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LEO1 (NM_138792) Human Tagged ORF Clone
CAT＃: RC202274
LEO1 (Myc-DDK-tagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1)
ORF Plasmid: tGFP
"NM_138792" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RDL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202274 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATATGGAGGATCTCTTCGGGAGCGACGCCGACAGCGAAGCTGAGCGTAAAGATTCTGATTCTG GATCTGACTCAGATTCTGATCAAGAGAATGCTGCCTCTGGCAGTAATGCCTCTGGAAGTGAAAGTGATCA GGATGAAAGAGGTGATTCAGGACAACCAAGTAATAAGGAACTGTTTGGAGATGACAGTGAGGACGAGGGA GCTTCACATCATAGTGGTAGTGATAATCACTCTGAAAGATCAGACAATAGATCAGAAGCTTCTGAGCGTT CTGACCATGAGGACAATGACCCCTCAGATGTAGATCAGCACAGTGGATCAGAAGCCCCTAATGATGATGA AGACGAAGGTCATAGATCGGATGGAGGGAGCCATCATTCAGAAGCAGAAGGTTCTGAAAAAGCACATTCA GATGATGAAAAATGGGGCAGAGAAGATAAAAGTGACCAGTCAGATGATGAAAAGATACAAAATTCTGATG ATGAGGAGAGGGCACAAGGATCTGATGAAGATAAGCTGCAGAATTCTGACGATGATGAGAAAATGCAGAA CACAGATGATGAGGAGAGGCCTCAGCTTTCCGATGATGAGAGACAACAGCTATCTGAGGAGGAAAAGGCT AATTCTGATGATGAACGGCCGGTAGCTTCTGATAATGATGATGAGAAACAGAATTCTGATGATGAAGAAC AACCACAGCTGTCTGATGAAGAGAAAATGCAAAATTCTGATGATGAAAGGCCACAGGCCTCAGATGAAGA ACACAGGCATTCAGATGATGAAGAGGAACAGGATCATAAATCAGAATCTGCAAGAGGCAGTGATAGTGAA GATGAAGTTTTACGAATGAAACGCAAGAATGCGATTGCATCTGATTCAGAAGCGGATAGTGACACTGAGG TGCCAAAAGATAATAGTGGAACCATGGATTTATTTGGAGGTGCAGATGATATCTCTTCAGGGAGTGATGG AGAAGACAAACCACCTACTCCAGGACAGCCTGTTGATGAAAATGGATTGCCTCAGGATCAACAGGAAGAG GAGCCAATTCCTGAGACCAGAATAGAAGTAGAAATACCCAAAGTAAACACTGATTTAGGAAACGACTTAT ATTTTGTTAAACTGCCCAACTTTCTCAGTGTAGAGCCCAGACCTTTTGATCCTCAGTATTATGAAGATGA ATTTGAAGATGAAGAAATGCTGGATGAAGAAGGTAGAACCAGGTTAAAATTAAAGGTAGAAAATACTATA AGATGGAGGATACGCCGAGATGAAGAAGGAAATGAAATTAAAGAAAGCAATGCTCGGATAGTCAAGTGGT CAGATGGAAGCATGTCCCTGCATTTAGGCAATGAAGTGTTTGATGTGTACAAAGCCCCACTGCAGGGCGA CCACAATCATCTTTTTATAAGACAAGGTACTGGTCTACAGGGACAAGCAGTCTTTAAAACGAAACTCACC TTCAGACCTCACTCTACGGACAGTGCCACACATAGAAAGATGACTCTGTCACTTGCAGATAGGTGTTCAA AGACACAGAAGATTAGAATCTTGCCAATGGCTGGTCGTGATCCTGAATGCCAACGCACAGAAATGATTAA GAAAGAAGAAGAACGTTTGAGGGCTTCCATACGTAGGGAATCTCAGCAGCGCCGAATGAGAGAGAAACAG CACCAGCGGGGGCTGAGCGCCAGTTACCTGGAACCTGATCGATACGATGAGGAGGAGGAAGGCGAGGAGT CCATCAGCTTGGCTGCCATTAAAAACCGATATAAAGGGGGCATTCGAGAGGAACGAGCCAGAATCTATTC ATCAGACAGTGATGAGGGATCAGAAGAAGATAAAGCTCAAAGATTACTCAAAGCAAAGAAACTTACCAGT GATGAGGAAGGTGAACCTTCCGGAAAGAGAAAAGCAGAAGATGATGATAAAGCAAATAAAAAGCATAAGA AGTATGTGATCAGCGATGAAGAGGAAGAAGATGATGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202274 protein sequence
Red=Cloning site Green=Tags(s) MADMEDLFGSDADSEAERKDSDSGSDSDSDQENAASGSNASGSESDQDERGDSGQPSNKELFGDDSEDEG ASHHSGSDNHSERSDNRSEASERSDHEDNDPSDVDQHSGSEAPNDDEDEGHRSDGGSHHSEAEGSEKAHS DDEKWGREDKSDQSDDEKIQNSDDEERAQGSDEDKLQNSDDDEKMQNTDDEERPQLSDDERQQLSEEEKA NSDDERPVASDNDDEKQNSDDEEQPQLSDEEKMQNSDDERPQASDEEHRHSDDEEEQDHKSESARGSDSE DEVLRMKRKNAIASDSEADSDTEVPKDNSGTMDLFGGADDISSGSDGEDKPPTPGQPVDENGLPQDQQEE EPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTI RWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLT FRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQ HQRGLSASYLEPDRYDEEEEGEESISLAAIKNRYKGGIREERARIYSSDSDEGSEEDKAQRLLKAKKLTS DEEGEPSGKRKAEDDDKANKKHKKYVISDEEEEDDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138792 |
| ORF Size | 1998 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138792.4 |
| RefSeq Size | 2202 bp |
| RefSeq ORF | 2001 bp |
| Locus ID | 123169 |
| UniProt ID | Q8WVC0 |
| Domains | Leo1 |
| MW | 75.4 kDa |
| Gene Summary | LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PAF1 complex interactions with SETDB1 mediate promoter H3K9 methylation and transcriptional repression of Hoxa9 and Meis1 in acute myeloid leukemia
,Ropa, J;Saha, N;Chen, Z;Serio, J;Chen, W;Mellacheruvu, D;Zhao, L;Basrur, V;Nesvizhskii, AI;Muntean, AG;,
Oncotarget
,PubMed ID 29774127
[LEO1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202274L1 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202274L2 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged |
CNY 5,890.00 |
|
| RC202274L3 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202274L4 | Lenti ORF clone of Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1), mGFP tagged |
CNY 5,890.00 |
|
| RG202274 | LEO1 (tGFP-tagged) - Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) |
CNY 4,370.00 |
|
| SC120657 | LEO1 (untagged)-Human Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) (LEO1) |
CNY 2,950.00 |
