LOC285498 (RNF212) (AK096160) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZHP3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096160, the custom clone sequence may differ by one or more nucleotides ATGGCCAACTGGGTGTTCTGTAATCGCTGCTTCCAGCCGCCCCACAGGACGTCGTGCTTCAGCCTCACCA ACTGCGGGCACGTGTACTGCGACGCCTGCCTCGGCAAAGGTAAAAAGAATGAATGCTTGATTTGTAAAGC TCCTTGTCGTACAGTTTTGCTTTCAAAGCATACCGACGCAGATATCCAGGCATTCTTCATGAGCATAGAC AGTCTGTGTAAGAAGTACTCCAGGGAAACCTCCCAGATTTTAGAATTTCAAGAAAAACACAGGAAGAGAT TGTTAGCCTTCTATAGAGAAAAGATTTCTAGGTTGGAAGAATCCCTTAGGAAGTCAGTGCTGCAGATAGA ACAACTACAAAGTATGAGATCATCACAACAAACAGCTTTCAGCACAATAAAAAGTTCAGTTTCAACAAAA CCACACGGATGCCTGCTGCCACCTCACTCATCAGCCCCCGACAGACTGGAGTCGATGGAAGTTGATCTCT CTCCTTCTCCGATTAGAAAATCTGAGATAGCAGCCGGCCCTGCGAGAATCTCCATGATTAGTCCACCTCA AGATGGACGAATGGCCCCCTGTGCCCGGAGAGTGTGTCATTTCCAGAGGTTCACCATGTTTCTGCATAGA CGTCTGTCCTCACTGGCTGCTCCTCCTAGCGTTCAGTTCTGGAAGGCACGGGGAACTCACCAACTCTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096160 unedited CAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCTTGCGTGGGCTT CGCGTGGGCCCGGCCTCGCCGCTGCGGTCGCCCGCCTGGCATGGCCAACTGGGTGTTCTG TAATCGCTGCTTCCAGCCGCCCCACAGGACGTCGTGCTTCAGCCTCACCAACTGCGGGCA CGTGTACTGCGACGCCTGCCTCGGCAAAGACCGACGCAGATATCCAGGCATTCTTCATGA GCATAGACAGTCTGTGTAAGAAGTACTCCAGGGAAACCTCCCAGATTTTAGAATTTCAAG AAAAACACAGGAAGAGATTGTTAGCCTTCTATAGAGAAAAGATTTCTAGGTTGGAAGAAT CCCTTAGGAAGTCAGTGCTGCAGATAGAACAACTACAAAGTATGAGATCATCACAACAAA CAGCTTTCAGCACAATAAAAAGTTCAGTTTCAACAAAACCACACGGATGCCTGCTGCCAC CTCACTCATCAGCCCCCGACAGTCTGAGATAGCAGCCGGCCCTGCGAGAATCTCCATGAT TAGTCCACCTCAAGATGGACGAATGGCCCCCTGTGCCCGGAGAGTGTGTCATTTCCAGAG GTTCACCATGTTTCTGCATAGACGTCTGTCCTCACTGGCTGCTCCTCCTAGCGTTCAGTT CTGGGAGGCACGGNGAACTCACCAACTCTAAAACACTTCCAATATATGCTGAGGTACAAA GGGCCGTCTTGTTTTCGTTCCAGCAGGCTGAGGGCACCCTGNACACGTTCAGACACCCGC TGGGTCTGGTGTGTTTCCTCTTTGCCAATTTGAAAGGGAAAAGTCATTTTGATTNGCATT TATGTGACTATTAATGATTCTTGATGGTTTCAATTATTCCTCTGGGGTTTTTCTACAAAT ATCTGCTTATTCCCTTGTCATTATGGGGAGCCCTCATGTGCAGATATAACCTTTACCCNA TTGCTTGNATTTTAATTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK096160 unedited AAAATACTGTGTTANCGCGGCCGCATTTCTAGATCGCATTTTTTCTTTTTTTTTTTATAT TTTGCAATTTCATATTTATCTGATAAGGAAATGTCATCCAAAAAAAAGAACACAAAAAAG AAATGTCATCCAAAATATACACAGGACTCTTAAAACTCAACAATAAGAAAACAATCCAAT TAAAAAATGGGCCAAATACCTTAACAGACACTTCAGCAAAGAAGATATGCAGATGAAAAA TCAGCATAAGATGCTCCTAGTCATATGTTATCAATGAAATGCAAAATAAAACAGCAAGAT ACCACTACATACCTATCAGCTGGCCAAAATCCAGAACACTGACATCATCAAATGTGAGGA CGTGGAGCAACAGGAACTCTCATTCGTTGCTAGTGGGAATGCGAAAAGCTGCTGCCACCC TGGAAGGTAGCTCGGCAATTTCCTTCAAAACTAAACACCCTCTCACCATCTGATCCAGCA TTCACACTCCTTACTATTTACCCAAAGGAGCTGAAAACATGTCTACACACGAACCCTGGC ACATGGACATCTATGGCAACCTAAATCATAATTGCCAAAACTTGGAATCCACCAAGAGGC CCGTTCGAAGGGGAAGTGGAGAACTACGAGCCACGCGCGAGATGGCATATCATTCAGTGG CCTGAAAAAATGAGCTAGCCCACCACGAAAAGAAAGGGGGGGAACCAGATATGTGGATTG GATAGTTAAAAGGAGTGATAATGAAGAAGCGAGAAAAGAGAAATGTCGAGCGTCGGAAGG ATTTCGAAAGAGGGCCGACAACAAAAAAAGTGCCTATCAAGGTTGGGTGGAAAAAGGGAA CAAATAAAGGGAAGGGTGCGCGGCGGTACGGGCTCCCCANCACCCCGTCCCGCTACCTCA GGCGCGCCCCCGCCGACCGCGG
Restriction Sites	NotI-NotI
ACCN	AK096160
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK096160.1, BAC04714.1
RefSeq Size	2333 bp
RefSeq ORF	699 bp
Locus ID	285498
Gene Summary	This gene encodes a RING finger protein that may function as a ubiquitin ligase. The encoded protein may be involved in meiotic recombination. This gene is located within a linkage disequilibrium block and polymorphisms in this gene may influence recombination rates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010]

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