DIS3 (NM_014953) Human Untagged Clone

CAT#: SC114689

DIS3 (untagged)-Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 1



  "NM_014953" in other vectors (6)

CNY 10,536.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DIS3 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms 2810028N01Rik; dis3p; EXOSC11; KIAA1008; RRP44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_014953, the custom clone sequence may differ by one or more nucleotides


ATGCTCAAGTCCAAGACGTTCTTAAAAAAGACCCGGGCGGGCGGCGTGATGAAGATCGTGCGCGAGCACT
ACCTGCGAGACGACATCGGCTGCGGTGCGCCCGGGTGCGCAGCGTGTGGAGGGGCGCACGAGGGGCCGGC
CCTGGAGCCGCAGCCCCAGGACCCGGCGAGCAGCGTCTGCCCGCAACCGCACTACTTGCTGCCCGACACT
AATGTGTTACTGCACCAGATTGATGTTCTTGAGGACCCTGCCATCAGGAATGTAATTGTGCTACAAACAG
TTCTTCAAGAAGTGAGAAATCGCAGTGCCCCCGTATATAAACGCATCCGAGATGTGACTAATAACCAAGA
GAAGCATTTCTATACTTTCACTAATGAGCACCATAGAGAAACCTATGTAGAACAAGAACAGGGAGAAAAT
GCTAATGACAGGAATGATAGAGCGATTCGAGTAGCAGCAAAATGGTACAATGAACATTTGAAAAAAATGT
CAGCAGACAACCAGCTGCAAGTTATCTTCATAACAAATGACAGGAGAAACAAAGAGAAAGCCATAGAAGA
AGGAATACCAGCTTTCACTTGTGAAGAATATGTAAAGAGCCTAACTGCTAACCCCGAACTCATAGATCGT
CTTGCTTGTTTGTCTGAAGAAGGGAATGAAATAGAAAGTGGAAAAATAATATTTTCAGAGCATCTTCCCT
TAAGTAAGCTACAGCAAGGCATAAAATCTGGTACATACCTTCAAGGAACATTTAGAGCTAGCAGGGAAAA
TTACTTGGAAGCTACAGTATGGATTCATGGCGACAATGAAGAAAATAAAGAGATAATCTTACAGGGACTT
AAACATTTAAACAGAGCTGTTCACGAAGATATTGTGGCTGTGGAGCTTCTCCCCAAGAGTCAGTGGGTAG
CACCATCTTCTGTGGTTTTACATGATGAAGGTCAAAATGAAGAAGATGTGGAGAAAGAAGAAGAGACAGA
ACGAATGCTTAAGACTGCTGTAAGCGAGAAAATGTTGAAGCCTACAGGTAGAGTTGTAGGAATAATAAAA
AGGAATTGGAGACCATATTGTGGCATGCTTTCCAAGTCTGACATTAAGGAGTCAAGAAGACATCTCTTTA
CACCTGCTGATAAGAGAATCCCTCGAATTCGCATAGAAACCAGACAGGCTTCCACATTAGAAGGACGGAG
AATTATTGTTGCTATTGATGGTTGGCCCAGAAATTCCAGATATCCAAATGGACACTTTGTGAGAAATTTA
GGTGATGTTGGAGAGAAAGAGACTGAAACAGAAGTTTTGTTACTTGAACACGATGTTCCCCATCAGCCTT
TTTCACAGGCTGTTCTTAGTTTTCTGCCAAAGATGCCCTGGAGCATTACTGAAAAGGACATGAAAAACCG
AGAAGACCTGAGGCATCTGTGTATTTGTAGTGTAGACCCACCAGGATGTACTGATATAGACGATGCTCTA
CATTGTCGAGAACTCGAAAATGGAAATTTGGAGGTTGGTGTTCATATTGCTGATGTGAGCCATTTTATTA
GGCCAGGAAATGCCTTGGATCAAGAATCAGCCAGAAGAGGAACAACTGTGTATCTTTGTGAAAAGAGGAT
TGACATGGTTCCAGAGTTGCTTAGCTCTAACTTGTGTTCCTTAAAATGTGACGTGGACAGGCTGGCATTT
TCATGTATTTGGGAAATGAATCACAATGCTGAAATCTTAAAAACGAAGTTTACCAAAAGTGTTATTAATT
CAAAGGCATCTCTGACGTATGCTGAAGCTCAGTTGAGAATTGATTCAGCAAACATGAATGATGATATTAC
CACTAGTCTCCGTGGACTGAATAAACTAGCCAAAATTCTGAAGAAAAGAAGGATTGAAAAAGGGGCTTTG
ACTCTATCCTCTCCTGAAGTTCGATTCCACATGGACAGTGAAACTCACGATCCTATAGATCTGCAGACCA
AGGAACTTAGGGAAACAAATTCCATGGTTGAAGAATTTATGTTACTTGCCAATATTTCTGTTGCAAAAAA
AATTCATGAGGAATTTTCTGAACATGCTCTGCTTCGAAAACATCCTGCTCCACCTCCATCAAATTATGAA
ATTCTTGTTAAGGCAGCCAGGTCAAGGAATTTGGAAATTAAGACTGATACAGCCAAGTCTTTGGCTGAGT
CTTTGGATCAGGCCGAATCTCCTACTTTTCCATATCTAAACACTCTGTTGAGAATATTAGCCACTCGCTG
TATGATGCAAGCTGTGTACTTCTGTTCTGGAATGGATAATGATTTTCATCACTATGGCTTAGCGTCTCCA
ATATACACACATTTTACTTCACCCATTAGAAGATACGCAGATGTCATTGTTCATCGGCTTTTGGCTGTGG
CTATTGGGGCTGACTGTACTTATCCAGAGTTGACAGACAAACACAAGCTTGCAGATATATGTAAAAATCT
AAATTTCCGGCACAAAATGGCTCAATATGCCCAACGTGCATCAGTGGCTTTTCATACCCAGTTATTCTTC
AAAAGCAAAGGAATAGTAAGTGAAGAAGCCTATATTTTATTTGTAAGAAAGAATGCCATTGTGGTATTAA
TTCCAAAGTATGGTTTAGAAGGGACAGTCTTTTTTGAAGAAAAGGACAAACCAAACCCACAGCTTATTTA
TGATGATGAGATACCCTCACTTAAAATAGAAGATACAGTGTTCCATGTATTTGATAAAGTTAAAGTGAAA
ATCATGTTAGACTCATCTAATCTTCAACATCAGAAGATCCGAATGTCCCTGGTAGAACCACAGATACCAG
GAATAAGCATTCCTACTGATACTTCAAACATGGACCTTAATGGACCAAAGAAAAAGAAGATGAAGCTTGG
AAAATAG


Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites NotI-NotI     
ACCN NM_014953
Insert Size 3860 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014953.2, NP_055768.2
RefSeq Size 7320 bp
RefSeq ORF 2877 bp
Locus ID 22894
UniProt ID Q9Y2L1
Domains RNB, PINc
Protein Pathways RNA degradation
Gene Summary Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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