United States
Germany
Japan
United Kingdom
China
SELENOW (NM_003009) Human Tagged ORF Clone
CAT＃: RG208062
- TrueORF®
SEPW1 (GFP-tagged) - Human selenoprotein W, 1 (SEPW1), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
ORF Plasmid: DDK
"NM_003009" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 2,544.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged ORF Clone |
| Synonyms | selW; SEPW1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208062 representing NM_003009
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTCGCCGTCCGAGTCGTTTATTGTGGCGCTTGAGGCTACAAGTCCAAGTATCTTCAGCTCAAGA AGAAGTTAGAAGATGAGTTCCCCGGCCGCCTGGACATCTGCGGCGAGGGAACTCCCCAGGCCACCGGGTT CTTTGAAGTGATGGTAGCCGGGAAGTTGATTCACTCTAAGAAGAAAGGCGATGGCTACGTGGACACAGAA AGCAAGTTTCTGAAGTTGGTGGCCGCCATCAAAGCCGCCTTGGCTCAGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208062 representing NM_003009
Red=Cloning site Green=Tags(s) MALAVRVVYCGA*GYKSKYLQLKKKLEDEFPGRLDICGEGTPQATGFFEVMVAGKLIHSKKKGDGYVDTE SKFLKLVAAIKAALAQG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003009 |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins. |
| OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003009.4 |
| RefSeq Size | 893 bp |
| RefSeq ORF | 264 bp |
| Locus ID | 6415 |
| UniProt ID | P63302 |
| Gene Summary | This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle, heart and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A retroprocessed pseudogene of this locus has been identified on chromosome 1. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208062 | SEPW1 (Myc-DDK-tagged)-Human selenoprotein W, 1 (SEPW1), (Note, selenocysteine protein, internal stop codon, see reference data summary) |
CNY 998.00 |
|
| SC118238 | SEPW1 (untagged)-Human selenoprotein W, 1 (SEPW1) (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) |
CNY 3,432.00 |
其它SELENOW产品
