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MIF (NM_002415) Human Untagged Clone
CAT＃: SC118641
MIF (untagged)-Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF)
CNY 1,200.00
CNY 2,950.00
| Cited in 4 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GIF; GLIF; MMIF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118641 sequence for NM_002415 edited (data generated by NextGen Sequencing)
ATGCCGATGTTCATCGTAAACACCAACGTGCCCCGCGCCTCCGTGCCGGACGGGTTCCTC TCCGAGCTCACCCAGCAGCTGGCGCAGGCCACCGGCAAGCCCCCCCAGTACATCGCGGTG CACGTGGTCCCGGACCAGCTCATGGCCTTCGGCGGCTCCAGCGAGCCGTGCGCGCTCTGC AGCCTGCACAGCATCGGCAAGATCGGCGGCGCGCAGAACCGCTCCTACAGCAAGCTGCTG TGCGGCCTGCTGGCCGAGCGCCTGCGCATCAGCCCGGACAGGGTCTACATCAACTATTAC GACATGAACGCGGCCAATGTGGGCTGGAACAACTCCACCTTCGCCTAA Clone variation with respect to NM_002415.1 >OriGene 5' read for NM_002415 unedited
GTCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGCGC GTGCGTCTGTGCCTCTGCGCGGGTCTCCTGGTCCTTCTGCCATCATGCCGATGTTCATCG TAAACACCAACGTGCCCCGCGCCTCCGTGCCGGACGGGTTCCTCTCCGAGCTCACCCAGC AGCTGGCGCAGGCCACCGGCAAGCCCCCCCAGTACATCGCGGTGCACGTGGTCCCGGACC AGCTCATGGCCTTCGGCGGCTCCAGCGAGCCGTGCGCGCTCTGCAGCCTGCACAGCATCG GCAAGATCGGCGGCGCGCAGAACCGCTCCTACAGCAAGCTGCTGTGCGGCCTGCTGGCCG AGCGCCTGCGCATCAGCCCGGACAGGGTCTACATCAACTATTACGACATGAACGCGGCCA ATGTGGGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTCTGCGC TGGCTCCACCCGGGAACCCGCCGCACGCTGTGTTCTAGGCCCGCCCACCCCAACCTTCTG GTGGGGAGAAATAAACGGTTTAGAGACTAAAAAAAAAAAAAAAAAACTCGACTCTAGATT GCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCC CCAGTGCCTCTCCTGGCCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCCTTGTCCTAA TAAAATTAAGCTGCATCATTTTGCCTGACCAGGTGTCCCTTCATAATATTATGGGTGGCA GGGGGTTGCTATGGACCAAGGGGCAAGTTGGGAAGAACACCTGTAGGCCCGCCGGGTCTA TTGGGACCCAACCGGGAGGCCAGGGACCAATCTGGCTCACTGGAATCCCGCCTCCTGAGT CAAGCGAATCTCCTGCCT >OriGene 3' read for NM_002415 unedited
ACTTCCAGGGCCAGGAAGCACTGGGGAGGGTCACAGGGATGCCACCCGGGATCTGTCAGA AACAGCTATGACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGTCTCT AAACCGTTTATTTCTCCCCACCAGAAGGTTGGGGTGGGCGGGCCTAGAACACAGCGTGCG GCGGGTTCCCGGGTGGAGCCAGCGCAGACAGCGTGGGTCCCTGCGGCTCTTAGGCGAAGG TGGAGTTGTTCCAGCCCACATTGGCCGCGTTCATGTCGTAATAGTTGATGTAGACCCTGT CCGGGCTGATGCGCAGGCGCTCGGCCAGCAGGCCGCACAGCAGCTTGCTGTAAGAGCGGT TCTGCGCGCCGCCGATCTTGCCGATGCTGTGCAGGCTGCAGAGCGCGCACGGCTCGNNNG AGCCGCCGAAGGCCATGAGCTGGTCCGGGACCACGTGCACCGCGATGTACTGGGGGGGCT TGCCGGTGGCCTGCGCCAGCTGCTGNGTGAGCTCGGAAGGAACCCGTCCGGCACGGAGGC GCGGGGCACGTTGGTGTTTACGATGAACATCGGCATGATGGCAGAAGGACCAGGAGACCC GCGCAGAGGCACAGACGCACGCGCCGNCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGA GTCGTATTCAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTTATAGNACCTCCACCGT ACACGCCTACCGCCCATNNNNCCAACGGGCCGGGTTATTACGACATTNTGGAAAGTNCCG TTGATTTTTGTGCCAAACAAACTCTCATTGACGTCAATGGGGTGGGAGACTTGNAAATTC CCGGAGTTCAACCGCTATTCCGCCCATTGGTGTTCTGCCAAAACGNCACACCATGGNATA GCGATGACTAATCGTANNAGTACTGCCAGTAGNAAAGTCCCGAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002415 |
| Insert Size | 560 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002415.1, NP_002406.1 |
| RefSeq Size | 561 bp |
| RefSeq ORF | 348 bp |
| Locus ID | 4282 |
| UniProt ID | P14174 |
| Domains | MIF |
| Protein Families | Druggable Genome |
| Protein Pathways | Phenylalanine metabolism, Tyrosine metabolism |
| Gene Summary | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
microRNA-1228(⁎) impairs the pro-angiogenic activity of gastric cancer cells by targeting macrophage migration inhibitory factor
,Jia, L;Chen, J;Xie, C;Shao, L;Xu, Z;Zhang, L;,
Life Sci.
,PubMed ID 28465246
[MIF]
|
|
Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells
,Sarkar, S;Siddiqui, AA;Mazumder, S;De, R;Saha, SJ;Banerjee, C;Iqbal, MS;Adhikari, S;Alam, A;Roy, S;Bandyopadhyay, U;,
J. Agric. Food Chem.
,PubMed ID 25929447
[MIF]
|
|
Macrophage Migration Inhibitory Factor Regulates Akt Signaling in Hypoxic Culture to Modulate Senescence of Human Mesenchymal Stem Cells
,Palumbo, SL;Tsai, TL;Li, WJ;,
Stem Cells Dev.
,PubMed ID 24274936
[MIF]
|
|
Novel Anti-inflammatory Activity of Epoxyazadiradione against Macrophage Migration Inhibitory Factor: INHIBITION OF TAUTOMERASE AND PROINFLAMMATORY ACTIVITIES OF MACROPHAGE MIGRATION INHIBITORY FACTOR
,Athar Alam, Saikat Haldar, Hirekodathakallu V. Thulasiram, Rahul Kumar, Manish Goyal, Mohd Shameel Iqbal, Chinmay Pal, Sumanta Dey, Samik Bindu, Souvik Sarkar, Uttam Pal, Nakul C. Maiti, and Uday Bandyopadhyay,
J. Biol. Chem., Jul 2012; 287: 24844 - 24861.
[MIF]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205106 | MIF (Myc-DDK-tagged)-Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF) |
CNY 1,200.00 |
|
| RC205106L1 | Lenti ORF clone of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC205106L2 | Lenti ORF clone of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), mGFP tagged |
CNY 5,890.00 |
|
| RC205106L3 | Lenti ORF clone of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC205106L4 | Lenti ORF clone of Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), mGFP tagged |
CNY 3,600.00 |
|
| RG205106 | MIF (tGFP-tagged) - Human macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF) |
CNY 2,800.00 |
