AP4B1 (AK057567) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BETA-4; CPSQ5; SPG47
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK057567, the custom clone sequence may differ by one or more nucleotides GGTGCAGACTTTCCGAGACCTGGTTTGGTTGTGTCCTCAGTGTACTGAAGCTGTATGTCAGGCCCTGCCC GGCTGTGAAGAGAACATTCAAGATAGTGAGGTAGACTGTAATTCTCCTTTACTTCTAGGTAGGGGGAGAA TGCTTGAAACCCTGCCCTTTGAAATGGAATCTAATTATTCTCTCTACCCTGTGTTTGAAGTGGGCACTCT TCGAATTGATTGCCACCTTAGATTCTCTTTTGGATATAGATGGCATATAAATTATAAAAACAAGATTTAT CCGACGGGTTTTATTACAAAACTCTGATACCATTGTTCTCTTGAACAGTTCTGTTTTGTTTTGTTTTGCT TTGCTTGAGATAGAGTTGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCCCACTGCAACCTCTACCTCC CAAACTCAAGTGATTCTCTTGCCTCAGCCTCCTGAGTAGAGTAGCTGTGATTACAGGTGTGCACCACCAC GCCCAGCTAATTTTTGTATTTTTAGTAGAGAGGGTTTTGCTATGTTGTCTAGGCTGGTCTAGAATTCCTA GCCTCAAGTGATCACCCACCTCAGCATCCCAGAGAGCTAGGATTACAGGCATAAGCCACTGTGCCCCGCT TCTCTTGAACAGTTTTGAGTCAGAGATTTAGACCATGGAGTTTTTAGCTAGAATGAAAATTATAATCATT TGTGAAATTTTTACAAAGATTTGGACGTAATCACAGGTCTACTGACTTAAATATTCAGGGGTGTTGGACC TAGACAGATAACTTTTTTTTTTTTTTTTTTTTTGAAACAGAGTCTAGCTCTGTTGCCCAGGCTGGAGTGC AGTAATGCCATCTTAGCTCACTGCAACTTCCGCCTCCTGGTTTCAAGCAATTCTCCTGCCTCAGGCTCCC GAGTAGCTGGAACTACAGGTGTGCACCACCACGCCTGGCTAATTTTTGTATTTTTAATAGAGACGGGTTT CACCATATTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCTCCTCGGCCTCCCAGAGTG CTGGGATTACAGACGTAAGCCACCGTGCCCGGCTGACAGATACATTTTTAAGAAGCTTCACAGATGACTC TGATGTGCACTCTAGGTTAGTAACCATTTTTGTGAAAAATTTAGAGAAATTCTTTGAGCAGCTTCCACTG AAACACTAAAACCCAATAGGGCCAAAGGCCCCAAACCTGAGGAAACCTTATTTATTGCTTAATCCAACAT AGGCTATGAAAGTTTTGAGTTTCCTCTTGTGTATTAGAATTTCATTCCTATTTGTTGTAGAGAGTATAGT ACGGGGAATCAGTAAATTAAATGAAGTAAACTAAAGATTACACCTTTTCTGCTGGCACTAAGCGAAAAGC AAAACCAGTGGCTGTCTATTATCTCCTGTGTTTTAGACAGTCAAGTGTTGAGCACTTGCTCTTATTCCTT TATTCTCTAGGGGAAGCAAGCACTTATTTGGCTACTTGGTGTCCATGGGGAAAGAATTCCTAATGCTCCT TATGTGTTAGAGGACTTTGTTGAGAATGTGAAGTCGGAAACATTTCCAGCTGTTAAGATGGAGCTGCTCA CTGCTTCGCTGCGCCTTTTCCTCTCCCGACCTGCTGAGTGCCAGGACATGCTAGGACGTTTGTTGTATTA CTGCATAGAGGAAGAAAAAGATATGGCTGTACGGGACCGAGGTCTCTTCTATTATCGCCTCCTCTTAGTT GGCATTGATGAAGTTAAGCGGATTCTGTGTAGCCCTAAATCTGACCCTACTCTTGGACTTTTGGAGGATC CGGCAGAAAGACCTGTGAATAGCTGGGCCTCAGACTTCAACACACTGGTGCCAGTGTATGGCAAAGCCCA CTGGGCAACTATCTCTAAATGCCAGGGGGCAGAGCGTTGTGACCCAGAGCTTCCTAAAACTTCATCCTTT GCCGCATCAGGTAAAAACAGTCCTTACCTTAAATCTTGTCATGATAAATCTTTACCTTTTCAATGATTGG TGGAAAGTAGAGTATCTTAGCACTAAACCTCAGACTGTTGCCTGAATTTGAAGCATTTGTGAGCAAGAAA GAGGTTCGTCACTGGTTATTTCAGCCATTTGTCCAAAAATAAAAGTCACATTGTGTACATTTGGGAATTT GGATAAAAAACTTAGCTCCTACAGATTAAGAACATCTTTCACACAAGTTTTCTGTCTCTTTTAGGACCCT TGATTCCTGAAGAGAACAAGGAGAGGGTACAAGAACTCCCTGATTCTGGAGCCCTCATGCTAGTCCCCAA TCGCCAGCTTACTGCTGATTATTTTGAGAAAACTTGGCTTAGCCTTAAAGTTGCTCATCAGCAAGTGTTG CCTTGGCGGGGAGAATTCCATCCTGACACCCTCCAGATGGCTCTTCAAGTAGTGAACATCCAGACCATCG CAATGAGTAGGGCTGGGTCTCGGCCATGGAAAGCATACCTCAGTGCTCAGGATGATACTGGCTGTCTGTT CTTAACAGAACTGCTATTGGAGCCTGGAAACTCAGAAATGCAGATCTCTGTGAAACAAAATGAAGCAAGA ACGGAGACGCTGAATAGTTTTATTTCTGTATTAGAAACTGTGATTGGAACAATTGAAGAAATAAAATCAT AACAGAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057567 unedited GGGGGTTCAACATTTGTNATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTGG AACTACAGGTGTGCACCACCACGCCTGGCTAATTTTTGTATTTTTAATAGAGACGGGTTT CACCATATTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCTCCTCGGCC TCCCAAGTGCTGGGATTACAGACGTAAGCCACCGTGCCCGGCTGACAGATACATTTTTAA GAAGCTTCACAGATGACTCTGATGTGCACTCTAGGTTAGTAACCATTTTTGTGAAAAATT TAGAGAAATTCTTTGAGCAGCTTCCACTGAAACACTAAAACCCAATAGGGCCAAAGGCCC CAAACCTGAGGAAACCTTATTTATTGCTTAATCCAACATAGGCTATGAAAGTTTTGAGTT TCCTCTTGTGTATTAGAATTTCATTCCTATTTGTTGTAGAGAGTATAGTACGGGGAATCA GTAAATTAAATGAAGTAAACTAAAGATTACACCTTTTCTGCTGGCACTAAGCGAAAAGCA AAACCAGTGGCTGTCTATTATCTCCTGTGTTTTAGACAGTCAAGTGTTGAGCACTTGCTC TTATTCCTTTATTCTCTAGGGGAAGCAAGCACTTATTTGGCTACTTGGTGTCCATGGGGA AAGAATTCCTAATGCTCCTTATGTGTTAGAGGACTTTGTTGAGAATGTGAAGTCGGAAAC ATTTNCAGCTGTTAAGATGGAGCTGCTCACTGCTNTGCTGCGCCTTTTCCTCTNCCGACC TGCTGAGTGCCAGGACATGCTANGACGTTTGNTGTATTACTGCATANGTGGGTTTTTCAG AAGGAAATAGTATTTGCCATGACCCATAGTAAAAATTCTTATAGCNTTCATTGNTGGNTG AGTCACGTCTGGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK057567 unedited GTTCATCTATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAAA CTCTGTTATGATTTTATTTCTTCAATTGTTCCAATCACAGTTTCTAATACAGAAATAAAA CTATTCAGCGTCTCCGTTCTTGCTTCATTTTGTTTCACAGAGATCTGCATTTCTGAGTTT CCAGGCTCCAATAGCAGTTCTGTTAAGAACAGACAGCCAGTATCATCCTGAGCACTGAGG TATGCTTTCCATGGCCGAGACCCAGCCCTACTCATTGCGATGGTCTGGATGTTCACTACT TGAAGAGCCATCTGGAGGGTGTCAGGATGGAATTCTCCCCGCCAAGGCAACACTTGCTGA TGAGCAACTTTAAGGCTAAGCCAAGTTTTCTCAAAATAATCAGCAGTAAGCTGGCGATTG GGGACTAGCATGAGGGCTCCAGAATCAGGGAGTTCTTGTACCCTCTCCTTGTTCTCTTCA GGAATCAAGGGTCCTAAAAGAGACAGAAAACTTGTGTGAAAGATGTTCTTAATCTGTAGG AGCTAAGTTTTTTATCCAAATTCCCAAATGTACACAATGTGACTTTTATTTTTGGACAAA TGGCTGANATAACCAGTGACGAACCTCTTTCTTGCTCACAAATGCTTCAAATTCAGGCAA CAGTCTGAGGTTTAGTGCTAAGATACTCTACTTTCCACCAATCATTGAAAAGGTAAAGAT TTATCATGACAAGATTTAANGGTAAGGGACTGTTTTACCTGATGCCGCAAAGGATGAAGT TTTANGAAGCTCTGGGTCACAACGCTCTGCCCCCTGGCATTTANAGATAGTTGCCCCATG GGCTTGGCATACACTGGCACCATTGGGTTGAAGTCTGAGGCCANCTATTCACAGGTCTTC TGGCGGATCCTCCAA
Restriction Sites	NotI-NotI
ACCN	AK057567
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK057567.1
RefSeq Size	2667 bp
RefSeq ORF	2667 bp
Locus ID	10717
Protein Pathways	Lysosome
Gene Summary	This gene encodes a subunit of a heterotetrameric adapter-like complex 4 that is involved in targeting proteins from the trans-Golgi network to the endosomal-lysosomal system. Mutations in this gene are associated with cerebral palsy spastic quadriplegic type 5 (CPSQ5) disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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