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FHIT (NM_002012) Human Untagged Clone
CAT＃: SC118885
FHIT (untagged)-Human fragile histidine triad gene (FHIT), transcript variant 1
CNY 1,200.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AP3Aase; FRA3B |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118885 sequence for NM_002012 edited (data generated by NextGen Sequencing)
ATGTCTTTCAGATTTGGCCAACATCTCATCAAGCCCTCTGTAGTGTTTCTCAAAACAGAA CTGTCCTTCGCTCTTGTGAATAGGAAACCTGTGGTACCAGGACATGTCCTTGTGTGCCCG CTGCGGCCAGTGGAGCGCTTCCATGACCTGCGTCCTGATGAAGTGGCCGATTTGTTTCAG ACGACCCAGAGAGTCGGGACAGTGGTGGAAAAACATTTCCATGGGACCTCTCTCACCTTT TCCATGCAGGATGGCCCCGAAGCCGGACAGACTGTGAAGCACGTTCACGTCCACGTTCTT CCCAGGAAGGCTGGAGACTTTCACAGGAATGACAGCATCTATGAGGAGCTCCAGAAACAT GACAAGGAGGACTTTCCTGCCTCTTGGAGATCAGAGGAGGAAATGGCAGCAGAAGCCGCA GCTCTGCGGGTCTACTTTCAGTGA Clone variation with respect to NM_002012.2 6 g=>t;294 t=>c >OriGene 5' read for NM_002012 unedited
NGGGTTCAAAATTTGNTATACGACTCACTATAGGGCGGCCGCGNAATCGGCACGAGGCTC TGTCCGGTCACAGGACTTTTTGCCCTCTGTTCCCGGGTCCCTCAGGCGGCCACCCAGTGG GCACACTCCCAGGCGGCGCTCCGGCCCCGCGCTCCCTCCCTCTGCCTTTCATTCCCAGCT GTCAACATCCTGGAAGCTTTGAAGCTCAGGAAAGAAGAGAAATCCACTGAGAACAGTCTG TAAAGGTCCGTAGTGCTATCTACATCCAGACGGTGGAAGGGAGAGAAAGAGAAAGAAGGT ATCCTAGGAATACCTGCCTGCTTAGACCCTCTATAAAAGCTCTGTGCATCCTGCCACTGA GGACTCCGAAGAGGTAGCAGTCTTCTGAAAGACTTCAACTGTGAGGACATGTCTTTCAGA TTTGGCCAACATCTCATCAAGCCCTCTGTAGTGTTTCTCAAAACAGAACTGTCCTTCGCT CTTGTGAATAGGAAACCTGTGGTACCAGGACATGTCCTTGTGTGCCCGCTGCGGCCAGTG GAGCGCTTCCATGACCTGCGTCCTGATGAAGTGGCCGATTTGTTTCAGACGACCCAGAGA GTCGGGACAGTGGTGGAAAAACATTTCCATGGGACCTCTCTCACCTTTTCCATGCAGGAT GGCCCCGAAGCCGGACAGACTGTGAAGCACGTTCACGTCCACGTTCTTCCCAGGAAGGCT GGAGACTTTCACAGGAATGACAGCATCTATGAGGAGCTCCAGAAACATGACAGGNAGGAC TTTCCTGCCTCTTGGAGATCAGAGGAGGAAATGGCAGCAGAAGCCGCAGCTCTGCGGGTC TACTTTCAGTGACACAGGTAAAGACTGCATTTCGTCATTACCCAGAACCCTCCCGTGGCC TCAGGAAAGAGG >OriGene 3' read for NM_002012 unedited
TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TGCAGCAGAAAAGGAAGTTTAATCATAAGGCTGCCGAATAAGGAGACAGGGGGAAACCTC AAATCTGCCTGTCTGAGCCGTTTAGGTCTAGGTATTTTAAGGGAGTTGGAGTGACCGAGG TGGGGGATCACTGGTTGAAAAATACAGGATGGTGAGATGAAAAAACTGCATTTTCATGCT GATTCAGTTCCTCTTGGGGAAAGGCGGGGGGGCGGTCTTCAAACTGGTTGGCAATAGCTC TTTTGCTGGAATTCAGGATCTGAAAAACATCTTAAGCCATTTTTAAACAAACAAAGGCCT TATGATTCTAACATCAAAAATCTTCTCTGTAGGAACAATGAGGAGGCAAATGGTTAGCAT CTGGTGCTCTGGGACTTTTGGTTACCAAGAAGTGGGCCAAAGTGCAGCCTGATTAATGCT TAATTACAACTATATTTCTGTCCAGAACCCAGCATCTAATTCTTGTTAACCCTGTGAGGA TGGTTTCAATAGAATGTTTCCATTGTCCTTAAAGGGAGGTATAGTTAGTCCCATTATACT CCAGGTAATGATGACAGTCTTGGCAAAATCTTAGGACCCTAAGTTGTTTGCCACCTCACA ACCTATATTATCCGTCCTAGTCTCAGTTACCANCATCTGACTGCTGGGAAGACTGAGGGT GATTTGTTTGGTCTGTGCACAAAACCTTTAAAGAGAATACACATGTTAGTTAACTTAAAA ATCCACAGGACTGTGGGCATAGTTTGTGTAACAAGTTAGGGAAATATCTTTCCTGGTAGA TGTACAGGGAAGTCATTTACCTGGAAAACAAGAGGGGTACGCCTTCAGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002012 |
| Insert Size | 2700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002012.1, NP_002003.1 |
| RefSeq Size | 1095 bp |
| RefSeq ORF | 444 bp |
| Locus ID | 2272 |
| UniProt ID | P49789 |
| Domains | HIT |
| Protein Pathways | Non-small cell lung cancer, Purine metabolism, Small cell lung cancer |
| Gene Summary | The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1-4 all encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207120 | FHIT (Myc-DDK-tagged)-Human fragile histidine triad gene (FHIT), transcript variant 1 |
CNY 1,200.00 |
|
| RC207120L3 | Lenti ORF clone of Human fragile histidine triad gene (FHIT), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207120L4 | Lenti ORF clone of Human fragile histidine triad gene (FHIT), transcript variant 1, mGFP tagged |
CNY 3,600.00 |
|
| RG207120 | FHIT (tGFP-tagged) - Human fragile histidine triad gene (FHIT), transcript variant 1 |
CNY 4,370.00 |
|
| SC322660 | FHIT (untagged)-Human fragile histidine triad gene (FHIT), transcript variant 1 |
CNY 1,200.00 |
