ANO9 (AK026923) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PIG5; TMEM16J; TP53I5
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026923, the custom clone sequence may differ by one or more nucleotides AAACCCCAGCTGGAACTCCGGCCACAGCCAGCATGCAGGGCGAAGAGAGCCTCCGGATCCTGGTGGAGCC CGAAGGGGACAGCTTCCCGCTGATGGAGATCAGCACCTGTGAGACCGAGGCCTCCGAGCAGTGGGACTAT GTCCTCGTGGCCCAACGTCACACCCAGAGAGACCCCCGGCAGGCGCGGCAGCAACAGTTCCTGGAGGAGC TCAGGAGAAAGGGCTTCCACATTAAGGTGATCCGGGACCAGAAACAGGTCTTCTTTGGGATCCGTGCTGA CAACAGTGTCTTTGGCCTGTACCGCACTCTCCTCCTGGAGCCTGAGGGGCCTGCCCCCCACGCCGAGCTG GCCGCGCCGACCACCATCCCGGTCACCACGAGTCTCAGAATCCGAATCGTGAACTTCGTTGTCATGAACA ACAAGACCTCGGCTGGTGAGACCTTCGAGGATCTGATGAAGGACGGGGTCTTTGAGGCCAGGTTCCCCCT GCACAAGGGGGAGGGACGCCTGAAGAAGACGTGGGCGCGGTGGAGACACATGTTCCGGGAGCAGCCAGTT GATGAAATCAGGAACTACTTTGGGGAAAAGGTGGCCCTGTACTTCGTCTGGCTGGGCTGGTACACCTACA TGCTGGTGCCGGCCGCCCTGACGGGCCTCTTAGTCTTTCTGAGCGGATTCTCGCTGTTTGAGGCCAGCCA GATCAGCAAGGAGATCTGTGAGGCCCACGACATCCTCATGTGTCCCCTCGGCGACCACAGCCGCAGGTAC CAGCGGCTCTCGGAAACCTGCACTTTTGCCAAGCTCACCCACCTCTTTGACAATGATGGCACGGTGGTGT TCGCCATCTTCATGGCTCTCTGGGCCACGGTGTTCCTGGAGATCTGGAAGCGGCAGCGCGCCCGCGTGGT CCTGCACTGGGACCTGTACGTGTGGGACGAGGAACAGGAGGAAATGGCACTTCAGCTCATTAACTGCCCC GACTACAAGCTCCGGCCATACCAGCACTCCTACCTACGCAGCACCGTCATCCTCGTCCTGACCCTGCTCA TGATCTGCCTCATGATCGGCATGGCCCACGTCCTGGTGGTCTACCGCGTCCTGGCCTCCGCGCTCTTCAG CAGCTCGGCCGTGCCCTTCCTGGAGGAGCAGGTGACCACGGCCGTGGTGGTGACCGGGGCTCTGGTGCAC TATGTGACCATCGTCATCATGACCAAGATCAACAGGCGCGTGGCCCTGAAGCTTTGTGACTTCGAGATGC CCAGGACCTTCTCGGAGCGAGAGAGCAGGTTCACCATCCGCTTCTTCACACTGCAGTTCTTCACCCATTT CTCGTCTCTCATCTACATCGCCTTCATCCTGGGCAGGATCAACGGCCACCCCGGGAAGTCCACGCGCCTG GCGGGCTTGTGGAAGCTGGAAGAGTGCCACGCCAGCGGCTGCATGATGGACCTCTTCGTGCAGATGGCCA TCATCATGGGCCTGAAGCAGACGCTCAGCAACTGCGTCGAGTACCTGGTCCCGTGGGTGACCCACAAGTG CCGCTCTCTGCGGGCCTCCGAGTCCGGGCACCTGCCCCGGGACCCCGAGCTCAGGGACTGGCGGCGCAAC TACCTTCTGAACCCGGTCAACACCTTCAGCCTGTTCGACGAGTTCATGGAGATGATGATCCAGTACGGCT TCACCACCATCTTCGTGGCCGCCTTCCCGCTGGCGCCGCTGCTCGCGCTCTTCAGCAACCTCGTGGAGAT CCGCCTGGACGCCATCAAGATGGTCTGGTTGCAGCGGCGCCTGGTGCCGCGCAAGGCCAAGGACATCGGG ACCTGGCTGCAGGTGCTGGAGACCATCGGTGTGCTGGCGGTCATTGCCAATGGGATGGTCATTGCCTTCA CATCTGAGTTCATCCCCCGAGTGGTCTACAAGTACCGCTATAGCCCATGCCTGAAAGAAGGCAACTCTAC TGTCGACTGCCTCAAGGGCTACGTCAACCACAGCCTGTCCGTCTTCCACACCAAGGACTTCCAGGACCCT GATGGGATTGAGGGCTCAGAAAACGTGACTCTGTGCAGATACAGGGACTACCGCAATCCCCCCGATTACA ACTTCTCCGAGCAGTTCTGGTTCCTCCTGGCCATCCGCCTGGCCTTCGTCATCCTCTTTGAGCACGTGGC CTTGTGCATCAAGCTCATCGCCGCCTGGTTCGTGCCCGACATCCCTCAGTCGGTGAAGAACAAGGTTCTG GAGGTGAAGTACCAGAGGCTGCGTGAGAAGATGTGGCATGGAAGGCAGAGGCTGGGTGGGGTGGGGGCAG GCTCTCGGCCCCCAATGCCTGCCCATCCCACCCCAGCATCCATCTTCAGTGCCAGGAGCACAGACGTGTA GGGCCAGAGCCCGTCCAGAGGCCACCAGGAGCTGAGACAGTGCCACCACCAGCACCTCCCACAAGCCCAC CGCTGTGCGTGTTGAGGGGTGCTGTGAGAAGGCTGTGCCCATGTGGGGCCGCAGGAATCCCCTGTATGTT CAGGGCTGTGAGCTGCCACCCTATTCCGCCTGCTCCGTCTTTGTGGGGGCTCTCAGGCTTGGCACAGCCC TGACTTGAACTCTGGGTGACCCTGGGCACCCACAGAACTGGGAGTGAGGGCTCCTCAGGCAGCCACAAGG CAGGAAAACTGGCGCAAATTTCCTGGGCCTCCCTCTGACTTCTGGGCGCCAGATCCTGCCGTGCCCCCAT CCTGGCTGTTGGGGGTGTCCTGAGCCCACCTCGCTGGCCTGTTCCCTTCAGCCAACCCGTTTCTGCAGTA AAATTAAGCCTGTCAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026923 unedited AGTATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGAGCACCCG CCGCTGGCAGCCACTCTCCTGGGCGCCAAACCCCAGCTGGAACTCCGGCCACAGCCAGCA TGCAGGGCGAAGAGAGCCTCCGGATCCTGGTGGAGCCCGAAGGGGACAGCTTCCCGCTGA TGGAGATCAGCACCTGTGAGACCGAGGCCTCCGAGCAGTGGGACTATGTCCTCGTGGCCC AACGTCACACCCAGAGAGACCCCCGGCAGGCGCGGCAGCAACAGTTCCTGGAGGAGCTCA GGAGAAAGGGCTTCCACATTAAGGTGATCCGGGACCAGAAACAGGTCTTCTTTGGGATCC GTGCTGACAACAGTGTCTTTGGCCTGTACCGCACTCTCCTCCTGGAGCCTGAGGGGCCTG CCCCCCACGCCGAGCTGGCCGCGCCGACCACCATCCCGGTCACCACGAGAATCCGAATCG TGAACTTCGTTGTCATGAACAACAAGACCTCGGCTGGTGAGACCTTCGAGGATCTGATGA AGGACGGGGTCTTTGAGGCCAGGTTCCCCCTGCACAAGGGGGAGGGACGCCTGAAGAAGA CGTGGGCGCGGTGGAGACACATGTTCCGGGAGCAGCCAGTTGATGAAATCAGGAACTACT TTGGGGAAAAGGTGGCCCTGTACTTCGTCTGGCTGGGCTGGTACACCTACATGCTTTTGC CGGCCGCCCTGACAGGCCTCTTAGTCTTTCTGAGCGGATCCTCGCTGTTTGAGGCCACCC GATCAGCAAGGAGATCTGTGAAGCCCCGACATNCTCATGTGTTCCCTCTGNGACACAGCC GCAAGTACCAACGGCTCTCGGAAACCTGCACTTTGGCAGCTTACCCACCTTTTGACAATG AGGACGGGGGTGTTCCCCATTTCATGGTTTTTGGGGCACGGGTTCCTGAGATCTGAAACG CGACCCGCCCCTTGGCCTTCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK026923 unedited NGGAAATTAGCTAGTGTACCGTCGGCCGCAATCGAGGATCGAGTTAAATTCTTTTTTTTT TTTTTTTTTTTTTTTTTGACAGGCTTAATTTTACTGCAAAAACGGGTTGGCTGAAGGGAA CAGGCCAGCGAGGTGGGCTCAGGACACCCCCAACAGCCAGGATGGGGGCACGGCAGGATC TGGCGCCCAAAAATCAAAGGGAGGCCCAGGAAATTTGCGCCATTTTTTCTGCCTTGGGGC TGCCTGAGGAGCCCTCACTCCCAGTTCTGTGGGGGCCCAGGGGCACCCAGAGTTCAAGTC AGGGCTGTGCCAAGCCTGCGAGCCCCCACAAAGACCGAGCAAGCGGAATAAGGGGGCAGC TCACAGCCCTGAACATACAGGGGATTCCTGCGGCCCCACATGGGCACAGACTTTTTACAG AACCCCTCAACACGCACAACGGTGGGCTTGTGGGAAGTGCTGGTGGTGGCACTGTCTCAA CTTCTGGTGGCCTCTGGACGGGCTCTGGGCCTAAAAGTCTGTGCCCCCGGCACTGAAAAT GGTGCCCCAAAACCTTGGTTTTCACCCACTGAGGGATGGTCGGCACCAAAACAGCGGCGA ATAACTTGATCCCAAAGGCCCCTGCCCTAAAAAGAAGAAAAAAAGCCCACCCGGAGGGCC CCGAAGAACACCAAACCGCTCCGAAAAAGATTTATATCGGGCGGATATGGGGGGGGCCCC GGAATTGGACAAAAAACAATTTTTTGGACCCTCAAACCCCCCAGGGCCCTGGAAATCTTT GGGGGGAAAAAAGAAAGCGGGGGTGAAAAAACCCTTGGGGGACCAAGAAAAAATTGCTCC TTTAGGAGATGGGGAAACGGGGCCTGTGAAACACTGGGGGAAGAACACCAAG
Restriction Sites	NotI-NotI
ACCN	AK026923
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026923.1
RefSeq Size	2831 bp
RefSeq ORF	2831 bp
Locus ID	338440
Domains	DUF590
Protein Families	Druggable Genome, Transmembrane
Gene Summary	The protein encoded by this gene is a member of the TMEM16 (anoctamin) family of proteins, some of which form integral membrane calcium-activated chloride channels. The function of the encoded protein has yet to be elucidated, although it may have channel-forming abilities and also may have phospholipid scramblase activity. This gene has been observed to be upregulated in stage II and III colorectal cancers. [provided by RefSeq, Dec 2016]

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