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NUDT15 (NM_018283) Human Untagged Clone
CAT＃: SC113594
NUDT15 (untagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15)
CNY 1,800.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MTH2; NUDT15D |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113594 sequence for NM_018283 edited (data generated by NextGen Sequencing)
ATGACGGCCAGCGCACAGCCGCGCGGGCGGCGGCCAGGAGTCGGAGTCGGAGTCGTGGTG ACCAGCTGCAAGCATCCGCGTTGCGTCCTCCTGGGGAAGAGGAAAGGCTCGGTTGGAGCT GGCAGTTTCCAACTCCCTGGAGGTCATCTGGAGTTCGGTGAAACCTGGGAAGAATGTGCT CAAAGGGAAACCTGGGAAGAAGCAGCTCTTCACCTGAAAAATGTTCACTTTGCCTCAGTT GTGAATTCTTTCATTGAGAAGGAGAATTACCATTATGTTACTATATTAATGAAAGGAGAA GTGGATGTGACTCATGATTCAGAACCAAAGAATGTAGAGCCTGAAAAAAATGAAAGTTGG GAGTGGGTTCCTTGGGAAGAACTACCTCCCCTGGACCAGCTTTTCTGGGGACTGCGTTGT TTAAAAGAACAAGGCTATGATCCATTTAAAGAAGATCTGAACCATCTGGTGGGATACAAA GGAAATCATCTCTAG Clone variation with respect to NM_018283.1 >OriGene 5' read for NM_018283 unedited
TGTCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCCGCGCGCT ATGACGGCCAGCGCACAGCCGCGCGGGCGGCGGCCAGGAGTCGGAGTCGGAGTCGTGGTG ACCAGCTGCAAGCATCCGCGTTGCGTCCTCCTGGGGAAGAGGAAAGGCTCGGTTGGAGCT GGCAGTTTCCAACTCCCTGGAGATCATCTGGAGTTCGGTGAAACCTGGGAAGAATGTGCT CAAAGGGAAACCTGGGAAGAAGCAGCTCTTCACCTGAAAAATGTTCACTTTGCCTCAGTT GTGAATTCTTTCATTGAGAAGGAGAATTACCATTATGTTACTATATTAATGAAAGGAGAA GTGGATGTGACTCATGATTCAGAACCAAAGAATGTAGAGCCTGAAAAAAATGAAAGTTGG GAGTGGGTTCCTTGGGAAGAACTACCTCCCCTGGACCAGCTTTTCTGGGGACTGCGTTGT TTAAAAGAACAAGGCTATGATCCATTTAAAGAAGATCTGAACCATCTGGTGGGATACAAA GGAAATCATCTCTAGGTGGCCAAGAAGATTTGATTTTCTTTAAAAAGACAAGAATAAGGT CTGGTTAGGGAATGAAAAATGTATACATTTCGGAACAACTCCATTTTATCTAAAAAAGTT CTTGTGATTGCCAGTTTATTTGCAGTCTCTTATGTATCCCCCCTCTTTCAGCCAGTACTT GAGAAAATTTTTCTGAATATGTCATTGAATTGTATTCCAGACACAGAATACATGATAAAT ACTGATATTATGGGTAATCTGCTTCCATATTTACTATGATATTACTGTGCAGNTTGNATT ACTAGCTGCATGGAGTAGATGCATCAAATTGCCTTAGTGTTTCTGTCAATAGAGACCCTG ATCAATTT >OriGene 3' read for NM_018283 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TAATTTCTTAAAATTTTTATTAATCATTTGCAAGTAACAAGAACCAAGTTTATATAAACA CATAAAAACATTTTTGGCAAATAGCTAATTTTATTTTTTTCACATATTAAAACCCACTAA AAATAACCATTTTTCTAACTAACCACAACAAAAATTAGGGAGAAAAGTACTGGTGCTTTA CATTTCTCCAAATCTCTTCAATGTCTGATGACAGACGAAAGCTGGAATCTTGGATCTGCT TTTGCATTCAGCCTATTACAATATCACACCAGATAGCCTCTTGAAAACTCCTCTGCATAC TCATGAAAAAATGAGGGAAAAAGATAAATGATGGCTGGGCGTTGGGGCTCATACCTGTAA TCCCAGCACTTTGGGAGGCTGAAATGGGCAAATCACTTGAGCTCAGGAGTTCCAGACCAG CCTGGCCAACATGGGGAAACCCCGTCTCCACTAAAAATACAAAAATTAGCCAGGTGTGGG GGTGGGTGCCTATAATCCCAGCTACTCGGGAAGCTGAGGTAGGAGAATTGCTTGAACCCA GGTGGCGGAGGCTGCAATGAGCTGAGATCGCGCCACTGCACTCCAATGTGGGCACCAGAG CAAGACTCCTTCTAAAATAAAAAAAGGGAAAGCTATAAATAGCGAAATGATGTCTTAGTG TTATAAAAACAGTTTTGACCCAAAAAACCCTTGAAGGCTTTTGAGGCCCCCCCAGACCAA ATTTTGACACCCACTGTTCTAGACGAATCTCTTCATATAAAGAAACCTTTCATAATAAGA ACCCCTGGCCTCAAAAAGAAGTAGTGGGAAACTCACCTCCAAGCCTTTAAAAGCCTGAAC AGAATATAAAGGGTTGCCCAACTCTTTTGGTTTAAATCCTACATGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_018283 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018283.1, NP_060753.1 |
| RefSeq Size | 2022 bp |
| RefSeq ORF | 495 bp |
| Locus ID | 55270 |
| UniProt ID | Q9NV35 |
| Domains | NUDIX |
| Gene Summary | This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210477 | NUDT15 (Myc-DDK-tagged)-Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15) |
CNY 1,800.00 |
|
| RC210477L1 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15), Myc-DDK-tagged |
CNY 4,200.00 |
|
| RC210477L2 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15), mGFP tagged |
CNY 5,890.00 |
|
| RC210477L3 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210477L4 | Lenti ORF clone of Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15), mGFP tagged |
CNY 5,890.00 |
|
| RG210477 | NUDT15 (tGFP-tagged) - Human nudix (nucleoside diphosphate linked moiety X)-type motif 15 (NUDT15) |
CNY 3,400.00 |
