HSD3B7 (NM_025193) Human Tagged ORF Clone
CAT#: RC202468
HSD3B7 (Myc-DDK-tagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1
ORF Plasmid: tGFP
"NM_025193" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CBAS1; PFIC4; SDR11E3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202468 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACTCTGCACAGGCCCAGAAGCTGGTGTACCTGGTCACAGGGGGCTGTGGCTTCCTGGGAGAGC ACGTGGTGCGAATGCTGCTGCAGCGGGAGCCCCGGCTCGGGGAGCTGCGGGTCTTTGACCAACACCTGGG TCCCTGGCTGGAGGAGCTGAAGACAGGGCCTGTGAGGGTGACTGCCATCCAGGGGGACGTGACCCAGGCC CATGAGGTGGCAGCAGCTGTGGCCGGAGCCCATGTGGTCATCCACACGGCTGGGCTGGTAGACGTGTTTG GCAGGGCCAGTCCCAAGACCATCCATGAGGTCAACGTGCAGGGTACCCGGAACGTGATCGAGGCTTGTGT GCAGACCGGAACACGGTTCCTGGTCTACACCAGCAGCATGGAAGTTGTGGGGCCTAACACCAAAGGTCAC CCCTTCTACAGGGGCAACGAAGACACCCCATACGAAGCAGTGCACAGGCACCCCTATCCTTGCAGCAAGG CCCTGGCCGAGTGGCTGGTCCTGGAGGCCAACGGGAGGAAGGTCCGTGGGGGGCTGCCCCTGGTGACGTG TGCCCTTCGTCCCACGGGCATCTACGGTGAAGGCCACCAGATCATGAGGGACTTCTACCGCCAGGGCCTG CGCCTGGGAGGTTGGCTCTTCCGGGCCATCCCGGCCTCTGTGGAGCATGGCCGGGTCTATGTGGGCAATG TTGCCTGGATGCACGTGCTGGCAGCCCGGGAGCTGGAGCAGCGGGCAACCCTGATGGGCGGCCAGGTATA CTTCTGCTACGATGGATCACCCTACAGGAGCTATGAGGATTTCAACATGGAGTTCCTGGGCCCCTGCGGA CTGCGGCTGGTGGGCGCCCGCCCATTGCTGCCCTACTGGCTGCTGGTGTTCCTGGCTGCCCTCAATGCCC TGCTGCAGTGGCTGCTGCGGCCACTGGTGCTCTACGCACCCCTGCTGAACCCCTACACGCTGGCCGTGGC CAACACCACCTTCACCGTCAGCACCGACAAGGCTCAGCGCCATTTCGGCTATGAGCCCCTGTTCTCGTGG GAGGATAGCCGGACCCGTACCATTCTCTGGGTACAGGCCGCTACGGGTTCAGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202468 protein sequence
Red=Cloning site Green=Tags(s) MADSAQAQKLVYLVTGGCGFLGEHVVRMLLQREPRLGELRVFDQHLGPWLEELKTGPVRVTAIQGDVTQA HEVAAAVAGAHVVIHTAGLVDVFGRASPKTIHEVNVQGTRNVIEACVQTGTRFLVYTSSMEVVGPNTKGH PFYRGNEDTPYEAVHRHPYPCSKALAEWLVLEANGRKVRGGLPLVTCALRPTGIYGEGHQIMRDFYRQGL RLGGWLFRAIPASVEHGRVYVGNVAWMHVLAARELEQRATLMGGQVYFCYDGSPYRSYEDFNMEFLGPCG LRLVGARPLLPYWLLVFLAALNALLQWLLRPLVLYAPLLNPYTLAVANTTFTVSTDKAQRHFGYEPLFSW EDSRTRTILWVQAATGSAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_025193 |
ORF Size | 1107 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025193.2, NP_079469.2 |
RefSeq Size | 2203 bp |
RefSeq ORF | 1110 bp |
Locus ID | 80270 |
UniProt ID | Q9H2F3 |
Domains | 3Beta_HSD |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Primary bile acid biosynthesis |
MW | 41 kDa |
Gene Summary | This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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3β-Isoobeticholic acid efficiently activates the farnesoid X receptor (FXR) due to its epimerization to 3α-epimer by hepatic metabolism
,Stefela, A;Kaspar, M;Drastik, M;Holas, O;Hroch, M;Smutny, T;Skoda, J;Hutníková, M;Pandey, AV;Micuda, S;Kudova, E;Pavek, P;,
J. Steroid Biochem. Mol. Biol.
,PubMed ID 32505574
[HSD3B7]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202468L1 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202468L2 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC202468L3 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC202468L4 | Lenti ORF clone of Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG202468 | HSD3B7 (tGFP-tagged) - Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1 |
CNY 5,256.00 |
|
SC110313 | HSD3B7 (untagged)-Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1 |
CNY 3,656.00 |