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Glucose 6 Phosphate Dehydrogenase (G6PD) (NM_000402) Human Untagged Clone
CAT＃: SC119906
G6PD (untagged)-Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1
CNY 5,872.00
CNY 8,740.00
| Cited in 3 publications. |
Product images
CNY 5,381.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | G6PD1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_000402 edited
ATGGGCCGGCGGGGCTCAGCCCCCGGAAACGGTCGTACACTTCGGGGCTGCGAGCGCGGA GGGCGACGACGACGAAGCGCAGACAGCGTCATGGCAGAGCAGGTGGCCCTGAGCCGGACC CAGGTGTGCGGGATCCTGCGGGAAGAGCTTTTCCAGGGCGATGCCTTCCATCAGTCGGAT ACACACATATTCATCATCATGGGTGCATCGGGTGACCTGGCCAAGAAGAAGATCTACCCC ACCATCTGGTGGCTGTTCCGGGATGGCCTTCTGCCCGAAAACACCTTCATCGTGGGCTAT GCCCGTTCCCGCCTCACAGTGGCTGACATCCGCAAACAGAGTGAGCCCTTCTTCAAGGCC ACCCCAGAGGAGAAGCTCAAGCTGGAGGACTTCTTTGCCCGCAACTCCTATGTGGCTGGC CAGTACGATGATGCAGCCTCCTACCAGCGCCTCAACAGCCACATGAATGCCCTCCACCTG GGGTCACAGGCCAACCGCCTCTTCTACCTGGCCTTGCCCCCGACCGTCTACGAGGCCGTC ACCAAGAACATTCACGAGTCCTGCATGAGCCAGATAGGCTGGAACCGCATCATCGTGGAG AAGCCCTTCGGGAGGGACCTGCAGAGCTCTGACCGGCTGTCCAACCACATCTCCTCCCTG TTCCGTGAGGACCAGATCTACCGCATCGACCACTACCTGGGCAAGGAGATGGTGCAGAAC CTCATGGTGCTGAGATTTGCCAACAGGATCTTCGGCCCCATCTGGAACCGGGACAACATC GCCTGCGTTATCCTCACCTTCAAGGAGCCCTTTGGCACTGAGGGTCGCGGGGGCTATTTC GATGAATTTGGGATCATCCGGGACGTGATGCAGAACCACCTACTGCAGATGCTGTGTCTG GTGGCCATGGAGAAGCCCGCCTCCACCAACTCAGATGACGTCCGTGATGAGAAGGTCAAG GTGTTGAAATGCATCTCAGAGGTGCAGGCCAACAATGTGGTCCTGGGCCAGTACGTGGGG AACCCCGATGGAGAGGGCGAGGCCACCAAAGGGTACCTGGACGACCCCACGGTGCCCCGC GGGTCCACCACCGCCACTTTTGCAGCCGTCGTCCTCTATGTGGAGAATGAGAGGTGGGAT GGGGTGCCCTTCATCCTGCGCTGCGGCAAGGCCCTGAACGAGCGCAAGGCCGAGGTGAGG CTGCAGTTCCATGATGTGGCCGGCGACATCTTCCACCAGCAGTGCAAGCGCAACGAGCTG GTGATCCGCGTGCAGCCCAACGAGGCCGTGTACACCAAGATGATGACCAAGAAGCCGGGC ATGTTCTTCAACCCCGAGGAGTCGGAGCTGGACCTGACCTACGGCAACAGATACAAGAAC GTGAAGCTCCCTGACGCCTACGAGCGCCTCATCCTGGACGTCTTCTGCGGGAGCCAGATG CACTTCGTGCGCAGCGACGAGCTCCGTGAGGCCTGGCGTATTTTCACCCCACTGCTGCAC CAGATTGAGCTGGAGAAGCCCAAGCCCATCCCCTATATTTATGGCAGCCGAGGCCCCACG GAGGCAGACGAGCTGATGAAGAGAGTGGGTTTCCAGTATGAGGGCACCTACAAGTGGGTG AACCCCCACAAGCTCTGA >OriGene 5' read for NM_000402 unedited
AGCATATTTGTATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCCGCCCCCG CCGATTAAATGGGCCGGCGGGGCTCAGCCCCCGGAAACGGTCGTACACTTCGGGGCTGCG AGCGCGGAGGGCGACGACGACGAAGCGCAGACAGCGTCATGGCAGAGCAGGTGGCCCTGA GCCGGACCCAGGTGTGCGGGATCCTGCGGGAAGAGCTTTTCCAGGGCGATGCCTTCCATC AGTCGGATACACACATATTCATCATCATGGGTGCATCGGGTGACCTGGCCAAGAAGAAGA TCTACCCCACCATCTGGTGGCTGTTCCGGGATGGCCTTCTGCCCGAAAACACCTTCATCG TGGGCTATGCCCGTTCCCGCCTCACAGTGGCTGACATCCGCAAACAGAGTGAGCCCTTCT TCAAGGCCACCCCAGAGGAGAAGCTCAAGCTGGAGGACTTCTTTGCCCGCAACTCCTATG TGGCTGGCCAGTACGATGATGCAGCCTCCTACCAGCGCCTCAACAGCCACATGAATGCCC TCCACCTGGGGTCACAGGCCAACCGCCTCTTCTACCTGGCCTTGCCCCCGACCGTCTACG AGGCCGTCACCAAGAACATTCACGAGTCCTGCATGAGCCAGATAGGCTGGAACCGCATCA TCGTGGAGAAGCCCTTCGGGAGGGACCTGCAGAGCTCTGACCGGCTGTCCAACCACATCT CCTCCCTGTTCCGTGAGGACCAGATCTACCGCATCGACCACTACCTGGGCAAGGAGATGG TGCAGACCCTCATGGTGCTGAGATTTGCCCACAGGATCTTTCGCCCCATCTGGACCGGGA CACATCGNCTGCGTTATCCTCACCTTCAGGNAGCCCTTTTGCACTGAGGGTCGCGGNGGC TATTTCGATGAATTTGGGAAATTCCGGACGTGATGCAGAACACCTACTGCAGAGCTGNGT CTGG >OriGene 3' read for NM_000402 unedited
CCCCCCCCAATTAGTGTGCNCNCGCGGCCCGCTATTCTAGGACTCGGTTTTTTTTTTTTT TTTTTGGCTGTTTGCGGATTTAATGGCAGGGCATTGAGGTTGGGAGGGGTCCCAGCTGCT GCGTCTGCTTTTCTTATAGCAGAGAGGCTGCCTACGGGTGGCACGGGGTGGCCATGGAGT GCAGAGTTGGTGGGACAGGGGACATCCAGGGGGCTCGAGATGTTGCTGGTGACAAGGAAT GTCAAGTGGCACTGAGGCTGGCGGGCAAGGCCACAGGCAGATTCTCTCACGTGGGTGCTC GCCCCTTTCCTCCCCCTTGTCCCTCCCTCCCACCCTGGCCCCACTCAGGAGTGAGACCCA GTGGCCAATAAGCTCTGGGACAGACGAATGGGCGCCCTCCTCCTTCCTTCTGTTGGGCTG GAGTGAGTGGAGGAGGTGACTCAGCTCCTGGGCTCAGGCAGGGTCTGGAGGGGCCAGGAT GGTCTCGAGTGCTTGGCAGCTGAGGAATGTAGCTGGGCTCGGGTAGTAGCAGCAGCGAGG GGCGGGCCAGGGTGGCCAGAGCCCGGGGCCAGGAATGTGCAGCTGAGGTCAATGGTCCCG GAGTCCTCCCGACTCGGGGTCGGGCGGCGGGAAGGAGGGTGGCCGTGGCGGGGGTGGAGG TGGGTGCCCAGGGCTCACAGCTTGTGGGGGGTCACCCACTTGTAGGTGCCCTCATACTGG AAACCACTCTCTTCATCAGCTCGTCTGCCTCCGTGGGGCCTCCGCTGCCATAATATAGGG GATGGGCCTTGGGCTTTTCCAGCTCAATCTGGGCCCCCCCGGGGTGAAAATACGCCAGGC CTTACGGAACTTGTCGCTGTGCCCCAAGGCATTTGGGTTCCTGAGAACACTTCCAGAATG AGCGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000402 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000402.2, NP_000393.2 |
| RefSeq Size | 2650 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 2539 |
| UniProt ID | P11413 |
| Domains | G6PD |
| Protein Families | Druggable Genome |
| Protein Pathways | Glutathione metabolism, Metabolic pathways, Pentose phosphate pathway |
| Gene Summary | This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This full-length 545 aa form has been reported to be inactive, but may be processed to the smaller (515 aa) active form (PMID:8466644). |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
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Glucose-6-phosphate dehydrogenase increases Ca2+ currents by interacting with Cav1.2 and reducing intrinsic inactivation of the L-type calcium channel
,Gupte, R;Dhagia, V;Rocic, P;Ochi, R;Gupte, SA;,
Am. J. Physiol. Heart Circ. Physiol.
,PubMed ID 32442021
[G6PD]
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High glucose-induced ubiquitylation of G6PD leads to the injury of podocyte
,Zhang, Z;Wang, M;Hu, J;Yan, L;Yang, Y;He, M;Guo, S;Wu, M;Li, Q;Gong, W;Yang, Y;Handy, D;Lu, B;Hao, C;Wang, Q;Li, Y;Hu, R;Stanton, R;,
bioRxiv
[G6PD]
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High glucose inhibits glucose-6-phosphate dehydrogenase, leading to increased oxidative stress and {beta}-cell apoptosis
,Zhaoyun Zhang, Chong Wee Liew, Diane E. Handy, Yingyi Zhang, Jane A. Leopold, Ji Hu, Lili Guo, Rohit N. Kulkarni, Joseph Loscalzo, and Robert C. Stanton,
FASEB J, Dec 2009; 10.1096/fj.09-136572
[G6PD]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220625 | G6PD (Myc-DDK-tagged)-Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1 |
CNY 4,056.00 |
|
| RC220625L1 | Lenti ORF clone of Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1, Myc-DDK-tagged |
CNY 6,456.00 |
|
| RC220625L2 | Lenti ORF clone of Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1, mGFP tagged |
CNY 6,650.00 |
|
| RC220625L3 | Lenti ORF clone of Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1, Myc-DDK-tagged |
CNY 6,456.00 |
|
| RC220625L4 | Lenti ORF clone of Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1, mGFP tagged |
CNY 6,650.00 |
|
| RG220625 | G6PD (tGFP-tagged) - Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1 |
CNY 5,656.00 |
|
| SC317927 | G6PD (untagged)-Human glucose-6-phosphate dehydrogenase (G6PD), transcript variant 1 |
CNY 8,740.00 |
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