United States
Germany
Japan
United Kingdom
China
SUPT5H (NM_003169) Human Untagged Clone
CAT＃: SC118151
SUPT5H (untagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1
CNY 7,576.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPT5; SPT5H; Tat-CT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC118151 sequence for NM_003169 edited (data generated by NextGen Sequencing)
ATGTCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGACGGCGAGGAGG CCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAAGAGCCTGAGGACGAAGAGGA GGAGGAAGAGGAGGAGGAATACGATGAGGAAGAGGAGGAAGAAGATGATGACCGACCCCCCAAGAAACCC CGCCATGGAGGCTTCATTCTGGACGAGGCTGATGTTGACGATGAGTATGAGGACGAGGACCAGTGGGAGG ATGGAGCAGAGGACATTCTAGAGAAAGAAGAGATTGAAGCCTCCAATATCGATAATGTTGTCCTGGATGA AGATCGTTCTGGGGCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTAT TACATGAAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAGCTCTCAGACG ACATCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGGACTGTCAAATGTAAGATTGG GGAGGAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTCATTGCCTACCAGTTCACAGACACGCCCCTG CAGATCAAGTCAGTAGTGGCACCAGAGCATGTGAAGGGCTACATCTACGTGGAGGCCTACAAGCAGACCC ACGTGAAGCAGGCCATTGAGGGGGTGGGCAACCTGCGGCTTGGCTACTGGAACCAGCAGATGGTGCCCAT CAAGGAGATGACAGACGTGCTCAAAGTGGTGAAGGAGGTGGCCAACCTGAAACCAAAGTCCTGGGTCCGC CTCAAGCGGGGCATCTACAAGGATGACATTGCTCAGGTGGACTACGTGGAGCCCAGCCAGAACACCATCT CCCTGAAGATGATCCCACGCATCGACTACGATCGCATCAAGGCCCGCATGAGCTTGAAAGACTGGTTTGC CAAAAGGAAGAAGTTTAAGCGGCCTCCACAGAGGCTGTTTGATGCTGAGAAGATCAGGTCCCTGGGGGGT GATGTTGCCTCTGATGGTGACTTCCTCATCTTTGAGGGGAACCGTTACAGCCGGAAGGGCTTTCTGTTCA AGAGCTTCGCCATGTCTGCTGTGATCACGGAGGGTGTGAAGCCAACACTCTCTGAGCTGGAAAAGTTTGA GGACCAGCCAGAGGGCATTGACCTGGAGGTGGTGACTGAGAGCACAGGGAAGGAGCGGGAGCACAACTTC CAACCTGGGGACAACGTGGAGGTCTGTGAGGGTGAGCTCATCAACCTGCAGGGCAAGATCCTCAGCGTGG ATGGCAACAAGATCACCATCATGCCCAAGCATGAGGACCTCAAGGACATGTTGGAGTTCCCAGCCCAGGA ACTTAGAAAATACTTCAAGATGGGGGACCACGTGAAGGTGATTGCTGGCCGATTCGAGGGCGACACAGGC CTCATTGTGCGGGTGGAGGAGAATTTCGTTATCCTGTTCTCTGACCTCACCATGCATGAGCTGAAGGTGC TCCCCCGGGACCTGCAGCTCTGCTCAGAGACAGCATCAGGTGTGGATGTTGGGGGCCAGCATGAATGGGG CGAGCTGGTGCAGCTGGATCCCCAGACTGTGGGTGTCATCGTGCGACTAGAACGGGAGACCTTCCAGGTG CTGAACATGTACGGGAAGGTGGTGACTGTCAGACATCAGGCTGTGACCCGGAAGAAGGACAACCGCTTTG CTGTGGCCTTGGACTCAGAGCAGAACAACATCCATGTGAAAGACATCGTTAAGGTCATTGATGGCCCCCA CTCAGGCCGAGAAGGGGAGATTCGCCATCTCTTCCGAAGCTTCGCCTTCCTACATTGCAAGAAACTGGTG GAGAACGGGGGCATGTTTGTCTGCAAGACCCGCCACCTGGTGCTGGCTGGGGGCTCAAAGCCCCGTGATG TGACCAACTTCACCGTGGGTGGCTTTGCGCCTATGAGTCCCCGGATCAGCAGCCCCATGCACCCCAGTGC TGGAGGTCAGCGTGGCGGCTTTGGTAGCCCAGGTGGCGGCAGTGGTGGCATGAGCAGGGGCCGGGGCCGG AGGGACAACGAACTCATCGGCCAGACCGTGCGCATCTCCCAGGGGCCCTACAAAGGCTACATCGGTGTGG TGAAAGATGCCACAGAGTCCACGGCCCGTGTGGAGCTGCACTCCACCTGCCAGACCATCTCTGTGGACCG TCAGCGGCTCACCACGGTGGGCTCACGGCGCCCGGGCGGCATGACCTCGACCTATGGGAGGACGCCCATG TATGGCTCCCAGACGCCCATGTATGGCTCTGGCTCCCGAACACCCATGTACGGCTCACAGACACCCCTCC AGGATGGTAGCCGCACCCCACACTACGGCTCACAGACGCCCCTGCATGATGGCAGCCGCACTCCTGCCCA GAGTGGGGCCTGGGACCCCAACAACCCCAACACGCCGTCACGGGCTGAGGAAGAATATGAGTATGCTTTC GATGATGAGCCCACCCCGTCCCCGCAGGCCTATGGGGGAACCCCCAATCCCCAAACACCTGGCTACCCAG ACCCCTCGTCCCCACAGGTCAACCCACAATACAACCCGCAGACGCCAGGGACGCCGGCCATGTACAACAC AGACCAGTTCTCTCCCTATGCTGCCCCCTCCCCACAAGGTTCCTACCAGCCCAGCCCCAGCCCCCAGAGC TACCACCAGGTGGCGCCAAGCCCAGCAGGCTACCAGAATACCCACTCCCCAGCCAGCTACCACCCTACAC CGTCGCCCATGGCCTATCAGGCTAGCCCCAGCCCGAGCCCCGTTGGCTACAGTCCTATGACACCTGGAGC TCCCTCCCCTGGTGGCTACAACCCACACACGCCAGGCTCAGGCATCGAGCAGAACTCCAGCGACTGGGTA ACCACTGACATTCAGGTGAAGGTGCGGGACACCTACCTGGATACACAGGTGGTGGGACAGACAGGTGTCA TCCGCAGTGTCACGGGGGGCATGTGCTCTGTGTACCTGAAGGACAGTGAGAAGGTTGTCAGCATTTCCAG TGAGCACCTGGAGCCTATCACCCCCACCAAGAACAACAAGGTGAAAGTGATCCTGGGCGAGGATCGGGAA GCCACGGGCGTCCTACTGAGCATTGATGGTGAGGATGGCATTGTCCGTATGGACCTTGATGAGCAGCTCA AGATCCTCAACCTCCGCTTCCTGGGGAAGCTCCTGGAAGCCTGA Clone variation with respect to NM_003169.3 >OriGene 5' read for NM_003169 unedited
GTTACCATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGAACAGCAGCT GGTACCGAAGGCGGAGGTGGAGCCCGAGAGGGAACCAGCGGGGAAACTGAGGCTCGGGGT GGAGCGCAGGATTGTGGGACGCGCCAAGGCTGCTGTCTTTCCCAGCAGCAGCGGAAGATG TCGGACAGCGAGGACAGCAACTTTTCCGAGGAGGAGGACAGCGAGCGCAGCAGTGACGGC GAGGAGGCCGAGGTAGACGAAGAGCGGCGGAGTGCAGCGGGCAGTGAGAAAGAAGAAGAG CCTGAGGACGAAGAGGAGGAGGAAGAGGAGGAGGAATACGATGAGGAAGAGGAGGAAGAA GATGATGACCGACCCCCCAAGAAACCCCGCCATGGAGGCTTCATTCTGGACGAGGCTGAT GTTGACGATGAGTATGAGGACGAGGACCAGTGGGAGGATGGAGCAGAGGACATTCTAGAG AAAGAAGAGATTGAAGCCTCCAATATCGATAATGTTGTCCTGGATGAAGATCGTTCTGGG GCTCGCCGCCTGCAAAACCTCTGGAGGGACCAGCGAGAAGAAGAACTGGGCGAGTATTAC ATGAAGAAATACGCCAAGTCATCTGTGGGAGAGACGGTGTATGGAGGATCTGATGAGCTC TCAGACGACATCACCCAGCAGCAGCTGCTCCCAGGAGTCAAGGATCCCAATCTGTGGACT GTCAAATGTAAGATTGGGGAGGAACGGGCCACGGCCATTTCCTTGATGCGCAAGTTCATT GCCTACCAGTTCACAGACACGCCCCTGCAGATCAAGTCAGTAGTGGCACCAGAGCATGTG AAGGCCTACATCTACGTGGAAGCCTACAGCAGACCCACGTGAGCAGCCATTGAGGGGTTG GCAACCTGCCGCTTGCTACTGGAACAGCA >OriGene 3' read for NM_003169 unedited
GCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTAAACCAAACAACTTTTTTTTATTGAAAAACGGGACAACAAAAGC AAGCTGTGGGGAGGAGGTACAGGAAGGTGGGGACCCTCCCCCAAACTTTACTTAAATTCC AATGAGCCCCAGGGGGGGAAAAAATGGAAAACTTAAAGGAAAAACAAAAGGAAATCCAAA ACAATCCCCCTAACCCTGCAGGAGGATCTTGTGTCACAGCCAAGGGCCAGGGAAGGAAGG AGGATCACTTTTTATTCGACAAAATCCACCGGCCCTGCCTGCTTTAAGGTTTCAAGAACT TCCCCAAGAAACGGAGGTTGAGGATCTTGAACTGCTCATTAAGGGCCATACGGACAATGC CATCCTCACCATTAATGCTCAATAAGACCCCCGTGGCTTCCCGATCCTTGCCCAAGATCA CTTTCACCTTGTTGTTCTTGGGGGGGGTGATAGGCTCCAAGTGCTCACTGGAAATGCTGA CAACCTTTTCACTGTCCTTTAAGTACACAAAGCACATGCCCCCCGTGACACTGGGGATGA CACCTGTCTGTCCCACCACCTGTGTATCCAGGTAGGTGTCCCGCACCTTCACCTGAATGT CAGTGGTTACCCANTCGCTGGAGTTCTGCTCCATGCCTGACCCTGGCGTGTGTGGGTTGT ANCCACCAAGGGAAGGAGCTCCAGTGTCATAAGACTGTATCCAACGGGGCTCGGGCTGGG GCTACCCTGATAGGCCATGGGCCACAGTGTAGGGTGGTAACCTGGTGGGGATTGGGTATT CTTGTACCCTGCTGGGCTGGCCGCCCCTGTGGGTACCTTGGGGGCTGGGCTGGGCTGTAG AAACCTTGTGGGAAGGGGGCCACTAGGGAAAAACTGGTTGGTTTTAATGGCCCGGTTCCT GCGTTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003169 |
| Insert Size | 4100 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003169.2, NP_003160.2 |
| RefSeq Size | 3798 bp |
| RefSeq ORF | 3264 bp |
| Locus ID | 6829 |
| UniProt ID | O00267 |
| Domains | Supt5, KOW, NGN, KOW |
| Protein Families | Transcription Factors |
| Gene Summary | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Variants 1, 2, 3, and 5 all encode the same isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201196 | SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 |
CNY 7,568.00 |
|
| RC201196L1 | Lenti-ORF clone of SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 |
CNY 9,968.00 |
|
| RC201196L2 | Lenti-ORF clone of SUPT5H (mGFP-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 |
CNY 7,600.00 |
|
| RC201196L3 | Lenti-ORF clone of SUPT5H (Myc-DDK-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 |
CNY 7,600.00 |
|
| RC201196L4 | Lenti-ORF clone of SUPT5H (mGFP-tagged)-Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 |
CNY 7,600.00 |
|
| RG201196 | SUPT5H (tGFP-tagged) - Human suppressor of Ty 5 homolog (S. cerevisiae) (SUPT5H), transcript variant 1 |
CNY 6,270.00 |
