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Kir4.1 (KCNJ10) (NM_002241) Human Untagged Clone
CAT＃: SC118741
KCNJ10 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10)
CNY 3,656.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BIRK-10; KCNJ13-PEN; KIR1.2; KIR4.1; SESAME |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118741 sequence for NM_002241 edited (data generated by NextGen Sequencing)
ATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGAAAGCCGGCCCCTA ATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCGCAGCAACGTGAGA ATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTGGACAACCTTCATT GACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGCAGGCACATGGTTC CTCTTTGGCGTGGTGTGGTATCTGGTAGCTGTGGCACATGGGGACCTGCTGGAGCTGGAC CCCCCGGCCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCACTGGAGCCTTCCTC TTCTCCCTTGAATCCCAAACCACCATTGGCTATGGCTTCCGCTACATCAGTGAGGAATGT CCACTGGCCATTGTGCTTCTTATTGCCCAGCTGGTGCTCACCACCATCCTGGAAATCTTC ATCACAGGTACCTTCCTGGCGAAGATTGCCCGGCCCAAGAAGCGGGCTGAGACCATTCGT TTCAGCCAGCATGCAGTTGTGGCCTCCCACAATGGCAAGCCCTGCCTCATGATCCGAGTT GCCAATATGCGCAAAAGCCTCCTCATTGGCTGCCAGGTGACAGGAAAACTGCTTCAGACC CACCAAACCAAGGAAGGGGAGAACATCCGGCTCAACCAGGTCAATGTGACTTTCCAAGTA GACACAGCCTCTGACAGCCCCTTCCTTATTCTACCCCTTACCTTCTATCATGTGGTAGAT GAGACCAGTCCCTTGAAAGATCTCCCTCTTCGCAGTGGTGAGGGTGACTTTGAGCTGGTG CTGATCCTAAGTGGGACAGTGGAGTCCACCAGTGCCACCTGTCAGGTGCGCACTTCCTAC CTGCCAGAGGAGATCCTTTGGGGCTACGAGTTCACACCTGCCATCTCACTGTCAGCCAGT GGTAAATACATAGCTGACTTTAGCCTTTTTGACCAAGTTGTGAAAGTGGCCTCTCCTAGT GGCCTCCGTGACAGCACTGTACGCTACGGAGACCCTGAAAAGCTCAAGTTGGAGGAGTCA TTAAGGGAGCAAGCTGAGAAGGAGGGCAGTGCCCTTAGTGTGCGCATCAGCAATGTCTGA Clone variation with respect to NM_002241.4 >OriGene 5' read for NM_002241 unedited
ACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGCACAGCTCTGCTCCTAACTCC TGCCCGCCCCGTCCGTCCATCTGTCCCGCTGCCCCGCGGCCCATCCAAGGGGCCACTCCA CCTCGGACCCAAGATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGA AAGCCGGCCCCTAATGGGCCCAGGGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCG CAGCAACGTGAGAATGGAGCACATTGCCGACAAGCGCTTCCTCTACCTCAAGGACCTGTG GACAACCTTCATTGACATGCAGTGGCGCTACAAGCTTCTGCTCTTCTCTGCGACCTTTGC AGGCACATGGTTCCTCTTTGGCGTGGTGTGGTATCTGGTAACTGTGGCACATGGGGACCT GCTGGAGCTGGACCCCCCGGGCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCAC TGGAGCCTTGCTCTTGTGCCTTGAATCCAAACCACCATTGGGTATGGGTTACGGTACATA AGTGAGGAATGTGCACTGGACATTGTGCTTGTTATTGAGCAGGTGGTGCTGGCCACCATG CTGAGAATCTAGATGCAAGATCCTTGCTGGGGAAAATTGACCGGACGAAAACGGGAGTGA AACCGTGGTTTGGAAGCGCGTGCGGAGTGGGGACTGTGGCAATGGGTGATGCGTGCGTGT GAAGCAGGTGATGTACTTGGCGGAGACCGGGCTAGGTTGTTGGGTTGATGGACNGATGAA CTATGGTGGTGCGTGGGCCAGGGGTGAGGGGTGGACCCTGTGCTGGAGCCGCGCGCTTGT GAGTGTGTGGGTGGCCCCCGCGGCGAGAGGGGCGGGCTTAAGACCAGGCCAGGAGGNNTG GTGGGGGGNAGCGGGACGGATATAAGCTCGTTACACAACTATGGATAAAAGAACGATATA AAAGATAAGACTAANATATTC >OriGene 3' read for NM_002241 unedited
GTACCGCGGCACGCATTTCTAGTAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTAGG AAACAAAAGGGGCTTTATTTTATTTTATTTATTTTAAAAAGGCGCTACAGTGGCAGGACG CTGCTGGGGATGGGGACACACAGTGTAAGTGGGAGATTTTCCAAGACTTCCCCTCCCTAT TGGCCTCCGAGTTCCTCTCTTCCTGGGCCTATCAGTAGCATAGGATTCAACAAAAGCCTT CATGAATGCCTCTTTATTTCTGAAAAGGAACTTCTACCCCTCCCTTTCCCATTTCCAGAC TTGAAATCCTTCATCAGCTATCTCACTTCCATGTTGAAACCAGGACCACTGATTGTAAGT CTGAAAATCTGGGTGTCCTACCCCATAACCAGGCCAAGATATCTGCCCCCAATGCTAAAG GCTCTGAATATTTCCTTTCTGGACATTCAATATATTTGGGCATATATACATCTATATACA TCCTTCTATATATATATTATCTCTGGTTCTTTCTTCTTAGATAAACCCTTCATATATGGC ATATCCTAATTTCCCAAGCCCTCCCACTCTTTGTTTGCGAGCCTAAGCAAGACTCAAGAG TTTCATTGATTTCACTAAGGTCCCCAGCATCATTGCTTTCTCATCTTTCTACCCCAAGAT TCTTCCCCATCACGTGTTTCCCCCCCTTCCGAAAACCCCCTTTTTAACACCGTGCCTACC GAAATTCCCCTTTTTAAACTGGGCCTCTACTCTGCTGATTTCCAAGCCCCAGTGGGTTGG GGGCCCATTTCTAACAAACCTGGCGGGTTCTAAGCCGGCCTATGGCTAAAGAAGATTAGG GCCAAGGGAAAAACATTGTCCAAAAACCCTTTAAGTAAGGAAGGGGGACTTGCCCCCGCG CAGGGCCTCGGGGAAAAAACCCATCCCCTTTCAGGACACGGGTTTAAGTGCCCCTTAACG AACCCGGGGAAACCGCAGGATCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002241 |
| Insert Size | 4600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002241.2, NP_002232.2 |
| RefSeq Size | 4515 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 3766 |
| UniProt ID | P78508 |
| Domains | IRK |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| Gene Summary | This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Superresolution Imaging of Aquaporin-4 Cluster Size in Antibody-Stained Paraffin Brain Sections
,Smith, AJ;Verkman, AS;,
Biophys. J.
,PubMed ID 26682810
[KCNJ10]
|
|
Development of a Selective Small-Molecule Inhibitor of Kir1.1, the Renal Outer Medullary Potassium Channel
,Gautam Bhave, Brian A. Chauder, Wen Liu, Eric S. Dawson, Rishin Kadakia, Thuy T. Nguyen, L. Michelle Lewis, Jens Meiler, C. David Weaver, Lisa M. Satlin, Craig W. Lindsley, and Jerod S. Denton,
Mol. Pharmacol., Jan 2011; 79: 42 - 50
[KCNJ10]
|
|
High-throughput screening reveals a small-molecule inhibitor of ROMK and Kir7.1
,L. Michelle Lewis, Gautam Bhave, Brian A. Chauder, Sreedatta Banerjee, Katherina A. Lornsen, Rey Redha, Katherine Fallen, Craig W. Lindsley, C. David Weaver, and Jerod S. Denton,
Mol. Pharmacol., Aug 2009; 10.1124/mol.109.059840.
[KCNJ10]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213543 | KCNJ10 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10) |
CNY 3,656.00 |
|
| RC213543L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC213543L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), mGFP tagged |
CNY 5,890.00 |
|
| RC213543L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC213543L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), mGFP tagged |
CNY 6,056.00 |
|
| RG213543 | KCNJ10 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10) |
CNY 5,256.00 |
