Nup214 (NM_001168559) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1304977
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217734 representing NM_001168559 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACGAGATGGATGCCATGATCCCTGAGCGGGAGATGAAGGACTTTCAGTTTAGAGCTCTGAAGA AAGTGAGAATCTTTGACTCTCCTGAGGAGTTGCCCAAGGAGCGCTCGAGTGTGCTCACCGTATCCAATAA GTATGGCATGCTCTTTGCTGGTGGCACCAATGGCTTGAATGTCTTTCCTACTAAAAGCCTTCTTATCCAA AACAAACCTGGAGATGATCCCAATAAAATTGTTGACACAATCCAAGGCTTGAATGTCCCTATGAAATTCC CAGTTCATCACCTGGCACTGAGCTGTGATAACCTTACACTCTCTGCGTGCATGATGTCCAGTGAATATGG CTCCATTATAGCCTTTTTTGATGTTCGCACATTTTCAAATCAGGCTAAACCACTGAAACGCCCATTTACC TATCACAAGCTTTCAAACGGTGCCAGCGGGATGGTGAATGATATGAAGTGGAACCCCACTGTCCCTTCCA TGGCAGCAGTTTGTCTGGCTGATGGCAGCATTTCTGTCTTGCAAGTTACGGATGTGGTGAAAGTGTGCGC CACACTTCCTCCTTCATCGGGAGTCACGTGTGTATGCTGGAGCCCCAAAGGAAAGCAGCTGGCCGTGGGA AAGCAGAATGGAACTGTGGTCCAGTATCTTCCTACATTGCAGGAAAAAAAGGTCATTCCATGTCCTCCGT TTTATGAGGCAGACCACCCTGTCAGAGTTCTGGATGTGCTGTGGATTGGTACATACGTCTTCACGATAGT CTATGCTGGTGCGGATGGCGCACTGGAGACCTGTCCAGATGTGGTGATGGCTCTGCTTCCGAAAAAAGAA GAAAAGCACCCAGAAATATTTGTGAACTTCATGGAGCCCTGCTACAGCAGCTGCACAGAGAGACAACATC ATTACTACCTCAGCTACATCGAGGAGTGGGATTTAGTGCTGGCAGCATCTGCAGCGTCAACAGAAGTTAG CATCCTTGCTCGACAAAGTGATCAGACTAATTGGGAATCTTGGGTACTGGAAGACTCTAGTCGAGCTGAA CTGCCTGTGACAGACAAGAGCGATGACTCCTTGCCCGTGGGTGTTGCCATAGATTACACCAATGAAGTAG AAGTTGCCATCAATGAAGAAAAGACTCTTCCTCCTGCTCCGGTTCTCCTGTTACTTTCAACAGATGGTGT ACTTTGTCCATTTTATATGATCAATCAAAATCCCGGGGTTAGGTCCCTAATCAAGACCTTAGAGCTGATC TCAGCAGAGGGAGAGCGCCAGCCTAAGTCCTCAGGAAGTTTTCCTAGTACACCACCTTCCCCTCAAGCTC CACAGAACCTTGACGCTCCAGCAACAGCCTCTTCGCCTCTTCCACCGGTGTCTGCTGCTCCCACCGCCAC CTTCCCTTTGCCGTCTGCTGGAGGAGGCCCTTCTGTGTTCTCCTTTGGCCCTTCACCCTTCAAGTCCTCC TCCATCCCCGGGGAGCCCCCGCCATACCCCACTGGCTCTGACAGTTCCAGAGCAGCACCAGCCTCTGGCA CCCCCACCTTCTCTTTTGCGCCTCCTTCCAAAGGCACCTTGGCCCCGACCCCTGCAGTGGCTCCTGTGCC CACATCTGCTGCTCCCTTCACTTTCGGATCATCAGGTTTTAAGCCCCCCTTGGAAAGCACACCTATGTCC AGTGCCCCAAACACAGGGATGAAGCCTTCATTCCCACCCTCAACTTCTTCGGTGAAAGTCAACCTGAACG AGAAGTTCACCGCTGTGGCCTCCTCTGCACCCGTTCATAGCTCCACAAGCATACCCTCCGTGTTGCCGTT CTCTTCCTCCCCCAAGCCACCAGCTTCTGGACCACTCAGCCATCCCACGCCTCTCCCAGCATCACCCAGC TCCATGCCTTTGAAACCTTCAGTCTCTCCCTCTCCGTCAGTTGGCCGCTCCGCCCAGACTGCCCCGAGTT CAGCACCCTCCACAGGGCAGAAATCTCCTAGGGTAAATCCTCCAGTGCCAAAGTCAGGGTCCTCTCAGGC CAGAGCACTTCAGCCTCCTGTTACAGAGAAGCAGAGGCATCAGTGGAAAGAGTCAGACCCTGTGATGGCT GGAATTGGAGAGGAGATTGCACACTTCCAGAAAGAGCTGGAGGAGTTAAAGGCGCGAACCTCCAAAGCCT GTTTCCAGGTCGGCACCCCTGAGGAGATGAAGATGCTGCGCACAGAGTCAGACGACCTGCACACCTTCCT CTTTGAGATCAGAGAGGCCACAGAGTCTCTTCATGGAGACATAAGTACACTGAAAACAACTCTGCTGGAG GGCTTTGCTGGTGTTGAAGAAGCCAGAGAACAGCATGGAAGAAATCATGACTCTGGGTATCTCCATCTGC TTTATAAAAGACCACTGGATCCCAAGAGCGAGGCCCAGCTCCAGGAAATTCGCCGCCTTCACCAGTATGT GAAATTTGCTGTCCAGGATGTGAATGATGTCCTAGACTTAGAGTGGGATCGGCATCTGGAGCAGAAGAAA AGACAGAGGCGCCTAATTGTGCCAGAGAGAGAGACACTTTTCAACACACTAGCCAACAACCGGGAAATCA TCAACCAGCAGAGGAAGAGGTTGAATCACCTGGTGGACAGTCTGCAGCAGCTCCGTCTCTATAACCAGAC GTCCCCATGGAACCGGCCCTCAACGCTTTCAACGCAAAGTGACACTCCTAGTTTTGACAGTGACCTTGAA CACCTGCTAAAGACTACCATTGAATCTCACACCAAGCCTTCTCCTAGAGTGCCAGGTAAACTGTCCCCTG CGAAACAGGCCCAACTGAGGAACTTCTTGGCCAAAAGGAAGACCCCACCAGTGAGGTCTACTGCTCCAGC CAGCCTGTCTCGGTCAGCCTTCCTGTCTCAGAGGTACTATGAAGACTTAGATGAAGGCAGCTCCGCGTCG TCCGTAGCTCACCCTCTGGAGGGAGAAGATGCCCGGCCGACGCCGACCTGCAGAGAGGAGGAGGCTGTGG TCCCAGTCCCTCGGCATGCCCCCGTGGTCCGCACACCTTCTGTCCAACCCAGTCTCCTGCCTCAGGCGAT GCCTTTTGCAAAGCCTCACCTGATTCATAGTTCTTCACCTGCTGCGATGAGCTCTTCAATCTCTACGTCA GCTACCAAAGTTATTCCTCAAGGAGCCGACACCACAATGCTGGCAACTAAAACGGTAAAGCATGGTGCAC CAGGTCCTTCCCACACTGTCGCAGCTCCCCAGGCAGCGGCTGCTGCAGCCCTGAGACGGCAGATGGCCAG TCAGGCGCCAGCTGTGAGCACTCTGACCGAATCCACTTTGAAGAATGTCCCTCAAGTGGTGAATGTACAG GAACTGAAGAGTAACCCCTCACCCCCTTCTGCGGCCATGGGTTCTTCTGGGCCGCACTCAGCAGCCAAAG CCCCCCATGCAGTGTTAACCCCAGTGGCTGCCAGCCAAGCCAAGCAGGGGTCTCTGATAAATTCCTTCAA GCCGTCAGGACCCACACCAGTGTCCTGTCAGCTGTCCTCTGGGGATAAAGCTGTAGGTCCAGGGACGGCC AAGACAGAGTCACCTGTGACCTCCACCCCATCTACGGGGCAGCTCAACAAGCCTTTCTCATTCGCCTCCC CAGGGACCTTTACTTTTGGGACAATCACACCAGCCCCATCTTCTAGTTTCACTGCCACACCAGGGGCAGC ACCCCCCACTAAAGAGTCAAACCAGCTTGAGGCGTTCTCATTTGGCGGGGGAGGCAAACCTTTTTGTGAG ACCATTCCTGGAAACCCCCCTGCCACGGGACCCCCATCAGCACCCAGCACTTCTGTAACAACTACGTCTC TGGAAGACTCTGCTCCAACCAGCAGCAAACCTGCAACTCCTGAAACTCCTGTGTCCACTGCCTCTAGCAA ATTGGAAACTCCACCATCCAAGTTAGGAGAGCTTCTCTTCCCAAGTTCTCTAGCTGGAGAGACGCTAGGG AGCTTCTCAGGACTGCGTGTTGGCCAGGCTGAGGAGTCTACAAAGCCCGTTAGTAAGGCCTCCTCCACAA ACCTGGCCGGAGCCCAGCCAGCCAAGCCCTCAGGGTTTAGCTTTCCTGCTGCTTCCGTGTTAGGGAAGCC AGTGGAGCCCAGTGTGACGTCCTCTGCCCCAGCGGCACCAGTCTCAGCTCTGAGTGCGAGCACTAGCAGT TCCTCGGCAACTGTCTTTGGCAGTGTGCCCCTTACCAGTGCTGCACCTTCCGGGCTCATGAGTTTTAGTG GGGCCTCTTTGGCTAGTAGCAAAGCTAGTTTCTCATTTGGAAACCAACAGACTAGTAGTGCAGCAGCAGC AGCAGCTACCCCAGCATCCACCTCAGTTGCCCCTTCCCTTCCAGCATCTTTCCCTACTTTGTCATTTGGT GGCCTCCTGAGTTCACCATCAGCTTCTTCCCTGCCTGTGTCTTCTGGTAAGATCACAGAGGAGGCCACAC CACCAGCTGTGCCTGACAAGTCAGACCACAGTGAAGTGTCAGCATCAACACCCTCACTTCCACTACAGCC ACCGTCAGCCCAGCTTCCCCAGGCATCTCTGCAAACTTCTGACCCTGTCAAAAAAGAACCTGTTCCTGCG CAGACCACAGACAGCAGACCATCATCTTCTGCCAGCCTTGTGGCATCCACAGAATCCACTCCAGTCGCCC CAGGGGGCCCTGATAGCAAGACAGAAGCAGTGTCCCCTGCTTCCACCTTTGCAGGGCCTGGGCCGGCCGT GGTTGCAGCTGTTCTCCTAGGAGCAGGCTCGGCAGCCAGTGAAGCTTCCGGTACCCCTAGTACCTCCAGC ACTGTCTCCAGCAGTACCCCAACTCCTGCCACAGAGACAGCTGTATTTGGGACTGCCACTTCTGGCTCCT CAGTCTTTACACAGCCGCCTGCTGCCAGTTCGAGCTCAGCATTCAACCAGCTCTCCAGTAACACGGCCAC TGCACCCTCCGCCACCCCCGTGTTTGGGCAAGTGGCAGCCAGCATAGCCAGCACAGCCGCTGCCACGCCA CAGGCCAGCAGTTCAGGATTTGGCAGCCCTGCGTTTGGTGCTTCGGCTCCTGGAGTGTTCGGACAGACAG CATTTGGGCAGTCCCCAGCCTTTGGGCAAGCAACAAGCAGCCCTGCGAGTGGCTTCTCCTTCAGCCAACC TGGGTTCAGCTCTGTCCCTGCTTTTGGCCAGTCTGTCTCCTCCACCCCCACATCCACCAGTGCCAATGTC TTCGGAGCCACCTCAAGTACCAGCAGCCCTGGTTCCTTCTCATTTGGACAGGCTTCTACCAATACAGGAG GGACGCTATTTGGCCAAAACAACCCTCCTGCTTTTGGTCAGAGCCCGGGCTTTGGGCAGGGAGGCTCTGT GTTTGGTAGCACCTCGGCCACTACCTCTACAGCAGCACCTTCAGGGTTTAGCTTTTGTCAAGCCTCCGGT TTTGGGTCTAGTAATACTGGTTCTGTGTTTGGTCAGGCAGCCAGTAGCGGTGGGTCAGTCTTTGGCCAGT CATCCACTTCCAGTGGCAGCGTGTTTGGGTCTGGAAATGCTACAAGAGGGGGCGGTTTCTTCAGTGGCCT TGGAGGAAAACCCAGCCAGGATGCTGCCAACAAAAACCCATTCAGCTCGGCCAGTGGGGGCTTTGGGTCT ACGCCTACACCCAATACCTCTAACCTGTTCGGGAACAGCGGAGCTAAGACCTTCGGAGGGTTCGGCTCCT CCTTCGGGGAGCAGAAGCCTGCTGGCACATTCAGCTCTGGTGGTGGGAGCGTGGCATCCCAAGGTTTTGG ATTTTCCACTCCAAATAAAACAGGTGGCTTCGGTGCTGCTCCAGTGTTTGGCAGCCCTCCTACTTTTGGG GGATCCCCTGGGTTTGGAGGGGTGCCAGCATTCGGTTCAGCCCCAGCCTTTACAAGCCCTCTGGGCTCGA CGGGAGGCAAAGTGTTCGGAGAGGGTACTGCGGCCGCCAGCGCAGGAGGATTCGGGTTCGGGAGTAGCAG CAACACTGCATCCTTCGGTACCCTCGCCAGTCAGAACGCACCCACTTTCGGATCCTTGTCCCAGCAGACC TCTGGCTTCGGGACCCCGAGCAGTGGATTCTCTGGGTTTGGATCAGGCACAGGAGCATTCACCTTCGGAT CATCTAACTCGTCTGTCCAGGGCTTCGGTGGCTGGAGAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217734 representing NM_001168559 Red=Cloning site Green=Tags(s) MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSVLTVSNKYGMLFAGGTNGLNVFPTKSLLIQ NKPGDDPNKIVDTIQGLNVPMKFPVHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNQAKPLKRPFT YHKLSNGASGMVNDMKWNPTVPSMAAVCLADGSISVLQVTDVVKVCATLPPSSGVTCVCWSPKGKQLAVG KQNGTVVQYLPTLQEKKVIPCPPFYEADHPVRVLDVLWIGTYVFTIVYAGADGALETCPDVVMALLPKKE EKHPEIFVNFMEPCYSSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQTNWESWVLEDSSRAE LPVTDKSDDSLPVGVAIDYTNEVEVAINEEKTLPPAPVLLLLSTDGVLCPFYMINQNPGVRSLIKTLELI SAEGERQPKSSGSFPSTPPSPQAPQNLDAPATASSPLPPVSAAPTATFPLPSAGGGPSVFSFGPSPFKSS SIPGEPPPYPTGSDSSRAAPASGTPTFSFAPPSKGTLAPTPAVAPVPTSAAPFTFGSSGFKPPLESTPMS SAPNTGMKPSFPPSTSSVKVNLNEKFTAVASSAPVHSSTSIPSVLPFSSSPKPPASGPLSHPTPLPASPS SMPLKPSVSPSPSVGRSAQTAPSSAPSTGQKSPRVNPPVPKSGSSQARALQPPVTEKQRHQWKESDPVMA GIGEEIAHFQKELEELKARTSKACFQVGTPEEMKMLRTESDDLHTFLFEIREATESLHGDISTLKTTLLE GFAGVEEAREQHGRNHDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKK RQRRLIVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWNRPSTLSTQSDTPSFDSDLE HLLKTTIESHTKPSPRVPGKLSPAKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEGSSAS SVAHPLEGEDARPTPTCREEEAVVPVPRHAPVVRTPSVQPSLLPQAMPFAKPHLIHSSSPAAMSSSISTS ATKVIPQGADTTMLATKTVKHGAPGPSHTVAAPQAAAAAALRRQMASQAPAVSTLTESTLKNVPQVVNVQ ELKSNPSPPSAAMGSSGPHSAAKAPHAVLTPVAASQAKQGSLINSFKPSGPTPVSCQLSSGDKAVGPGTA KTESPVTSTPSTGQLNKPFSFASPGTFTFGTITPAPSSSFTATPGAAPPTKESNQLEAFSFGGGGKPFCE TIPGNPPATGPPSAPSTSVTTTSLEDSAPTSSKPATPETPVSTASSKLETPPSKLGELLFPSSLAGETLG SFSGLRVGQAEESTKPVSKASSTNLAGAQPAKPSGFSFPAASVLGKPVEPSVTSSAPAAPVSALSASTSS SSATVFGSVPLTSAAPSGLMSFSGASLASSKASFSFGNQQTSSAAAAAATPASTSVAPSLPASFPTLSFG GLLSSPSASSLPVSSGKITEEATPPAVPDKSDHSEVSASTPSLPLQPPSAQLPQASLQTSDPVKKEPVPA QTTDSRPSSSASLVASTESTPVAPGGPDSKTEAVSPASTFAGPGPAVVAAVLLGAGSAASEASGTPSTSS TVSSSTPTPATETAVFGTATSGSSVFTQPPAASSSSAFNQLSSNTATAPSATPVFGQVAASIASTAAATP QASSSGFGSPAFGASAPGVFGQTAFGQSPAFGQATSSPASGFSFSQPGFSSVPAFGQSVSSTPTSTSANV FGATSSTSSPGSFSFGQASTNTGGTLFGQNNPPAFGQSPGFGQGGSVFGSTSATTSTAAPSGFSFCQASG FGSSNTGSVFGQAASSGGSVFGQSSTSSGSVFGSGNATRGGGFFSGLGGKPSQDAANKNPFSSASGGFGS TPTPNTSNLFGNSGAKTFGGFGSSFGEQKPAGTFSSGGGSVASQGFGFSTPNKTGGFGAAPVFGSPPTFG GSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTASFGTLASQNAPTFGSLSQQT SGFGTPSSGFSGFGSGTGAFTFGSSNSSVQGFGGWRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001168559
ORF Size	6201 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001168559.1, NP_001162031.1
RefSeq Size	6553 bp
RefSeq ORF	6204 bp
Locus ID	296634
MW	211.4 kDa

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