NUP214 (AJ420413) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAIN; CAN
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AJ420413, the custom clone sequence may differ by one or more nucleotides GGCACGAGGGCAGCAGCAACACCACATCCTTCGGCACGCTCGCGAGTCAGAATGCCCCCACTTTCGGATC ACTGTCCCAACAGACTTCTGGTTTTGGGACCCAGAGTAGCGGATTCTCTGGTTTTGGATCAGGCACAGGA GGGTTCAGCTTTGGGTCAAATAACTCGTCTGTCCAGGGTTTTGGTGGCTGGCGAAGCTGAGGGCGTGTCA GCAGGCCTTTCGATCCCTGGGACCAACCGCATCCTCAGCTTCTTCCCCGAGAAATGCTGGAGCAGGCTGT TCAGACCGACGTTGCCATCAAAACACATACACCCAGAAAGAAACAACAGAAACCAAAACTCACAAGGCGC ATGATTACTTGTTTTATATTTCATGTTGGGTTTTCCCTCCCACTATTAAACAGTCTGTTTCCGTACAGAA CGTATGTGGGTTTTTTCAGATCACAGCCAAGAAGATTGCCCCGTTTCTGTGGCTCTGAGAAGCCAGCAGA GCCTCCATCAGCCAGAACACTGTGTCTTCAAGGATGGCATCAGAAGTCACCAGCGTCGGGTGTTGATAAA CAGCATCGAATGTGCCGTGGTCTCACTTGGACCTAGCGCCCTCCACATAGGGAAGAGGTTGAATGCTGGT GGGTCATCTTTTTGAGGCTGAAACTTGGTTATCTCCTCTCCTTGTTCTTTTAGCCATTGTGTATCAGGAC CATCCAAGGACGCACTCTGCGATTGAGTGGAGGCAGAGGAAGCCACTCATGCCAGCAGCAGTTGAGTTTC AGAAGCAGCCATAGCGCTTTTCAGTACAGTACAATAGTAGCCAGCGTGAGGCAGGACAGTGCTGCGCCTT AGCCGGCCTTGGCTCTCATCTCCATGTGGCTCTTGGGTGCCATGTGTCCTACTCCAGAGAGGAGGGGGCC GGTCACTTGAGAAGACAGCCTTTATATTCTGTATGTGGCAGTGACATTGGTAGCCACACCTACCCAGTGT CTACTAACCACAGTGCAGATAGCCCTTGGGTAGACCCAGGAAGTGTGAAGACAGACACTGCTGTTGCCAG ACAACACTAGCAGAACGATGCTGGATTTGACAACAGTGTTTGCTAGGACGGCCCAGAGCTCATCACTGCA GTTTTATTAGAGTGCTTCTTTATCTTTAATGCAGTATCTTTGAGGCCTGTAACATCCTAGATAGTTGCCA TCAAACAAGGACCGTCTCGCATTGAAAACAGATGTAAGAATTTCTCTTTCAGGCCCAGAATCTGACAGTG CTTTGGCTTGGGTCATGAGCAGCGGGGAGTTGGGAAGAGATTTTTTTTTTTAGAGTTTTACATATTGGCA GGTTGTATTTTTTTAATGTTTTAATAAAAGTTTGACATGACATTAATTCATCAAAAAAAAAAAAAAAAAA AAAAAAAAAAACGAAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AJ420413 unedited ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTGGCAGCCC TCCTACTTTTGGGGGATCCCCTGGGTTTGGAGGGGTGCCAGCATTCGGTTCAGCCCCAGC CTTTACAAGCCCTCTGGGCTCGACGGGAGGCAAAGTGTTCGGAGAGGGCACTGCAGCTGC CAGCGCAGGAGGATTCGGGTTTGGGAGCAGCAGCAACACCACATCCTTCGGCACGCTCGC GAGTCAGAATGCCCCCACTTTCGGATCACTGTCCCAACAGACTTCTGGTTTTGGGACCCA GAGTAGCGGATTCTCTGGTTTTGGATCAGGCACAGGAGGGTTCAGCTTTGGGTCAAATAA CTCGTCTGTCCAGGGTTTTGGTGGCTGGCGAAGCTGAGGGCGTGTCAGCAGGCCTTTCGA TCCCTGGGACCAACCGCATCCTCAGCTTCTTCCCCGAGAAATGCTGGAGCAGGCTGTTCA GACCGACGTTGCCATCAAAACACATACACCCAGAAAGAAACAACAGAAACCAAAACTCAC AAGGCGCATGATTACTTGTTTTATATTTCATGTTGGGTTTTCCCTCCCACTATTAAACAG TCTGTTTCCGTACAGAACGTATGTGGGTTTTTTCAGATCACAGCCAAGAAGATTGCCCCG TTTCTGTGGCTCTGAGAAGCCAGCAGAGCCTCCATCAGCCAGAACACTGTGTCTTCAAGG ATGGCATCAGAAGTCACCAGCGTCGGGGTGTTGATAAACAGCATCGAATGGTGCCGTGGT CTCACTTGGACCTAGCGCCCTCCCATANGGAAAAGGNTGAATGCTGGTGGGTCATCTTTT TGAGGCTGANACTTGGNTATCTCCTCTCCTTGNTCTTTTACCCATGGNGTATCAGACATC CAGGACGN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AJ420413 unedited GTAACAGCTATGNACCGCGGCACGCATCCTAGTATACGAGTTTTTTTTTTTTTTTTTTGA ATTAATGTCATGTCAAACTTTTATTAAAACATTAAAAAATACAACCTGCCAATATGTAAA ACTCTAAAAAAAAAAAAAATCTCTTCCCAACTCCCCGCTGCTCATGACCCAAGCCAAAGC ACTGTCAGATTCTGGGCCTGAAAGAGAAATTCTTACATCTGTTTTCAATGCGAGACGGTC CTTGTTTGATGGCAACTATCTAGGATGTTACAGGCCTCAAAGATACTGCATTAAAGATAA AGAAGCACTCTAATAAAACTGCAGTGATGAGCTCTGGGCCGTCCTAGCAAACACTGTTGT CAAATCCAGCATCGTTCTGCTAGTGTTGTCTGGCAACAGCAGTGTCTGTCTTCACACTTC CTGGGTCTACCCAAGGGCTATCTGCACTGTGGTTAGTAGACACTGGGTAGGTGTGGCTAC CAATGTCACTGCCACATACAGAATATAAAGGCTGTCTTCTCAAGTGACCGGCCCCCTCCT CTCTGGAGTAGGACACATGGCACCCAAGAGCCACATGGAGATGAGAGCCAAGGCCGGCTA AGGCGCAGCACTGTCCTGCCTCACGCTGGCTACTATTGTACTGTACTGAAAAGCGCTATG GCTGCTTCTGAAACTCAACTGCTGCTGGCATGAGTGGCTTCCTCTGCCTCCACTCAATCG CAGAGTGCGTCCTTGGATGGTCCTGATACCACATGGCTAANAGAACCAGGAGAGGAGATA ACCAAGTTTCAGCCTCAAAAAGATGACCCACCAGCATTCAACCTCTTCCCTATGTTGGAG GGCGCTAGGTCCCAGTGAGACCACGGCACAATTCGATGCTGTTTATCACCCCCCGACGCG
Restriction Sites	NotI-NotI
ACCN	AJ420413
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AJ420413.1
RefSeq Size	1416 bp
RefSeq ORF	1416 bp
Locus ID	8021
Gene Summary	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

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