United States
Germany
Japan
United Kingdom
China
MLKL (NM_152649) Human Untagged Clone
CAT＃: SC108101
MLKL (untagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1
CNY 5,488.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hMLKL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_152649 edited
ATGGAAAATTTGAAGCATATTATCACCCTTGGCCAGGTCATCCACAAACGGTGTGAAGAG ATGAAATACTGCAAGAAACAGTGCCGGCGCCTGGGCCACCGCGTCCTCGGCCTGATCAAG CCTCTGGAGATGCTCCAGGACCAAGGAAAGAGGAGCGTGCCCTCTGAGAAGTTAACCACA GCCATGAACCGCTTCAAGGCTGCCCTGGAGGAGGCTAATGGGGAGATAGAAAAGTTCAGC AATAGATCCAATATCTGCAGGTTTCTAACAGCAAGCCAGGACAAAATACTCTTCAAGGAC GTGAACAGGAAGCTGAGTGATGTCTGGAAGGAGCTCTCGCTGTTACTTCAGGTTGAGCAA CGCATGCCTGTTTCACCCATAAGCCAAGGAGCGTCCTGGGCACAGGAAGATCAGCAGGAT GCAGACGAAGACAGGCGAGCTTTCCAGATGCTAAGAAGAGATAATGAAAAAATAGAAGCT TCACTGAGACGATTAGAAATCAACATGAAAGAAATCAAGGAAACTTTGAGGCAGTATTTA CCACCAAAATGCATGCAGGAGATCCCGCAAGAGCAAATCAAGGAGATCAAGAAGGAGCAG CTTTCAGGATCCCCGTGGATTCTGCTAAGGGAAAATGAAGTCAGCACACTTTATAAAGGA GAATACCACAGAGCTCCAGTGGCCATAAAAGTATTCAAAAAACTCCAGGCTGGCAGCATT GCAATAGTGAGGCAGACTTTCAATAAGGAGATCAAAACCATGAAGAAATTCGAATCTCCC AACATCCTGCGTATATTTGGGATTTGCATTGATGAAACAGTGACTCCGCCTCAATTCTCC ATTGTCATGGAGTACTGTGAACTCGGGACCCTGAGGGAGCTGTTGGATAGGGAAAAAGAC CTCACACTTGGCAAGCGCATGGTCCTAGTCCTGGGGGCAGCCCGAGGCCTATACCGGCTA CACCATTCAGAAGCACCTGAACTCCACGGAAAAATCAGAAGCTCAAACTTCCTGGTAACT CAAGGCTACCAAGTGAAGCTTGCAGGATTTGAGTTGAGGAAAACACAGACTTCCATGAGT TTGGGAACTACGAGAGAAAAGACAGACAGAGTCAAATCTACAGCATATCTCTCACCTCAG GAACTGGAAGATGTATTTTATCAATATGATGTAAAGTCTGAAATATACAGCTTTGGAATC GTCCTCTGGGAAATCGCCACTGGAGATATCCCGTTTCAAGGCTGTAATTCTGAGAAGATC CGCAAGCTGGTGGCTGTGAAGCGGCAGCAGGAGCCACTGGGTGAAGACTGCCCTTCAGAG CTGCGGGAGATCATTGATGAGTGCCGGGCCCATGATCCCTCTGTGCGGCCCTCTGTGGAT GAAATCTTAAAGAAACTCTCCACCTTTTCTAAGTAG >OriGene 5' read for NM_152649 unedited
CGGCCAAAAACTCGGCACGAGCCGGAGGGCACTGGTCAGAAACAGCCATCCAATGGCTGA GTTGAGGGACCCTGCTCAAGTGCAGCTGCAGTGGCCGGGGTTTCCCTCAGGAGGCGGTCG TGGGAAAGAAGGTGGAAGAGCGAGCTTTTTGGAACTGTGCACGGGACAGATTGGACGCAC ACCCTTCGTGAGGCGCGAAGGCATGGAAAATTTGAAGCATATTATCACCCTTGGCCAGGT CATCCACAAACGGTGTGAAGAGATGAAATACTGCAAGAAACAGTGCCGGCGCCTGGGCCA CCGCGTCCTCGGCCTGATCAAGCCTCTGGAGATGCTCCAGGACCAAGGAAAGAGGAGCGT GCCCTCTGAGAAGTTAACCACAGCCATGAACCGCTTCAAGGCTGCCCTGGAGGAGGCTAA TGGGGAGATAGAAAAGTTCAGCAATAGATCCAATATCTGCAGGTTTCTAACAGCAAGCCA GGACAAAATACTCTTCAAGGACGTGAACAGGAAGCTGAGTGATGTCTGGAAGGAGCTCTC GCTGTTACTTCAGGTTGAGCAACGCATGCCTGTTTCACCCATAAGCCAAGGAGCGTCCTG NGCACAGGAAGATCAGCAGGATGCAGACGAAGACAGGCGAGCTTTCCAGATGCTAAGAAG AGATAATGAAAAAATAGAAGCTTCACTGAGACGATTAGAAATCAACATGAAAGAAATCAA GGAAACTTTGAGGCAGTATTTACCACCAAAATGCATGCANGAGATCCCGNCAGAGCANAT CAAGGAGATCAAGAAGGAGCAGCTTTCAGGATCCCCGTGGATTCTGCTAAGGGGAAATGA AGTCAGCACACTTTATAAAGGAGAATACCACAGAGCTTCAGTGGCCATAAAGTATTCAAA AACTCC >OriGene 3' read for NM_152649 unedited
GGCGCAATTTTCGGGTTGTTGATTCATCTCTGGATGTGTAGATGGGCAATTTGGGCTGGG ATCTGCTTTAGTGGTTCTTCTGGTCTTGATTGGCCTCATTCATGTGTTACCTGCCATGTT GGTTGGGCTACTATGACTTGCCCTAGGAAAGCATGTTTCTGCTTCATGTGGTCTCTCATT CTCCAGCATGCTCGCTCAACATGTTCATGGCTGGTTTTTGTTTTAACACACATTTATTAT GCCTCAGGAAACCCACGCTTGGGACGTGGGCTACAGTCACTTCATCCACATACCATTGAT ACAAATCAAGTCACAGCGCCTGCCCGGCATTCTAATGGTTGGTGAAATAGACTTCATTTA TACAAGTCCGTAACTGCCTATTCCATTGTTAAGGAGTGTGTATGCACCGGATGAAGCATT GGAGAGAGCATCTCGCACACTTAGTGTGCAAAGTCTGGTGTGGCGATACAACTTGGGCCT GGGATGTGAAGTGATAAATAAAAAGATAATATCATCAAAAAAGAAACCCAGGACACCGCA CGATCGATAGAACAATACGGGGCGCAGAGCTGAGGGAACCACCCAACCAAATCCCAACAC TACTGCATGAGCTCGGCGAGCCGACCCGCGCCGCCTGGCCGAGCCGGCCGGGCGCCGGGG GAGGCAGGAATCGGACGATGCGCCAGCGACCAAACAGCAAGACCTCCCCCTACACACCCC AGCGGGGCACGCGGGGCAGAGACTTCATCTGGCCACGTCCACACTTCACCCACCAGCGAA ACGCCACATCGCGAACCCCCGCCCGCGCGACGGGGAAAGGGGAGGGAAAGGCGGGGGGAC GGGCGTTCAGGGCGGACCGATGCGCCACCACGGGCGACGACCGGCCGCCCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_152649 |
| Insert Size | 2500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_152649.1, NP_689862.1 |
| RefSeq Size | 2496 bp |
| RefSeq ORF | 1416 bp |
| Locus ID | 197259 |
| UniProt ID | Q8NB16 |
| Domains | pkinase |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1, also known as MLKLshRNA-A) represents the longer transcript and encodes the longer isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213152 | MLKL (Myc-DDK-tagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1 |
CNY 5,488.00 |
|
| RC213152L1 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC213152L2 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC213152L3 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC213152L4 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG213152 | MLKL (tGFP-tagged) - Human mixed lineage kinase domain-like (MLKL), transcript variant 1 |
CNY 7,088.00 |
