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SPHK2 (NM_020126) Human Untagged Clone
CAT＃: SC113181
SPHK2 (untagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1
CNY 4,880.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SK-2; SK 2; SPK-2; SPK 2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC113181 sequence for NM_020126 edited (data generated by NextGen Sequencing)
ATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCAGGAGCTGACC GGGAGCTGGGGCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCATGGCCCCGCCC CCACCGCCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAGCC CGAGGCCCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACAGCGGCTGCGC CCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCAGGCTGC TGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCATCTACACCTAC CCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGCAGATGGGGCC GCCACCTACGAAGAGAACCGTGCCGAGGCCCAGCGCTGGGCCACTGCCCTCACCTGTCTG CTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCTACCTCGGCCG CCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAGTGGTGT AAGAACCACGTGCTTCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATCCAGACA GAACGACAGAACCACGCCCGGGAGCTGGTCCAGGGGCTGAGCCTGAGTGAGTGGGATGGC ATCGTCACGGTCTCGGGAGACGGGCTGCTCCATGAGGTGCTGAACGGGCTCCTAGATCGC CCTGACTGGGAGGAAGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCGGGCAAC GCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTCGACCTG TTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTGGACCTGCTCTCC GTGACGCTGGCCTCGGGCTCCCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGCTTCGTG TCAGATGTGGATATCCAGAGCGAGCGCTTCAGGGCCTTGGGCAGTGCCCGCTTCACACTG GGCACGGTGCTGGGCCTCGCCACACTGCACACCTACCGCGGACGCCTCTCCTACCTCCCC GCCACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGCCAAGTCGGAG CTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTGCATCGTTCTGTG TCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAACCC CTGCCCATCCTGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGACTGGGGTGGGGCT GGGGATGCTCCGCTGTCCCCGGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGGCA GCTCTACACTCACCCGTCTCCGAAGGGGCCCCCGTAATTCCCCCATCCTCTGGGCTCCCA CTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCGGCCCGCCCGACCACCTGCTG CCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGACGCTGGAGGGGGACTTTGTGCTC ATGTTGGCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCTCCGCATGCGCGC TTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGCTG CTGCGCCTTTTCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCGCAGCTG GGCTACGCCGCGGCCCGTGCCTTCCGCCTAGAGCCGCTCACACCACGCGGCGTGCTCACA GTGGACGGGGAGCAGGTGGAGTATGGGCCGCTACAGGCACAGATGCACCCTGGCATCGGT ACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGCCCTGA Clone variation with respect to NM_020126.4 >OriGene 5' read for NM_020126 unedited
TCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGGCACCAGCCGGAGACCCGG CGGGCCCAGTGTTGGAGAGCTGAAGGTCAGGCCAGGACAGTGAGACCTGACTCCTTGCTC CTACCAGCCTACTATGGCTTAAGACCCAGGGCCAGGGTCCCGTTGATGTAACAGAGCAGA GGACCAGCAGATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCA GGAGCTGACCGGGAGCTGGGGCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCAT GGCCCCGCCCCCACCGCCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTC CTACCCAGCCCGAGGCCCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACA GCGGCTGCGCCCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGT CTCAGGCTGCTGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCAT CTACACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGC AGATGGGGCCGCCACCTACGAAGAGAACCGTGCCGAAGCCCAGCGCTGGGCCACTGCCCT CACCTGTCTGCTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCT ACCTCGGCCGCCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTG GCAGTGGTGTAAGAAACACGTGCTTCCCATGATCTCTGAAGCTGGGCTGGTCTTCAAACT CATCCAGACAGAACGACAGAACCACGCCGGGAGCCTGGTCANGGGCTGAACCTGGATGAG TGGATGGCATCGTACGGTCTCGGGAAACGGCTGCTCATGAGTATTGA >OriGene 3' read for NM_020126 unedited
AGCTTGTACGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGAGAGGAATGAA GCGACTGCCTTTATTGCATAGACCTTTTCAATTGCTTTTTAGATTGGGGACGGAGGACGC GCATGAGACGAACAGGGGATATGAATTTCCCCGCCCCCACCCGCGGGGAGAGGAACATTA GTGCAAATCCTAGCGCCGGCCCCGGGGAACCTGCCCCTCCTGGGCTGATTGGCCAGCTAA ATGGAGGCACCGGAGTGGATGGGGCGAGGCTGCGGGCCCTGACCGGCCGACTCACTGAGG CCTACCCCAGCCAGTACATTCCAGGTCCTGTCATTGGGCGACGCGTAAAATAGACTCCGC CCTCAGCCATCCTGGCCGGGTAGGAGCAGGTGGCAAGCGTCAGGACTGAGCCCTGCCCCG TAAGGGCGGCCCCAGATCAGACGCCAGGCCCCGCCCTCATTGATCAGGCACTACCCTCGG GACGAGCCCATTTCTCTTTAACCGTGACGACGCCCACCTTCCAGCATCACGTCTGGTCCC ATGAAAGCGAGCGCAATCCTGAGACGGGGCCAGGGCCCCTGCCACCACAACTCTAGCAGC CAGGCCACACCCNCTAGCTCAGGCTCCGCCCCATTGGAATGTANGCCCCGCCCCCCGCGG GTACAAGCTTGGTTAGTTTCAAGGCTCCCGCCCCGGGCAGCCAGNAGGCCCAGTGAGCAG TGTACCGATGCCAGGGTGCATCTGTGCCTGTAGCGGCCATACTCCAACTGCTCCCCGTCC ACTGTGAGCAGCCGCCGTGTGTGAGCGGCTCTAGGCGGAGGGCCAGGGCCGGGGCGTATC CCACCTGGCGACAGCCCAGGCTGGAGTGGGCTACCACGCTCCATGGCCAAAAAAAGGCGC AGCAACGCAGCCCCGCGAGATGCCGCTACGCACCCAGCCAGGTGCACCAAGCCGTCGTCG AACG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020126 |
| Insert Size | 2900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020126.3, NP_064511.2 |
| RefSeq Size | 3012 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 56848 |
| UniProt ID | Q9NRA0 |
| Domains | DAGKc |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway |
| Gene Summary | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a). |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Inhibiting sphingosine kinase 2 mitigates mutant Huntingtin-induced neurodegeneration in neuron models of Huntington disease
,Moruno-Manchon, JF;Uzor, NE;Blasco-Conesa, MP;Mannuru, S;Putluri, N;Furr-Stimming, EE;Tsvetkov, AS;,
Hum. Mol. Genet.
,PubMed ID 28175299
[SPHK2]
|
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Intracellular sphingosine kinase 2-derived sphingosine-1-phosphate mediates epidermal growth factor-induced ezrin-radixin-moesin phosphorylation and cancer cell invasion
,Adada, MM;Canals, D;Jeong, N;Kelkar, AD;Hernandez-Corbacho, M;Pulkoski-Gross, MJ;Donaldson, JC;Hannun, YA;Obeid, LM;,
FASEB J.
,PubMed ID 26209696
[SPHK2]
|
|
Sphingosine Kinase as a Regulator of Calcium Entry through Autocrine Sphingosine 1-Phosphate Signaling in Thyroid FRTL-5 Cells
,Dan Gratschev, Christoffer Löf, Jari Heikkilä, Anders Björkbom, Pramod Sukumaran, Ari Hinkkanen, J. Peter Slotte, and Kid Törnquist,
Endocrinology, Oct 2009; 10.1210/en.2009-0288
[SPHK2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203019 | SPHK2 (Myc-DDK-tagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1 |
CNY 7,304.00 |
|
| RC203019L1 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC203019L2 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC203019L3 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC203019L4 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, mGFP tagged |
CNY 9,704.00 |
|
| RG203019 | SPHK2 (tGFP-tagged) - Human sphingosine kinase 2 (SPHK2), transcript variant 1 |
CNY 8,904.00 |
|
| SC320762 | SPHK2 (untagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1 |
CNY 4,880.00 |
