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HSD3B7 (NM_025193) Human Untagged Clone
CAT＃: SC110313
HSD3B7 (untagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CBAS1; PFIC4; SDR11E3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC110313 sequence for NM_025193 edited (data generated by NextGen Sequencing)
ATGGCCGACTCTGCACAGGCCCAGAAGCTGGTGTACCTGGTCACAGGGGGCTGTGGCTTC CTGGGAGAGCACGTGGTGCGAATGCTGCTGCAGCGGGAGCCCCGGCTCGGGGAGCTGCGG GTCTTTGACCAACACCTGGGTCCCTGGCTGGAGGAGCTGAAGACAGGGCCTGTGAGGGTG ACTGCCATCCAGGGGGACGTGACCCAGGCCCATGAGGTGGCAGCAGCTGTGGCCGGAGCC CATGTGGTCATCCACACGGCTGGGCTGGTAGACGTGTTTGGCAGGGCCAGTCCCAAGACC ATCCATGAGGTCAACGTGCAGGGTACCCGGAACGTGATCGAGGCTTGTGTGCAGACCGGA ACACGGTTCCTGGTCTACACCAGCAGCATGGAAGTTGTGGGGCCTAACACCAAAGGTCAC CCCTTCTACAGGGGCAACGAAGACACCCCATACGAAGCAGTGCACAGGCACCCCTATCCT TGCAGCAAGGCCCTGGCCGAGTGGCTGGTCCTGGAGGCCAACGGGAGGAAGGTCCGTGGG GGGCTGCCCCTGGTGACGTGTGCCCTTCGTCCCACGGGCATCTACGGTGAAGGCCACCAG ATCATGAGGGACTTCTACCGCCAGGGCCTGCGCCTGGGAGGTTGGCTCTTCCGGGCCATC CCGGCCTCTGTGGAGCATGGCCGGGTCTATGTGGGCAATGTTGCCTGGATGCACGTGCTG GCAGCCCGGGAGCTGGAGCAGCGGGCAGCCCTGATGGGCGGCCAGGTATACTTCTGCTAC GATGGATCACCCTACAGGAGCTACGAGGATTTCAACATGGAGTTCCTGGGCCCCTGCGGA CTGCGGCTGGTGGGCGCCCGCCCATTGCTGCCCTACTGGCTGCTGGTGTTCCTGGCTGCC CTCAATGCCCTGCTGCAGTGGCTGCTGCGGCCACTGGTGCTCTACGCACCCCTGCTGAAC CCCTACACGCTGGCCGTGGCCAACACCACCTTCACCGTCAGCACCGACAAGGCTCAGCGC CATTTCGGCTATGAGCCCCTGTTCTCGTGGGAGGATAGCCGGACCCGCACCATTCTCTGG GTACAGGCCGCTACGGGTTCAGCCCAGTGA Clone variation with respect to NM_025193.3 748 a=>g;1068 t=>c >OriGene 5' read for NM_025193 unedited
ATTATGTATACGACTCACTATAGGGCGGCNCGCGAATCGGCACCAGGAGGCCCCTCCAGG CCAGTCTGGGCACCCTGGGATAGCGGCTGCAGCCATCAGCAGGGGCAGACGGCAGGTGGC CTGGTTGCTGCAGCTCCCAGGATCAGCTCTGCCCTCCCGCCAAACGCCAGCCTCGTCACC GCTCCAGGGCACCTCCAGCAGTAACAGGTGGTTGCAGCAGGTGGCAGCCAGCCCCTGGAT GAGCCAAGGTCTCTTCCCCAGCCAGGCATGGCCGACTCTGCACAGGCCCAGAAGCTGGTG TACCTGGTCACAGGGGGCTGTGGCTTCCTGGGAGAGCACGTGGTGCGAATGCTGCTGCAG CGGGAGCCCCGGCTCGGGGAGCTGCGGGTCTTTGACCAACACCTGGGTCCCTGGCTGGAG GAGCTGAAGACAGGGCCTGTGAGGGTGACTGCCATCCAGGGGGACGTGACCCAGGCCCAT GAGGTGGCAGCAGCTGTGGCCGGAGCCCATGTGGTCATCCACACGGCTGGGCTGGTAGAC GTGTTTGGCAGGGCCAGTCCCAAGACCATCCATGAGGTCAACGTGCAGGGTACCCGGAAC GTGATCGAGGCTTGTGTGCAGACCGGAACACGGTTCCTGGTCTACACCAGCAGCATGGAA GTTGTGGGGCCTAACACCAAAGGTCACCCCTTCTACAGGGGCAACGAAGACACCCCATAC GAAGCAGTGCACAGGCACCCCTATCCTTGCAGCAAGGCCCTGGCCGAGTGGCTGGTCCTG GAGGCCACGGGAGGAAGGTCCGTGGNGGGCTGCCCCTGGTGACGTGTGCCTTTCGTCCCA CGGCATCTACGGTGAAGGCCACCAGATCATGAGGNACTNCTACCGCCAGGNCCTGCGCCT GGGAGGNTGGCTCTTCGGNCCATCCNGNCTCTGTGGACATGGCCGGTCTAGTGGGCATGT GCCTGGAGCACTGCTGCACCCGGAACTG >OriGene 3' read for NM_025193 unedited
NGGGGGNNNNNNNNNNNNNTTTTNNNNNNANNNNTAATCTNTGNNACGCGGNCCGCATTT NANGATCGAGNNNTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTGGGGATGAAGGT TTTAAACCGGGGTCAACCAGGTACAACATTGGGGACATCCCCCAGCCCAAGGCTGGCGAT GTGCTGGGAAGGAACTTAAGTAAAAAGGGGGGAAGTGAAAACATAAGGAGGCCCTAACCC TCCAAAGGGGAATTTTAAAACAAACAGTTGAAGGGGAGGCCTTTGAAAAACAATGGGAAC ATCACCTCCCAAAAAGGGACTGAGGGGGCTGGAGGAACCAAAACCGCCTTTGCACTTTGC ACCGAGGGTCCCGTGTGGCTGTCAAGAAAACAACGTAAGCCTGTGGAGCCTCTGGTCGCT TTCTGCTCAAAAAGCCAAAAGAAAACTGGGGCAGGCCACCAGGGCACCAAACACTTACCA AGCGGGCATTANGACTGGGATGAGAGGACAGAGGAAGGACACTCTGTCCCCAAACCCTTC CGTGTCCTGACGGGCCTAGAACTTGGAGGTGTCAAGACACAACACAGACTCAAGGCTTCT GGATTGAAAATGTGGGAGGCTTGGGCCAAGCGCGGTGGCTCACACTTGTAGTTCCAACGT TTTGGGAAGGCCACGTGGGAAGATCACTTGAGCCCAGAGTTCAAGACCAAGCTGAGCATA TAGTGAGACCCTGTCCTTAAAACAACAAAAAAAAGCCAGTGTGTTGGCGTGCACCTGTGG CCCNACTGTTCACGAAGCTTAGGCAGGAGATATTCTTAAACCACAGAGGTGTAGGGGGCA TGACCTATTATTACCCCACTTGCCTCCAGTTTGGGGACCAAGCAGAACCTGTCTCAAAAG AAAAAGAAATTGAAGGGCTTTANAAAGGAAGNCATTCTGGCCCAGAGCCTGGCTCTGCTC TGAATGCACTGCCCTTCCGCACAGGGTGAGGGCTCGGCCTCGGGGTATGGCTGATCTGGG CTCCAGGCAACCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_025193 |
| Insert Size | 2210 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025193.2, NP_079469.2 |
| RefSeq Size | 2189 bp |
| RefSeq ORF | 1110 bp |
| Locus ID | 80270 |
| UniProt ID | Q9H2F3 |
| Domains | 3Beta_HSD |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Primary bile acid biosynthesis |
| Gene Summary | This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] Transcript Variant: This variant (1) encodes the longer isoform (a). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202468 | HSD3B7 (Myc-DDK-tagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1 |
CNY 3,656.00 |
|
| RC202468L1 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202468L2 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC202468L3 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202468L4 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG202468 | HSD3B7 (tGFP-tagged) - Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1 |
CNY 5,256.00 |
