DTD1 (NM_080820) Human Untagged Clone

CAT#: SC120366

DTD1 (untagged)-Human D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (DTD1), nuclear gene encoding mitochondrial protein



  "NM_080820" in other vectors (4)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
DTD1 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms C20orf88; DTD; DUE-B; DUEB; HARS2; pqn-68
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC120366 sequence for NM_080820 edited (data generated by NextGen Sequencing)
ATGAAGGCCGTGGTGCAGCGCGTCACCCGGGCCAGCGTCACAGTTGGAGGAGAGCAGATT
AGTGCCATTGGAAGGGGCATATGTGTGTTGCTGGGTATTTCCCTGGAGGATACGCAGAAG
GAACTGGAACACATGGTCCGAAAGATTCTAAACCTGCGTGTATTTGAGGATGAGAGTGGG
AAGCACTGGTCGAAGAGTGTGATGGACAAACAGTACGAGATTCTGTGTGTCAGCCAGTTT
ACCCTCCAGTGTGTCCTGAAGGGAAACAAGCCTGATTTCCACCTAGCAATGCCCACGGAG
CAGGCAGAGGGCTTCTACAACAGCTTCCTGGAGCAGCTGCGTAAAACATACAGGCCGGAG
CTTATCAAAGATGGCAAGTTTGGGGCCTACATGCAGGTGCACATTCAGAATGATGGGCCT
GTGACCATAGAGCTGGAATCGCCAGCTCCCGGCACTGCTACCTCTGACCCAAAGCAGCTG
TCAAAGCTCGAAAAACAGCAGCAGAGGAAAGAAAAGACCAGAGCTAAGGGACCTTCTGAA
TCAAGCAAGGAAAGAAACACTCCCCGAAAAGAAGACCGCAGTGCCAGCAGCGGGGCTGAG
GGCGACGTGTCCTCTGAACGGGAGCCGTAG

Clone variation with respect to NM_080820.4
>OriGene 5' read for NM_080820 unedited
GTCAGATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGGCGCTTC
CGGGTGTCACCTCCAGAGGCGCCGGCTGCGGAGCCGCCCTCAGAGTCGCGAGGCCGGACG
CAGCGCGGCGCCGCCCCACTCGCCCCAGCCGCCGCCATGAAGGCCGTGGTGCAGCGCGTC
ACCCGGGCCAGCGTCACAGTTGGAGGAGAGCAGATTAGTGCCATTGGAAGGGGCATATGT
GTGTTGCTGGGTATTTCCCTGGAGGATACGCAGAAGGAACTGGAACACATGGNTCCGAAA
GATTCTAAACCTGCGTGTATTTGAGGATGAGAGTGGGAAGCACTGGTCGAAGAGTGTGAT
GGACAAACAGTACGAGATTCTGTGTGTCAGCCAGTTTACCCTCCAGTGTGTCCTGAAGGG
AAACAAGCCTGATTTCCACCTAGCAATGCCCACGGAGCAGGCAGAGGGCTTCTACAACAG
CTTCCTGGAGCAGCTGCGTAAAACATACAGGCCGGAGCTTATCAAAGATGGCAAGTTTGG
GGCCTACATGCAGGTGCACATTCAGAATGATGGGCCTGTGACCATAGAGCTGGAATCGCC
AGCTCCCGGCACTGCTACCTCTGACCCAAAGCAGCTGTCAAAGCTCGAAAAACAGCAGCA
GAGGAAAGAAAAGACCAGAGCTAAGGGACCTTCTGAATCAAGCAAGGAAAGAAACACTTC
CCCGAAAGAAGACCGCAGTGCCAGCAGCGGNGCTGAGGGCGACGTGTCCTCTGAACGGGA
GCCGTAGCTCANGAGGCAGAATTTCAGTGTGTATCATTGNGCAGAACTGGATCCCTGAAA
ANNTCAGATGCTAGCACCTACACTACTTNTAGAAATTTGGACTGTAACATGAAGAGGGAG
ACAGNNAATAGAATTTGGGGACCTCGATAGCT
>OriGene 3' read for NM_080820 unedited
CCCCTCGGTCCCCCCGCCCCCCCCCCCCCACCCTTACTCTGNACCCGGCCGCATTCTANG
ATCGGTTTTTTTCTTTTTTTTTTGGGGACCTATTATTATTTATTTTTAGAAAAAAGAGAA
AATTTACAAGATGCAAAGGTCTCACACAATACATTATCATTGATGATTGCTCTCTTGACA
CTTCAACACGGGCCCTGGCAATAAAAAGAACATGGACAGTGTGAGTGTATTGGAGAACCC
CCGCCATGGACACGCAGAATACACCACAGTGGACTTGATGACAAATGATGCTGGGCCAGC
CTGTTCCAAAAGCAAGCCAAGATGTGGTAGAAACCAACAAAGCCTTTCTGTGGGGGAGGC
ACGAGCGCACACCTTTTACCACATGCGCACCCGCCCCCTGGCCCACCGGCAGTGCCCACT
GGATCACTCCACCACAGCAACAACAGAAAACGATAAAACGGTCCTTTGGTGTTTTTTGCA
CAGCTATTCAGGTTCCCAAATTCTTATTTCTGGCTTCCTCTTCATGGTTCAGNTCCCAAA
CTTTTAAAGCAGTGGACGCGCTTAGCCATTCTTGAATTTTTCAGGATCCAGCCCCTGCCC
AAATGATAACACACTGAACTCTGCCTTCCTGAGCTACGGCTTCCGGTCAGAGGACACCGT
CGACTTCAACCCCGCTGCTGGCACTGCCGGCTTCTTTTCGGGGGAGATGGTTCCTTCCCT
TGCTTGAACCAGAAAGTCCCCTTAGCTCCTGGGCTTTTCCTTCCCCCGCCGGCTGCTTTT
CGAACCTTTGACAGTTGCTTTTGGGCCAAAAGGTAACCACTGCCCGGAAGCCTGGGGATT
TCAGCTTTCATGGCAAAAAGGCCCATCAATTTCGAAAGGGCCCCTGGATGGACGCCCCCA
AATTGGCCTATTTTGCATAACCCCCGCCCTGGTTGCTTTACCCCATGTGTCACG
Restriction Sites NotI-NotI     
ACCN NM_080820
Insert Size 1300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_080820.3, NP_543010.3
RefSeq Size 1344 bp
RefSeq ORF 630 bp
Locus ID 92675
UniProt ID Q8TEA8
Domains DUF154
Gene Summary The protein encoded by this gene is similar in sequence to histidyl-tRNA synthetase, which hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). The encoded protein binds the DNA unwinding element and plays a role in the initiation of DNA replication. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (2) has a different 3' structure, resulting in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus, and is shorter, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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