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NCLN (NM_020170) Human Untagged Clone
CN¥ 6,296.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NET59 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111361 sequence for NM_020170 edited (data generated by NextGen Sequencing)
ATGCTGGAGGAAGCGGGCGAGGTGCTGGAGAACATGCTGAAGGCGTCTTGTCTGCCGTTC GGCTTCATCGTCTTCCTGCCCGCTGTGCTGCTGCTGGTGGCGCCGCCGCTGCCTGCCGCC GACGCCGCGCACGAGTTCACCGTGTACCGCATGCAGCAGTACGACCTGCAGGGCCAGCCC TACGGCACACGGAATGCAGTGCTGAACACGGAGGCGCGCACGATGGCGGCGGAGGTGCTG AGCCGCCGCTGCGTGCTCATGCGGCTACTGGACTTCTCCTACGAGCAGTACCAGAAGGCC CTGCGGCAGTCGGCGGGCGCCGTGGTCATCATCCTGCCCAGGGCCATGGCCGCCGTGCCC CAGGACGTCGTCCGGCAATTCATGGAGATCGAGCCGGAGATGCTGGCCATGGAGACCGCC GTCCCCGTGTACTTTGCCGTGGAGGACGAGGCCCTGCTGTCTATCTACAAGCAGACCCAG GCTGCCTCCGCCTCCCAGGGCTCCGCCTCTGCTGCTGAAGTACTGCTGCGCACGGCCACT GCCAACGGCTTCCAGATGGTCACCAGCGGGGTACAGAGCAAGGCCGTGAGTGACTGGCTG ATTGCCAGCGTGGAGGGACGGCTGACGGGGCTGGGCGGAGAGGACCTTCCCACCATCGTC ATCGTGGCCCACTACGACGCCTTTGGAGTGGCCCCCTGGCTGTCGCTGGGCGCGGACTCC AACGGGAGCGGCGTCTCTGTGCTGCTGGAGCTGGCACGCCTCTTCTCCCGGCTCTACACC TACAAGCGCACGCACGCCGCCTACAACCTCCTGTTCTTTGCGTCTGGAGGAGGCAAGTTT AACTACCAGGGAACCAAGCGCTGGCTGGAAGACAACCTGGACCACACAGACTCCAGCCTG CTTCAGGACAATGTGGCCTTCGTGCTGTGCCTGGACACCGTGGGCCGGGGCAGCAGCCTG CACCTGCACGTGTCCAAGCCGCCTCGGGAGGGCACCCTGCAGCACGCCTTCCTGCGGGAG CTGGAGACGGTGGCCGCGCACCAGTTCCCTGAGGTACGGTTCTCCATGGTGCACAAGCGG ATCAACCTGGCGGAGGACGTGCTGGCCTGGGAGCACGAGCGCTTCGCCATCCGCCGACTG CCCGCCTTCACGCTGTCCCACCTGGAGAGCCACCGTGACGGCCAGCGCAGCAGCATCATG GACGTGCGGTCCCGGGTGGATTCTAAGACCCTGACCCGTAACACGAGGATCATTGCAGAG GCCCTGACTCGAGTCATCTACAACCTGACAGAGAAGGGGACACCCCCAGACATGCCGGTG TTCACAGAGCAGATGCAGATCCAGCAGGAGCAGCTGGACTCGGTGATGGACTGGCTCACC AACCAGCCGCGGGCCGCGCAGCTGGTGGACAAGGACAGCACCTTCCTCAGCACGCTGGAG CACCACCTGAGCCGCTACCTGAAGGACGTGAAGCAGCACCACGTCAAGGCTGACAAGCGG GACCCAGAGTTTGTCTTCTACGACCAGCTGAAGCAAGTGATGAATGCGTACAGAGTCAAG CCGGCCGTCTTTGACCTGCTCCTGGCCGTTGGCATTGCTGCCTACCTCGGCATGGCCTAC GTGGCTGTCCAGCACTTCAGCCTCCTCTACAGGACCGTCCAGAGGCTGCTCGTGAAGGCC AAGACACAGTGA Clone variation with respect to NM_020170.3 58 c=>t;618 g=>a;1587 t=>c;1652 a=>g >OriGene 5' read for NM_020170 unedited
TTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG AATTCGGCACGAGGGGTGAGTCCGCGGGAGCCGCCGCCGCCGCCGTCCCGTCCCAGCTGC CGCCCCGCGCGGCCCCGCCGCCGGCCAGGATGCTGGAGGAAGCGGGCGAGGTGCTGGAGA ACATGCTGAAGGCGTCTTGTCTGCCGTTCGGCTTCATCGTCTTCCTGCCCGCTGTGCTGC TGCTGGTGGCGCCGCCGCTGCCTGCCGCCGACGCCGCGCACGAGTTCACCGTGTACCGCA TGCAGCAGTACGACCTGCAGGGCCAGCCCTACGGCACACGGAATGCAGTGCTGAACACGG AGGCGCGCACGATGGCGGCGGAGGTGCTGAGCCGCCGCTGCGTGCTCATGCGGCTACTGG ACTTCTCCTACGAGCAGTACCAGAAGGCCCTGCGGCAGTCGGCGGGCGCCGTGGTCATCA TCCTGCCCAGGGCCATGGCCGCCGTGCCCCAGGACGTCGTCCGGCAATTCATGGAGATCG AGCCGGAGATGCTGGCCATGGAGACCGCCGTCCCCGTGTACTTTGCCGTGGAGGACGAGG CCCTGCTGTCTATCTACAAGCAGACCCAGGCTGCCTCCGCCTCCCAGGGCTCCGCCTCTG CTGCTGAAGTACTGCTGCGCACGGCCACTGCCAACGGCTTCCAGATGGTCACCAGCGGNG TACAGAGCAAGGCCGTGAGTGACTGGCTGATTGCCAGCGTGGAGGNACGGCTGACGGAGC TGGNCNGANAGGACCTTCCACATCGTCATCGTGGCCACTACGACGCTTTGGNAGTGGCCC CCTGGCTGTCNCTGGNCGCAGACTCAACGGNNAGCGCGTCTCTGTGCTGCTGGAGCTGGC ACGCCTCTT >OriGene 3' read for NM_020170 unedited
ATACTTATGTACGTGGCCGCTTTTTANGATCGAGTTTTTTTTTTTTTTTTTTGGCGATCA TTCAATATGGCTTTATTTTCTCATCGGCCGGCTGCATCCCACACCAGCCTGAGCCCCAGA CGGTCAGTCAGTGCATCCATCCTGACGACCTGTCCAGGAAGGAAGGAAGGAAGCTCCCTC CAAAATGCTGGCCCCGTGGCACCCAGACAGGAGCCTGCTGGCTAGCATGCAAGCACTCCC CAAATTCTCTCATGGTGACAAGGTGGCAGCTGCCTGGGTCCCAGAGTCTCAGCACAAGTG CCCAGAGGCCAGTGGTGATGGGCCCTTGGGCAAGTCTCTGGAGGAGGCGGGCAACCCACA GGGCAGACGGGAGAAGAGGGTCCCCTGCCAACCTGGAGGAAAGAGGGGTGGTTGTCCCCT CACCCCTCCAGGCTGCAGGTTCTGGAGGGCAGAGGTGCTGGCCGAAGAACTGGTGGCCAA AGTGAGACCCCCTGGTCCTATCTGGACAGAACCCCAGGCTCCCATGGGCCGGCCCAGGTC CTTCGGACAGCAAACACCTCCCTGGGCCACGGAAGGGAGACGGCTGCGGGTGCCAGGCCA GGACTTGCCCAGCTCAAGTCCCTTCCCCACCAAGCCTGCTCGGCACGCACTGGGTAGGTT ATATTGCTAAAGAACGGACACACCGGGGGTCTCCCTGAGCTCAACACGGGCCTCCTTCTC TCCACACACACACGACGCCTGGTCTGCGGGGTGGGNAGGTTCTGTCCAAGGCTTCCCGAG GGCCCGGACCTTCCTTCTGAGTTCCAGAAAGGTGGCCCCANAGCCATGCCCAAGTTCTCT CCTTCCAGAAACGTTCTACCTGACGCCCCACCCCGAACATCCTTGCATTGAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020170 |
| Insert Size | 4100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020170.3, NP_064555.2 |
| RefSeq Size | 3749 bp |
| RefSeq ORF | 1692 bp |
| Locus ID | 56926 |
| UniProt ID | Q969V3 |
| Protein Families | Transmembrane |
| Gene Summary | May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.[UniProtKB/Swiss-Prot Function] |
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Resources
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