MLKL (NM_152649) Human Tagged ORF Clone
CAT#: RC213152
- TrueORF®
MLKL (Myc-DDK-tagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1
ORF Plasmid: tGFP
"NM_152649" in other vectors (6)
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CNY 5,488.00
Cited in 6 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | hMLKL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213152 representing NM_152649
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAATTTGAAGCATATTATCACCCTTGGCCAGGTCATCCACAAACGGTGTGAAGAGATGAAATACT GCAAGAAACAGTGCCGGCGCCTGGGCCACCGCGTCCTCGGCCTGATCAAGCCTCTGGAGATGCTCCAGGA CCAAGGAAAGAGGAGCGTGCCCTCTGAGAAGTTAACCACAGCCATGAACCGCTTCAAGGCTGCCCTGGAG GAGGCTAATGGGGAGATAGAAAAGTTCAGCAATAGATCCAATATCTGCAGGTTTCTAACAGCAAGCCAGG ACAAAATACTCTTCAAGGACGTGAACAGGAAGCTGAGTGATGTCTGGAAGGAGCTCTCGCTGTTACTTCA GGTTGAGCAACGCATGCCTGTTTCACCCATAAGCCAAGGAGCGTCCTGGGCACAGGAAGATCAGCAGGAT GCAGACGAAGACAGGCGAGCTTTCCAGATGCTAAGAAGAGATAATGAAAAAATAGAAGCTTCACTGAGAC GATTAGAAATCAACATGAAAGAAATCAAGGAAACTTTGAGGCAGTATTTACCACCAAAATGCATGCAGGA GATCCCGCAAGAGCAAATCAAGGAGATCAAGAAGGAGCAGCTTTCAGGATCCCCGTGGATTCTGCTAAGG GAAAATGAAGTCAGCACACTTTATAAAGGAGAATACCACAGAGCTCCAGTGGCCATAAAAGTATTCAAAA AACTCCAGGCTGGCAGCATTGCAATAGTGAGGCAGACTTTCAATAAGGAGATCAAAACCATGAAGAAATT CGAATCTCCCAACATCCTGCGTATATTTGGGATTTGCATTGATGAAACAGTGACTCCGCCTCAATTCTCC ATTGTCATGGAGTACTGTGAACTCGGGACCCTGAGGGAGCTGTTGGATAGGGAAAAAGACCTCACACTTG GCAAGCGCATGGTCCTAGTCCTGGGGGCAGCCCGAGGCCTATACCGGCTACACCATTCAGAAGCACCTGA ACTCCACGGAAAAATCAGAAGCTCAAACTTCCTGGTAACTCAAGGCTACCAAGTGAAGCTTGCAGGATTT GAGTTGAGGAAAACACAGACTTCCATGAGTTTGGGAACTACGAGAGAAAAGACAGACAGAGTCAAATCTA CAGCATATCTCTCACCTCAGGAACTGGAAGATGTATTTTATCAATATGATGTAAAGTCTGAAATATACAG CTTTGGAATCGTCCTCTGGGAAATCGCCACTGGAGATATCCCGTTTCAAGGCTGTAATTCTGAGAAGATC CGCAAGCTGGTGGCTGTGAAGCGGCAGCAGGAGCCACTGGGTGAAGACTGCCCTTCAGAGCTGCGGGAGA TCATTGATGAGTGCCGGGCCCATGATCCCTCTGTGCGGCCCTCTGTGGATGAAATCTTAAAGAAACTCTC CACCTTTTCTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213152 representing NM_152649
Red=Cloning site Green=Tags(s) MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALE EANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQD ADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLR ENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFS IVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGF ELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKI RKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_152649 |
ORF Size | 1413 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152649.4 |
RefSeq Size | 2496 bp |
RefSeq ORF | 1416 bp |
Locus ID | 197259 |
UniProt ID | Q8NB16 |
Domains | pkinase |
Protein Families | Druggable Genome, Protein Kinase |
MW | 54.3 kDa |
Gene Summary | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015] |
Citations (6)
The use of this cDNA Clones has been cited in the following citations: |
---|
Skp2-mediated MLKL degradation confers cisplatin-resistant in non-small cell lung cancer cells
,null,
Communications Biology
,PubMed ID 37532777
[MLKL]
|
Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
,null,
Cell Death and Differentiation
,PubMed ID 34999730
[MLKL]
|
Mixed Lineage Kinase Domain-Like Protein Promotes Human Monocyte Cell Adhesion to Human Umbilical Vein Endothelial Cells Via Upregulation of Intercellular Adhesion Molecule-1 Expression
,Cai, F;Wang, JL;Wu, YL;Hu, YW;Wang, Q;,
Med. Sci. Monit.
,PubMed ID 32788571
[MLKL]
|
MLKL Aggravates Ox-LDL-Induced Cell Pyroptosis via Activation of NLRP3 Inflammasome in Human Umbilical Vein Endothelial Cells
,Wu, Q;He, X;Wu, LM;Zhang, RY;Li, LM;Wu, CM;Lu, YB;Hu, B;Shi, C;Lu, ZF;Yang, B;Zheng, L;Hu, YW;Wang, Q;,
Inflammation
,PubMed ID 32621119
[MLKL]
|
The role of the LncRNA-FA2H-2-MLKL pathway in atherosclerosis by regulation of autophagy flux and inflammation through mTOR-dependent signaling
,Guo, FX;Wu, Q;Li, P;Zheng, L;Ye, S;Dai, XY;Kang, CM;Lu, JB;Xu, BM;Xu, YJ;Xiao, L;Lu, ZF;Bai, HL;Hu, YW;Wang, Q;,
Cell Death Differ.
,PubMed ID 30683918
[MLKL]
|
ATP-Competitive MLKL Binders Have No Functional Impact on Necroptosis
,Ma, B;Marcotte, D;Paramasivam, M;Michelsen, K;Wang, T;Bertolotti-Ciarlet, A;Jones, JH;Moree, B;Butko, M;Salafsky, J;Sun, X;McKee, T;Silvian, LF;,
PLoS ONE
,PubMed ID 27832137
[MLKL]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213152L1 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC213152L2 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
RC213152L3 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC213152L4 | Lenti ORF clone of Human mixed lineage kinase domain-like (MLKL), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
RG213152 | MLKL (tGFP-tagged) - Human mixed lineage kinase domain-like (MLKL), transcript variant 1 |
CNY 7,088.00 |
|
SC108101 | MLKL (untagged)-Human mixed lineage kinase domain-like (MLKL), transcript variant 1 |
CNY 5,488.00 |