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DTD1 (NM_080820) Human Tagged ORF Clone
CAT＃: RC207233
DTD1 (Myc-DDK-tagged)-Human D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (DTD1), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_080820" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C20orf88; DTD; DUE-B; DUEB; HARS2; pqn-68 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207233 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCCGTGGTGCAGCGCGTCACCCGGGCCAGCGTCACAGTTGGAGGAGAGCAGATTAGTGCCATTG GAAGGGGCATATGTGTGTTGCTGGGTATTTCCCTGGAGGATACGCAGAAGGAACTGGAACACATGGTCCG AAAGATTCTAAACCTGCGTGTATTTGAGGATGAGAGTGGGAAGCACTGGTCGAAGAGTGTGATGGACAAA CAGTACGAGATTCTGTGTGTCAGCCAGTTTACCCTCCAGTGTGTCCTGAAGGGAAACAAGCCTGATTTCA ACCTAGCAATGCCCACGGAGCAGGCAGAGGGCTTCTACAACAGCTTCCTGGAGCAGCTGCGTAAAACATA CAGGCCGGAGCTTATCAAAGATGGCAAGTTTGGGGCCTACATGCAGGTGCACATTCAGAATGATGGGCCT GTGACCATAGAGCTGGAATCGCCAGCTCCCGGCACTGCTACCTCTGACCCAAAGCAGCTGTCAAAGCTCG AAAAACAGCAGCAGAGGAAAGAAAAGACCAGAGCTAAGGGACCTTCTGAATCAAGCAAGGAAAGAAACAC TCCCCGAAAAGAAGACCGCAGTGCCAGCAGCGGGGCTGAGGGCGACGTGTCCTCTGAACGGGAGCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207233 protein sequence
Red=Cloning site Green=Tags(s) MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDK QYEILCVSQFTLQCVLKGNKPDFNLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGP VTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080820 |
| ORF Size | 627 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080820.6 |
| RefSeq Size | 1368 bp |
| RefSeq ORF | 630 bp |
| Locus ID | 92675 |
| UniProt ID | Q8TEA8 |
| Domains | DUF154 |
| MW | 23.4 kDa |
| Gene Summary | The protein encoded by this gene is similar in sequence to histidyl-tRNA synthetase, which hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). The encoded protein binds the DNA unwinding element and plays a role in the initiation of DNA replication. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207233L3 | Lenti ORF clone of Human D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (DTD1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207233L4 | Lenti ORF clone of Human D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (DTD1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG207233 | DTD1 (tGFP-tagged) - Human D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (DTD1), nuclear gene encoding mitochondrial protein |
CNY 4,000.00 |
|
| SC120366 | DTD1 (untagged)-Human D-tyrosyl-tRNA deacylase 1 homolog (S. cerevisiae) (DTD1), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
