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SPHK2 (NM_020126) Human Tagged ORF Clone
CAT＃: RC203019
- TrueORF®
SPHK2 (Myc-DDK-tagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1
ORF Plasmid: tGFP
"NM_020126" in other vectors (7)
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CNY 7,304.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SK-2; SK 2; SPK-2; SPK 2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203019 representing NM_020126
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCAGGAGCTGACCGGGAGCTGGG GCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCATGGCCCCGCCCCCACCGCCACTGGCTGCCAG CACCCCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAGCCCGAGGCCCACGCTTTGCCCTCACCCTTACA TCGCAGGCCCTGCACATACAGCGGCTGCGCCCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGT TGGCCGAGGTCTCAGGCTGCTGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCAT CTACACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGCAGATGGGGCC GCCACCTACGAAGAGAACCGTGCCGAGGCCCAGCGCTGGGCCACTGCCCTCACCTGTCTGCTCCGAGGAC TGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCTACCTCGGCCGCCCCGGTTGCTTCTATTGGT CAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAGTGGTGTAAGAACCACGTGCTTCCCATGATCTCTGAA GCTGGGCTGTCCTTCAACCTCATCCAGACAGAACGACAGAACCACGCCCGGGAGCTGGTCCAGGGGCTGA GCCTGAGTGAGTGGGATGGCATCGTCACGGTCTCGGGAGACGGGCTGCTCCATGAGGTGCTGAACGGGCT CCTAGATCGCCCTGACTGGGAGGAAGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCGGGCAAC GCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTCGACCTGTTGCTCAACT GCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTGGACCTGCTCTCCGTGACGCTGGCCTCGGGCTC CCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGCTTCGTGTCAGATGTGGATATCCAGAGCGAGCGCTTC AGGGCCTTGGGCAGTGCCCGCTTCACACTGGGCACGGTGCTGGGCCTCGCCACACTGCACACCTACCGCG GACGCCTCTCCTACCTCCCCGCCACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGC CAAGTCGGAGCTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTGCATCGTTCTGTG TCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAACCCCTGCCCATCC TGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGACTGGGGTGGGGCTGGGGATGCTCCGCTGTCCCC GGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGGCAGCTCTACACTCACCCGTCTCCGAAGGGGCC CCCGTAATTCCCCCATCCTCTGGGCTCCCACTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCG GCCCGCCCGACCACCTGCTGCCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGACGCTGGAGGGGGA CTTTGTGCTCATGTTGGCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCTCCGCATGCGCGC TTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGCTGCTGCGCCTTT TCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCGCAGCTGGGCTACGCCGCGGCCCGTGC CTTCCGCCTAGAGCCGCTCACACCACGCGGCGTGCTCACAGTGGACGGGGAGCAGGTGGAGTATGGGCCG CTACAGGCACAGATGCACCCTGGCATCGGTACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGC CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203019 representing NM_020126
Red=Cloning site Green=Tags(s) MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTPLLHGEFGSYPARGPRFALTLT SQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGA ATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISE AGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGN ALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERF RALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSV SDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGA PVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHAR FDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGP LQAQMHPGIGTLLTGPPGCPGREP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020126 |
| ORF Size | 1962 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020126.5 |
| RefSeq Size | 3012 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 56848 |
| UniProt ID | Q9NRA0 |
| Domains | DAGKc |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway |
| MW | 69 kDa |
| Gene Summary | This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203019L1 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC203019L2 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC203019L3 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC203019L4 | Lenti ORF clone of Human sphingosine kinase 2 (SPHK2), transcript variant 1, mGFP tagged |
CNY 9,704.00 |
|
| RG203019 | SPHK2 (tGFP-tagged) - Human sphingosine kinase 2 (SPHK2), transcript variant 1 |
CNY 8,904.00 |
|
| SC113181 | SPHK2 (untagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1 |
CNY 2,950.00 |
|
| SC320762 | SPHK2 (untagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1 |
CNY 2,950.00 |
