGATCCTCAGTATTATGAAGATGA ATTTGAAGATGAAGAAATGCTGGATGAAGAAGGTAGAACCAGGTTAAAATTAAAGGTAGAAAATACTATA AGATGGAGGATACGCCGAGATGAAGAAGGAAATGAAATTAAAGAAAGCAATGCTCGGATAGTCAAGTGGT CAGATGGAAGCATGTCCCTGCATTTAGGCAATGAAGTGTTTGATGTGTACAAAGCCCCACTGCAGGGCGA CCACAATCATCTTTTTATAAGACAAGGTACTGGTCTACAGGGACAAGCAGTCTTTAAAACGAAACTCACC TTCAGACCTCACTCTACGGACAGTGCCACACATAGAAAGATGACTCTGTCACTTGCAGATAGGTGTTCAA AGACACAGAAGATTAGAATCTTGCCAATGGCTGGTCGTGATCCTGAATGCCAACGCACAGAAATGATTAA GAAAGAAGAAGAACGTTTGAGGGCTTCCATACGTAGGGAATCTCAGCAGCGCCGAATGAGAGAGAAACAG CACCAGCGGGGGCTGAGCGCCAGTTACCTGGAACCTGATCGATACGATGAGGAGGAGGAAGGCGAGGAGT CCATCAGCTTGGCTGCCATTAAAAACCGATATAAAGGGGGCATTCGAGAGGAACGAGCCAGAATCTATTC ATCAGACAGTGATGAGGGATCAGAAGAAGATAAAGCTCAAAGATTACTCAAAGCAAAGAAACTTACCAGT GATGAGGAAGGTGAACCTTCCGGAAAGAGAAAAGCAGAAGATGATGATAAAGCAAATAAAAAGCATAAGA AGTATGTGATCAGCGATGAAGAGGAAGAAGATGATGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG202274 representing NM_138792 Red=Cloning site Green=Tags(s) MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDERGDSGQPSNKELFGDDSEDEG ASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHS DDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEKA NSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDERPQASDEEHRHSDDEEEQDHKSESARGSDSE DEVLRMKRKNAIASDSEADSDTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEE EPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTI RWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLT FRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQ HQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLTS DEEGEPSGKRKAEDDDKANKKHKKYVISDEEEEDDD TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_138792
ORF Size	1998 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_138792.4
RefSeq Size	2162 bp
RefSeq ORF	2001 bp
Locus ID	123169
UniProt ID	Q8WVC0
Domains	Leo1
Gene Summary	LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC202274	LEO1 (Myc-DDK-tagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,990.00
RC202274L1	Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202274L2	Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202274L3	Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC202274L4	Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
SC120657	LEO1 (untagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 2,950.00

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