Niemann Pick C1 (NPC1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Electron Microscopy, ICC/IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry: 5-10 ug/ml, Immunohistochemistry-Paraffin: 5-10 ug/ml, Knockdown Validated, Knockout Validated, Electron Microscopy, Immunocytochemistry/ Immunofluorescence: 1:250, Western Blot: 1:1000-1:3000, Immunoprecipitation: 1:10-1:500
Reactivity	Human, Mouse, Rat, Chinese Hamster, Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to the C-terminal region of human Niemann-Pick C. [UniProt# O15118]
Formulation	PBS, 0.1% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	NPC intracellular cholesterol transporter 1
Database Link	NP_000262 Entrez Gene 18145 MouseEntrez Gene 266732 RatEntrez Gene 4864 Human UniProt ID O15118
Background	Niemann-Pick type C1 (NPC1) is a member of a family of genes encoding membrane-bound proteins containing putative sterol sensing domains. NPC1 protein regulates cholesterol transport from late endosomes-lysosomes to other intracellular compartments. NPC1 overexpression increases the rate of trafficking of low density lipoprotein cholesterol to the endoplasmic reticulum and the rate of delivery of endosomal cholesterol to the plasma membrane. NPC disease is an inherited neurovisceral lipid storage disorder of unesterified cholesterol accumulation in lysosomes. It is characterized by progressive neural and liver degeneration, resulting from inactivating mutations in NPC1, in most cases.
Synonyms	NPC
Note	This Niemann-Pick C1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, Immunoprecipitation and Western blot. In Western blot the antibody detects heterogeneously glycosylated NPC1 protein with prominent bands at 170 and 220 kDa. It has also been tested for immuno-EM (on human protein only). Use in Electron Microscopy reported in scientific literature (PMID: 21051527)
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Lysosome

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.