Kir4.1 (KCNJ10) (NM_002241) Human Tagged ORF Clone
CAT#: RC213543
KCNJ10 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10)
ORF Plasmid: tGFP
"NM_002241" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BIRK-10; KCNJ13-PEN; KIR1.2; KIR4.1; SESAME |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213543 representing NM_002241
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCAGTTGCCAAGGTGTATTACAGTCAGACCACTCAGACAGAAAGCCGGCCCCTAATGGGCCCAG GGATACGACGGCGGAGAGTCCTGACAAAAGATGGTCGCAGCAACGTGAGAATGGAGCACATTGCCGACAA GCGCTTCCTCTACCTCAAGGACCTGTGGACAACCTTCATTGACATGCAGTGGCGCTACAAGCTTCTGCTC TTCTCTGCGACCTTTGCAGGCACATGGTTCCTCTTTGGCGTGGTGTGGTATCTGGTAGCTGTGGCACATG GGGACCTGCTGGAGCTGGACCCCCCGGCCAACCACACCCCCTGTGTGGTACAGGTGCACACACTCACTGG AGCCTTCCTCTTCTCCCTTGAATCCCAAACCACCATTGGCTATGGCTTCCGCTACATCAGTGAGGAATGT CCACTGGCCATTGTGCTTCTTATTGCCCAGCTGGTGCTCACCACCATCCTGGAAATCTTCATCACAGGTA CCTTCCTGGCGAAGATTGCCCGGCCCAAGAAGCGGGCTGAGACCATTCGTTTCAGCCAGCATGCAGTTGT GGCCTCCCACAATGGCAAGCCCTGCCTCATGATCCGAGTTGCCAATATGCGCAAAAGCCTCCTCATTGGC TGCCAGGTGACAGGAAAACTGCTTCAGACCCACCAAACCAAGGAAGGGGAGAACATCCGGCTCAACCAGG TCAATGTGACTTTCCAAGTAGACACAGCCTCTGACAGCCCCTTCCTTATTCTACCCCTTACCTTCTATCA TGTGGTAGATGAGACCAGTCCCTTGAAAGATCTCCCTCTTCGCAGTGGTGAGGGTGACTTTGAGCTGGTG CTGATCCTAAGTGGGACAGTGGAGTCCACCAGTGCCACCTGTCAGGTGCGCACTTCCTACCTGCCAGAGG AGATCCTTTGGGGCTACGAGTTCACACCTGCCATCTCACTGTCAGCCAGTGGTAAATACATAGCTGACTT TAGCCTTTTTGACCAAGTTGTGAAAGTGGCCTCTCCTAGTGGCCTCCGTGACAGCACTGTACGCTACGGA GACCCTGAAAAGCTCAAGTTGGAGGAGTCATTAAGGGAGCAAGCTGAGAAGGAGGGCAGTGCCCTTAGTG TGCGCATCAGCAATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213543 representing NM_002241
Red=Cloning site Green=Tags(s) MTSVAKVYYSQTTQTESRPLMGPGIRRRRVLTKDGRSNVRMEHIADKRFLYLKDLWTTFIDMQWRYKLLL FSATFAGTWFLFGVVWYLVAVAHGDLLELDPPANHTPCVVQVHTLTGAFLFSLESQTTIGYGFRYISEEC PLAIVLLIAQLVLTTILEIFITGTFLAKIARPKKRAETIRFSQHAVVASHNGKPCLMIRVANMRKSLLIG CQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELV LILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYIADFSLFDQVVKVASPSGLRDSTVRYG DPEKLKLEESLREQAEKEGSALSVRISNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002241 |
ORF Size | 1137 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002241.5 |
RefSeq Size | 4515 bp |
RefSeq ORF | 1140 bp |
Locus ID | 3766 |
UniProt ID | P78508 |
Domains | IRK |
Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
MW | 42.3 kDa |
Gene Summary | This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Modified HEK cells simulate DCT cells in their sensitivity and response to changes in extracellular K
,Murthy, M;O'Shaughnessy, KM;,
Physiol Rep
,PubMed ID 31762176
[Kir4.1]
|
Development and Validation of Fluorescence-Based and Automated Patch Clamp-Based Functional Assays for the Inward Rectifier Potassium Channel Kir4.1
,Raphemot, RR;Kadakia, RR;Olsen, ML;Banerjee, S;Days, E;Smith, SS;Weaver, CD;Denton, JS;,
Assay Drug Dev Technol
,PubMed ID 24266659
[Kir4.1]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213543L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC213543L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), mGFP tagged |
CNY 5,890.00 |
|
RC213543L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC213543L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10), mGFP tagged |
CNY 6,056.00 |
|
RG213543 | KCNJ10 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10) |
CNY 5,256.00 |
|
SC118741 | KCNJ10 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 10 (KCNJ10) |
CNY 3,656.00 |