SELENOW (NM_003009) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Synonyms	selW; SEPW1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC118238 sequence for NM_003009 edited (data generated by NextGen Sequencing) ATGGCTCTCGCCGTCCGAGTCGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTT CAGCTCAAGAAGAAGTTAGAAGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGA ACTCCCCAGGCCACCGGGTTCTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAG AAGAAAGGCGATGGCTACGTGGACACAGAAAGCAAGTTTCTGAAGTTGGTGGCCGCCATC AAAGCCGCCTTGGCTCAGGGCTAA Clone variation with respect to NM_003009.2 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_003009 unedited GGATTTTGTATACGACTCACTATAGGCGGCCGCGNATTCGGCACGAGGGGTTAGTGTGGC CCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGAGCCATGGCTCTCGCCGTCCGAGT CGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTTCAGCTCAAGAAGAAGTTAGA AGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGAACTCCCCAGGCCACCGGGTT CTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAGAAGAAAGGCGATGGCTACGT GGACACAGAAAGCAAGTTTCTGAAGTTGGTGGCCGCCATCAAAGCCGCCTTGGCTCAGGG CTAATGCGCCCTGAAGGCAGAGTCCAGGGACCTTGACCCAGCCCCTCTCAGCAGACGCTT CATGATAGGAAGGACTGAAAAGTCTTGTGGACACCTGGTCTTTCCCTGATGTTCTCGTGG CTGCTGTTGGGGGCAGAGATTGACGCCCCCGGTCTTTGCCTCTGAGCGGGAGAGTCTGTG TGTATGTGTCTTCCCCGGAATCCACACCACCCCACCCTCCTCCTGTCCCGTGGTTTCATC ATATCTCTTTGCATACCCCATGTCTTCCCCAGTTGTCCCCTGGAGTTTGGGGGGACATCC CGCCTCAGGCATCCTTCTCAGGGGAAGCCAAGAGAGGGCATCAGATGGGTGGGTTTCTGA TTGTGGNCACGTTTGNCACCGTTCACGATTCAATAATATTGGATGAATTTAACCAAAAAA AAAAAAAAAACTCGCTCTAGATTGCGGCCGCGTCATTAGCTGTTCTGACAGATCCCGGTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_003009 unedited AATTCTGTCCGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTGGTTAAATTCA TCCAATATTTATTGAATCGTGAACGCTTGCAAACGTTGCCACAATCAGAAACCCACCCAT CCTGATGCCTCTCTTGGCTTCCCCTTTTGAAGGATGCCTGAGGCGGGATGTCCCCCCAAA CTCCCTGGGACAACTGGGGAAGACATGGGGTATGCAAAGAGATATGATGAAACCACGGGA CAGGAGGAGGGTGGGGTGGTGTGGATTCCGGGGAAGACACATACACACAGACTCTCCCGC TCAGAGGCTAAGACCGGGGGCGTCAATCTCTGCCCCCAACAGCAGCCACGAGAACACCAG GGAAAGACCAGGTGTCCACAAGACTTTTCAGTCCTTTCTATCATGAAGCGCCTGCTGAGA GGGGCTGGGTCAAGGTCCCTGGACTCTGCCTTCAGGGCGCATTAGCCCTGAGCCAAGGCG GCTTTGATGGCGGCCACCAACTTCAGAAACTTGCTTTCTGTGTCCACGTAGCCCATCGCC TTTTTTCTTAGAGTGAATCAACTTCCCGGCTACCATCACTTCAAAGAACCCGGTGGCCTC GGGAGTTCCCTCCCCGCAAATGTCCAGCCGGCCCGGGAACCCCATCTTCTAACTTCTTCT TGAGCCGAAGATACTTGGCATTGTACCCTCCAGTGCCACCACTAAACCACTCGGCCGGCG AGAGCCCTGGTTTCGGGGGTGCCCCATCCGCTGGAGAAGGGGACGTCCCGGCCCAAACTA ACTCCTCGTGCCCAATTCCCGGGCCGCCCTAAAACGAGGTCCTATTCCAAAATTTTGAGG GATACCCAAACGACCTCTGTTCATCAGAACCCCCAACGTTACAGCTTCCGGGCAATTTGC GCAACGGGCGCGTGTTTTTCCCCCCCN
Restriction Sites	NotI-NotI
ACCN	NM_003009
Insert Size	800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). The expression of this clone is not guaranteed due to the nature of selenoproteins.
OTI Annotation	This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003009.2, NP_003000.1
RefSeq Size	893 bp
Locus ID	6415
UniProt ID	P63302
Gene Summary	This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle, heart and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A retroprocessed pseudogene of this locus has been identified on chromosome 1. [provided by RefSeq, Aug 2017]

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