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Niemann Pick C1 (NPC1) (NM_000271) Human Untagged Clone
CAT＃: SC120010
NPC1 (untagged)-Human Niemann-Pick disease, type C1 (NPC1)
CNY 13,360.00
货期*
现货
规格
| Cited in 5 publications. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NPC; POGZ; SLC65A1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000271 edited
CCGGCGTCCGCAGCCTTGCGCGGCCACAGCATGACCGCTCGCGGCCTGGCCCTTGGCCTC CTCCTGCTGCTACTGTGTCCAGCGCAGGTGTTTTCACAGTCCTGTGTTTGGTATGGAGAG TGTGGAATTGCATATGGGGACAAGAGGTACAATTGCGAATATTCTGGCCCACCAAAACCA TTGCCAAAGGATGGATATGACTTAGTGCAGGAACTCTGTCCAGGATTCTTCTTTGGCAAT GTCAGTCTCTGTTGTGATGTTCGGCAGCTTCAGACACTAAAAGACAACCTGCAGCTGCCT CTACAGTTTCTGTCCAGATGTCCATCCTGTTTTTATAACCTACTGAACCTGTTTTGTGAG CTGACATGTAGCCCTCGACAGAGTCAGTTTTTGAATGTTACAGCTACTGAAGATTATGTT GATCCTGTTACAAACCAGACGAAAACAAATGTGAAAGAGTTACAATACTACGTCGGACAG AGTTTTGCCAATGCAATGTACAATGCCTGCCGGGATGTGGAGGCCCCCTCAAGTAATGAC AAGGCCCTGGGACTCCTGTGTGGGAAGGACGCTGACGCCTGTAATGCCACCAACTGGATT GAATACATGTTCAATAAGGACAATGGACAGGCACCTTTTACCATCACTCCTGTGTTTTCA GATTTTCCAGTCCATGGGATGGAGCCCATGAACAATGCCACCAAAGGCTGTGACGAGTCT GTGGATGAGGTCACAGCACCATGTAGCTGCCAAGACTGCTCTATTGTCTGTGGCCCCAAG CCCCAGCCCCCACCTCCTCCTGCTCCCTGGACGATCCTTGGCTTGGACGCCATGTATGTC ATCATGTGGATCACCTACATGGCGTTTTTGCTTGTGTTTTTTGGAGCATTTTTTGCAGTG TGGTGCTACAGAAAACGGTATTTTGTCTCCGAGTACACTCCCATCGATAGCAATATAGCT TTTTCTGTTAATGCAAGTGACAAAGGAGAGGCGTCCTGCTGTGACCCTGTCAGCGCAGCA TTTGAGGGCTGCTTGAGGCGGCTGTTCACACGCTGGGGGTCTTTCTGCGTCCGAAACCCT GGCTGTGTCATTTTCTTCTCGCTGGTCTTCATTACTGCGTGTTCGTCAGGCCTGGTGTTT GTCCGGGTCACAACCAATCCAGTTGACCTCTGGTCAGCCCCCAGCAGCCAGGCTCGCCTG GAAAAAGAGTACTTTGACCAGCACTTTGGGCCTTTCTTCCGGACGGAGCAGCTCATCATC CGGGCCCCTCTCACTGACAAACACATTTACCAGCCATACCCTTCGGGAGCTGATGTACCC TTTGGACCTCCGCTTGACATACAGATACTGCACCAGGTTCTTGACTTACAAATAGCCATC GAAAACATTACTGCCTCTTATGACAATGAGACTGTGACACTTCAAGACATCTGCTTGGCC CCTCTTTCACCGTATAACACGAACTGCACCATTTTGAGTGTGTTAAATTACTTCCAGAAC AGCCATTCCGTGCTGGACCACAAGAAAGGGGACGACTTCTTTGTGTATGCCGATTACCAC ACGCACTTTCTGTACTGCGTACGGGCTCCTGCCTCTCTGAATGATACAAGTTTGCTCCAT GACCCTTGTCTGGGTACGTTTGGTGGACCAGTGTTCCCGTGGCTTGTGTTGGGAGGCTAT GATGATCAAAACTACAATAACGCCACTGCCCTTGTGATTACCTTCCCTGTCAATAATTAC TATAATGATACAGAGAAGCTCCAGAGGGCCCAGGCCTGGGAAAAAGAGTTTATTAATTTT GTGAAAAACTACAAGAATCCCAATCTGACCATTTCCTTCACTGCTGAACGAAGTATTGAA GATGAACTAAATCGTGAAAGTGACAGTGATGTCTTCACCGTTGTAATTAGCTATGCCATC ATGTTTCTATATATTTCCCTAGCCTTGGGGCACATCAAAAGCTGTCGCAGGCTTCTGGTG GATTCGAAGGTCTCACTAGGCATCGCGGGCATCTTGATCGTGCTGAGCTCGGTGGCTTGC TCCTTGGGTGTCTTCAGCTACATTGGGTTGCCCTTGACCCTCATTGTGATTGAAGTCATC CCGTTCCTGGTGCTGGCTGTTGGAGTGGACAACATCTTCATTCTGGTGCAGGCCTACCAG AGAGATGAACGTCTTCAAGGGGAAACCCTGGATCAGCAGCTGGGCAGGGTCCTAGGAGAA GTGGCTCCCAGTATGTTCCTGTCATCCTTTTCTGAGACTGTAGCATTTTTCTTAGGAGCA TTGTCCGTGATGCCAGCCGTGCACACCTTCTCTCTCTTTGCGGGATTGGCAGTCTTCATT GACTTTCTTCTGCAGATTACCTGTTTCGTGAGTCTCTTGGGGTTAGACATTAAACGTCAA GAGAAAAATCGGCTAGACATCTTTTGCTGTGTCAGAGGTGCTGAAGATGGAACAAGCGTC CAGGCCTCAGAGAGCTGTTTGTTTCGCTTCTTCAAAAACTCCTATTCTCCACTTCTGCTA AAGGACTGGATGAGACCAATTGTGATAGCAATATTTGTGGGTGTTCTGTCATTCAGCATC GCAGTCCTGAACAAAGTAGATATTGGATTGGATCAGTCTCTTTCGATGCCAGATGACTCC TACATGGTGGATTATTTCAAATCCATCAGTCAGTACCTGCATGCGGGTCCGCCTGTGTAC TTTGTCCTGGAGGAAGGGCACGACTACACTTCTTCCAAGGGGCAGAACATGGTGTGCGGC GGCATGGGCTGCAACAATGATTCCCTGGTGCAGCAGATATTTAACGCGGCGCAGCTGGAC AACTATACCCGAATAGGCTTCGCCCCCTCGTCCTGGATCGACGATTATTTCGACTGGGTG AAGCCACAGTCGTCTTGCTGTCGAGTGGACAATATCACTGACCAGTTCTGCAATGCTTCA GTGGTTGACCCTGCCTGCGTTCGCTGCAGGCCTCTGACTCCGGAAGGCAAACAGAGGCCT CAGGGGGGAGACTTCATGAGATTCCTGCCCATGTTCCTTTCGGATAACCCTAACCCCAAG TGTGGCAAAGGGGGACATGCTGCCTATAGTTCTGCAGTTAACATCCTCCTTGGCCATGGC ACCAGGGTCGGAGCCACGTACTTCATGACCTACCACACCGTGCTGCAGACCTCTGCTGAC TTTATTGACGCTCTGAAGAAAGCCCGACTTATAGCCAGTAATGTCACCGAAACCATGGGC ATTAACGGCAGTGCCTACCGAGTATTTCCTTACAGTGTGTTTTATGTCTTCTACGAACAG TACCTGACCATCATTGACGACACTATCTTCAACCTCGGTGTGTCCCTGGGCGCGATATTT CTGGTGACCATGGTCCTCCTGGGCTGTGAGCTCTGGTCTGCAGTCATCATGTGTGCCACC ATCGCCATGGTCTTGGTCAACATGTTTGGAGTTATGTGGCTCTGGGGCATCAGTCTGAAC GCTGTATCCTTGGTCAACCTGGTGATGAGCTGTGGCATCTCCGTGGAGTTCTGCAGCCAC ATAACCAGAGCGTTCACGGTGAGCATGAAAGGCAGCCGCGTGGAGCGCGCGGAAGAGGCA CTTGCCCACATGGGCAGCTCCGTGTTCAGTGGAATCACACTTACAAAATTTGGAGGGATT GTGGTGTTGGCTTTTGCCAAATCTCAAATTTTCCAGATATTCTACTTCAGGATGTATTTG GCCATGGTCTTACTGGGAGCCACTCACGGATTAATATTTCTCCCTGTCTTACTCAGTTAC ATAGGGCCATCAGTAAATAAAGCCAAAAGTTGTGCCACTGAAGAGCGATACAAAGGAACA GAGCGCGAACGGCTTCTAAATTTCTAGCCCTCTCGCAGGGCATCCTGACTGAACTGTGTC TAAGGGTCGGTCGGTTTACCACTGGACGGGTGCTGCATCGGCAAGGCCAAGTTGAACACC GGATGGTGCCAACCATCGGTTGTTTGGCAGCAGCTTTGAACGTAGCGCCTGTGAACTCAG GAATGCACAGTTGACTTGGGAAGCAGTATTACTAGATCTGGAGGCAACCACAGGACACTA AACTTCTCCCAGCCTCTTCAGGAAAGAAACCTCATTCTTTGGCAAGCAGGAGGTGACACT AGATGGCTGTGAATGTGATCCGCTCACTGACACTCTGTAAAGGCCAATCAATGCACTGTC TGTCTCTCCTTTTAGGAGTAAGCCATCCCACAAGTTCTATACCATATTTTTAGTGACAGT TGAGGTTGTAGATACACTTTATAACATTTTATAGTTTAAAGAGCTTTATTAATGCAATAA ATTAACTTTGTACACATTTTTATATAAAAAAACAGCAAGTGATTTCAGAATGTTGTAGGC CTCATTAGAGCTTGGTCTCCAAAAATCTGTTTGAAAAAAGCAACATGTTCTTCACAGTGT TCCCCTAGAAAGGAAGAGATTTAATTGCCAGTTAGATGTGGCATGAAATGAGGGACAAAG AAAGCATCTCGTAGGTGTGTCTACTGGGTTTTAACTTATTTTTCTTTAATAAAATACATT GTTTTCCTAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_000271 unedited
GCCGCGAATTCGGCACGAGCCGGCGTCCGCAGCCTTGCGCGGCCACAGCATGACCGCTCG CGGCCTGGCCCTTGGCCTCCTCCTGCTGCTACTGTGTCCAGCGCAGGTGTTTTCACAGTC CTGTGTTTGGTATGGAGAGTGTGGAATTGCATATGGGGACAAGAGGTACAATTGCGAATA TTCTGGCCCACCAAAACCATTGCCAAAGGATGGATATGACTTAGTGCAGGAACTCTGTCC AGGATTCTTCTTTGGCAATGTCAGTCTCTGTTGTGATGTTCGGCAGCTTCAGACACTAAA AGACAACCTGCAGCTGCCTCTACAGTTTCTGTCCAGATGTCCATCCTGTTTTTATAACCT ACTGAACCTGTTTTGTGAGCTGACATGTAGCCCTCGACAGAGTCAGTTTTTGAATGTTAC AGCTACTGAAGATTATGTTGATCCTGTTACAAACCAGACGAAAACAAATGTGAAAGAGTT ACAATACTACGTCGGACAGAGTTTTGCCAATGCAATGTACAATGCCTGCCGGGATGTGGA GGCCCCCTCAAGTAATGACAAGGCCCTGGGACTCCTGTGTGGGAAGGACGCTGACGCCTG TAATGCCACCAACTGGATTGAATACATGTTCAATAAGGACAATGGACAGGCACCTTTTAC CATCACTCCTGTGNTTTCAGATTTTCCAGTCCATGGGATGGAGCCCATGAACAATGCCAC CN >OriGene 3' read for NM_000271 unedited
TGGACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGGAAAACAATGTA TTTTATTAAAGAAAAATAAGTTAAAACCCAGTAGACACACCTACGAGATGCTTTCTTTGT CCCTCATTTCATGCCACATCTAACTGGCAATTAAATCTCTTCCTTTCTAGGGGAACACTG TGAAGAACATGTTGCTTTTTTCAAACAGATTTTTGGAGACCAAGCTCTAATGAGGCCTAC AACATTCTGAAATCACTTGCTGTTTTTTTATATAAAAATGTGTACAAAGTTAATTTATTG CATTAATAAAGCTCTTTAAACTATAAAATGTTATAAAGTGTATCTACAACCTCAACTGTC ACTAAAAATATGGTATAGAACTTGTGGGATGGCTTACTCCTAAAAGGAGAGACAGACAGT GCATTGATTGGCCTTTACAGAGTGTCAGTGAGCGGATAACCATTACCAGCCTCTAGTGTC CCCTCCTGCTTCCCAAAGAACGAGGCTTTTTTTCTCACCAGGCTGCGACAACTTTAGTGT CCTGCGGTCGCCCCACATTTACAATACCGCCCCCCAGGCCCCCCGTTTTCCCTGACTCTC AGGCCCTCCCCCCCACCTGCTGCCCCACACCCGATCGGCACCACCACTCGCCCGTCCTCC ATGAGCATTTACAACCCTCCCCCCTTCTCCCCCCTTCCCCCCCCCTTCTCCCCCTTCCCC CCGCCTCCCTCTCCCTTCCCCCGTCCCCCATTCCCTCCCCCTTTCCTCCCCCTACATCCA TGCCCCCCTTTTCTTTTTTTCCTCCGTCATATTCTCCCCCTCTCTCCCCACCTTTCCTCC CCCCTTTCCCTCCTCCCTCCCTCCACCCCCCCGCACAATCGTGCACACCCCTCCATATTC TATCCCCCCCTCCCATCATTCTTCCCCCCTCCCCTCCTCCGTTACCCTCCCTCCATCGCC CCCCCGTCCTTGTTACCGCCCACACCACCTTTATCGCACCCCCCTGCCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000271 |
| Insert Size | 4800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000271.1, NP_000262.1 |
| RefSeq Size | 4673 bp |
| RefSeq ORF | 3837 bp |
| Locus ID | 4864 |
| UniProt ID | O15118 |
| Domains | Patched |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Lysosome |
| Gene Summary | This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.[provided by RefSeq, Aug 2009] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Plasmid DNA gene therapy of the Niemann-Pick C1 mouse with transferrin receptor-targeted Trojan horse liposomes
,Jiang, D;Lee, H;Pardridge, WM;,
Sci Rep
,PubMed ID 32770132
[NPC1]
|
|
An exploration of conditions proposed to trigger the Ebola virus glycoprotein for fusion
,Fénéant, L;Szymańska-de Wijs, KM;Nelson, EA;White, JM;,
PLoS ONE
,PubMed ID 31276481
[NPC1]
|
|
Deficiency of Niemann-Pick Type C-1 Protein Impairs Release of Human Immunodeficiency Virus Type 1 and Results in Gag Accumulation in Late Endosomal/Lysosomal Compartments
,Yuyang Tang, Ihid Carneiro Leao, Ebony M. Coleman, Robin Shepard Broughton, and James E. K. Hildreth,
J. Virol., Aug 2009; 83: 7982 - 7995.
[NPC1]
|
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NPC1 repression contributes to lipid accumulation in human macrophages exposed to environmental aryl hydrocarbons
,Normand Podechard, Eric Le Ferrec, Amélie Rebillard, Olivier Fardel, and Valérie Lecureur,
Cardiovasc Res, Jan 2009; 10.1093/cvr/cvp007.
[NPC1]
|
|
Purified NPC1 Protein: I. BINDING OF CHOLESTEROL AND OXYSTEROLS TO A 1278-AMINO ACID MEMBRANE PROTEIN
,Rodney E. Infante, Lina Abi-Mosleh, Arun Radhakrishnan, Jarrod D. Dale, Michael S. Brown, and Joseph L. Goldstein,
J. Biol. Chem. 2008; 283(2): p. 1052-1063
[NPC1]
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