Nup214 (NM_001168559) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1304977
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217734 representing NM_001168559 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACGAGATGGATGCCATGATCCCTGAGCGGGAGATGAAGGACTTTCAGTTTAGAGCTCTGAAGA AAGTGAGAATCTTTGACTCTCCTGAGGAGTTGCCCAAGGAGCGCTCGAGTGTGCTCACCGTATCCAATAA GTATGGCATGCTCTTTGCTGGTGGCACCAATGGCTTGAATGTCTTTCCTACTAAAAGCCTTCTTATCCAA AACAAACCTGGAGATGATCCCAATAAAATTGTTGACACAATCCAAGGCTTGAATGTCCCTATGAAATTCC CAGTTCATCACCTGGCACTGAGCTGTGATAACCTTACACTCTCTGCGTGCATGATGTCCAGTGAATATGG CTCCATTATAGCCTTTTTTGATGTTCGCACATTTTCAAATCAGGCTAAACCACTGAAACGCCCATTTACC TATCACAAGCTTTCAAACGGTGCCAGCGGGATGGTGAATGATATGAAGTGGAACCCCACTGTCCCTTCCA TGGCAGCAGTTTGTCTGGCTGATGGCAGCATTTCTGTCTTGCAAGTTACGGATGTGGTGAAAGTGTGCGC CACACTTCCTCCTTCATCGGGAGTCACGTGTGTATGCTGGAGCCCCAAAGGAAAGCAGCTGGCCGTGGGA AAGCAGAATGGAACTGTGGTCCAGTATCTTCCTACATTGCAGGAAAAAAAGGTCATTCCATGTCCTCCGT TTTATGAGGCAGACCACCCTGTCAGAGTTCTGGATGTGCTGTGGATTGGTACATACGTCTTCACGATAGT CTATGCTGGTGCGGATGGCGCACTGGAGACCTGTCCAGATGTGGTGATGGCTCTGCTTCCGAAAAAAGAA GAAAAGCACCCAGAAATATTTGTGAACTTCATGGAGCCCTGCTACAGCAGCTGCACAGAGAGACAACATC ATTACTACCTCAGCTACATCGAGGAGTGGGATTTAGTGCTGGCAGCATCTGCAGCGTCAACAGAAGTTAG CATCCTTGCTCGACAAAGTGATCAGACTAATTGGGAATCTTGGGTACTGGAAGACTCTAGTCGAGCTGAA CTGCCTGTGACAGACAAGAGCGATGACTCCTTGCCCGTGGGTGTTGCCATAGATTACACCAATGAAGTAG AAGTTGCCATCAATGAAGAAAAGACTCTTCCTCCTGCTCCGGTTCTCCTGTTACTTTCAACAGATGGTGT ACTTTGTCCATTTTATATGATCAATCAAAATCCCGGGGTTAGGTCCCTAATCAAGACCTTAGAGCTGATC TCAGCAGAGGGAGAGCGCCAGCCTAAGTCCTCAGGAAGTTTTCCTAGTACACCACCTTCCCCTCAAGCTC CACAGAACCTTGACGCTCCAGCAACAGCCTCTTCGCCTCTTCCACCGGTGTCTGCTGCTCCCACCGCCAC CTTCCCTTTGCCGTCTGCTGGAGGAGGCCCTTCTGTGTTCTCCTTTGGCCCTTCACCCTTCAAGTCCTCC TCCATCCCCGGGGAGCCCCCGCCATACCCCACTGGCTCTGACAGTTCCAGAGCAGCACCAGCCTCTGGCA CCCCCACCTTCTCTTTTGCGCCTCCTTCCAAAGGCACCTTGGCCCCGACCCCTGCAGTGGCTCCTGTGCC CACATCTGCTGCTCCCTTCACTTTCGGATCATCAGGTTTTAAGCCCCCCTTGGAAAGCACACCTATGTCC AGTGCCCCAAACACAGGGATGAAGCCTTCATTCCCACCCTCAACTTCTTCGGTGAAAGTCAACCTGAACG AGAAGTTCACCGCTGTGGCCTCCTCTGCACCCGTTCATAGCTCCACAAGCATACCCTCCGTGTTGCCGTT CTCTTCCTCCCCCAAGCCACCAGCTTCTGGACCACTCAGCCATCCCACGCCTCTCCCAGCATCACCCAGC TCCATGCCTTTGAAACCTTCAGTCTCTCCCTCTCCGTCAGTTGGCCGCTCCGCCCAGACTGCCCCGAGTT CAGCACCCTCCACAGGGCAGAAATCTCCTAGGGTAAATCCTCCAGTGCCAAAGTCAGGGTCCTCTCAGGC CAGAGCACTTCAGCCTCCTGTTACAGAGAAGCAGAGGCATCAGTGGAAAGAGTCAGACCCTGTGATGGCT GGAATTGGAGAGGAGATTGCACACTTCCAGAAAGAGCTGGAGGAGTTAAAGGCGCGAACCTCCAAAGCCT GTTTCCAGGTCGGCACCCCTGAGGAGATGAAGATGCTGCGCACAGAGTCAGACGACCTGCACACCTTCCT CTTTGAGATCAGAGAGGCCACAGAGTCTCTTCATGGAGACATAAGTACACTGAAAACAACTCTGCTGGAG GGCTTTGCTGGTGTTGAAGAAGCCAGAGAACAGCATGGAAGAAATCATGACTCTGGGTATCTCCATCTGC TTTATAAAAGACCACTGGATCCCAAGAGCGAGGCCCAGCTCCAGGAAATTCGCCGCCTTCACCAGTATGT GAAATTTGCTGTCCAGGATGTGAATGATGTCCTAGACTTAGAGTGGGATCGGCATCTGGAGCAGAAGAAA AGACAGAGGCGCCTAATTGTGCCAGAGAGAGAGACACTTTTCAACACACTAGCCAACAACCGGGAAATCA TCAACCAGCAGAGGAAGAGGTTGAATCACCTGGTGGACAGTCTGCAGCAGCTCCGTCTCTATAACCAGAC GTCCCCATGGAACCGGCCCTCAACGCTTTCAACGCAAAGTGACACTCCTAGTTTTGACAGTGACCTTGAA CACCTGCTAAAGACTACCATTGAATCTCACACCAAGCCTTCTCCTAGAGTGCCAGGTAAACTGTCCCCTG CGAAACAGGCCCAACTGAGGAACTTCTTGGCCAAAAGGAAGACCCCACCAGTGAGGTCTACTGCTCCAGC CAGCCTGTCTCGGTCAGCCTTCCTGTCTCAGAGGTACTATGAAGACTTAGATGAAGGCAGCTCCGCGTCG TCCGTAGCTCACCCTCTGGAGGGAGAAGATGCCCGGCCGACGCCGACCTGCAGAGAGGAGGAGGCTGTGG TCCCAGTCCCTCGGCATGCCCCCGTGGTCCGCACACCTTCTGTCCAACCCAGTCTCCTGCCTCAGGCGAT GCCTTTTGCAAAGCCTCACCTGATTCATAGTTCTTCACCTGCTGCGATGAGCTCTTCAATCTCTACGTCA GCTACCAAAGTTATTCCTCAAGGAGCCGACACCACAATGCTGGCAACTAAAACGGTAAAGCATGGTGCAC CAGGTCCTTCCCACACTGTCGCAGCTCCCCAGGCAGCGGCTGCTGCAGCCCTGAGACGGCAGATGGCCAG TCAGGCGCCAGCTGTGAGCACTCTGACCGAATCCACTTTGAAGAATGTCCCTCAAGTGGTGAATGTACAG GAACTGAAGAGTAACCCCTCACCCCCTTCTGCGGCCATGGGTTCTTCTGGGCCGCACTCAGCAGCCAAAG CCCCCCATGCAGTGTTAACCCCAGTGGCTGCCAGCCAAGCCAAGCAGGGGTCTCTGATAAATTCCTTCAA GCCGTCAGGACCCACACCAGTGTCCTGTCAGCTGTCCTCTGGGGATAAAGCTGTAGGTCCAGGGACGGCC AAGACAGAGTCACCTGTGACCTCCACCCCATCTACGGGGCAGCTCAACAAGCCTTTCTCATTCGCCTCCC CAGGGACCTTTACTTTTGGGACAATCACACCAGCCCCATCTTCTAGTTTCACTGCCACACCAGGGGCAGC ACCCCCCACTAAAGAGTCAAACCAGCTTGAGGCGTTCTCATTTGGCGGGGGAGGCAAACCTTTTTGTGAG ACCATTCCTGGAAACCCCCCTGCCACGGGACCCCCATCAGCACCCAGCACTTCTGTAACAACTACGTCTC TGGAAGACTCTGCTCCAACCAGCAGCAAACCTGCAACTCCTGAAACTCCTGTGTCCACTGCCTCTAGCAA ATTGGAAACTCCACCATCCAAGTTAGGAGAGCTTCTCTTCCCAAGTTCTCTAGCTGGAGAGACGCTAGGG AGCTTCTCAGGACTGCGTGTTGGCCAGGCTGAGGAGTCTACAAAGCCCGTTAGTAAGGCCTCCTCCACAA ACCTGGCCGGAGCCCAGCCAGCCAAGCCCTCAGGGTTTAGCTTTCCTGCTGCTTCCGTGTTAGGGAAGCC AGTGGAGCCCAGTGTGACGTCCTCTGCCCCAGCGGCACCAGTCTCAGCTCTGAGTGCGAGCACTAGCAGT TCCTCGGCAACTGTCTTTGGCAGTGTGCCCCTTACCAGTGCTGCACCTTCCGGGCTCATGAGTTTTAGTG GGGCCTCTTTGGCTAGTAGCAAAGCTAGTTTCTCATTTGGAAACCAACAGACTAGTAGTGCAGCAGCAGC AGCAGCTACCCCAGCATCCACCTCAGTTGCCCCTTCCCTTCCAGCATCTTTCCCTACTTTGTCATTTGGT GGCCTCCTGAGTTCACCATCAGCTTCTTCCCTGCCTGTGTCTTCTGGTAAGATCACAGAGGAGGCCACAC CACCAGCTGTGCCTGACAAGTCAGACCACAGTGAAGTGTCAGCATCAACACCCTCACTTCCACTACAGCC ACCGTCAGCCCAGCTTCCCCAGGCATCTCTGCAAACTTCTGACCCTGTCAAAAAAGAACCTGTTCCTGCG CAGACCACAGACAGCAGACCATCATCTTCTGCCAGCCTTGTGGCATCCACAGAATCCACTCCAGTCGCCC CAGGGGGCCCTGATAGCAAGACAGAAGCAGTGTCCCCTGCTTCCACCTTTGCAGGGCCTGGGCCGGCCGT GGTTGCAGCTGTTCTCCTAGGAGCAGGCTCGGCAGCCAGTGAAGCTTCCGGTACCCCTAGTACCTCCAGC ACTGTCTCCAGCAGTACCCCAACTCCTGCCACAGAGACAGCTGTATTTGGGACTGCCACTTCTGGCTCCT CAGTCTTTACACAGCCGCCTGCTGCCAGTTCGAGCTCAGCATTCAACCAGCTCTCCAGTAACACGGCCAC TGCACCCTCCGCCACCCCCGTGTTTGGGCAAGTGGCAGCCAGCATAGCCAGCACAGCCGCTGCCACGCCA CAGGCCAGCAGTTCAGGATTTGGCAGCCCTGCGTTTGGTGCTTCGGCTCCTGGAGTGTTCGGACAGACAG CATTTGGGCAGTCCCCAGCCTTTGGGCAAGCAACAAGCAGCCCTGCGAGTGGCTTCTCCTTCAGCCAACC TGGGTTCAGCTCTGTCCCTGCTTTTGGCCAGTCTGTCTCCTCCACCCCCACATCCACCAGTGCCAATGTC TTCGGAGCCACCTCAAGTACCAGCAGCCCTGGTTCCTTCTCATTTGGACAGGCTTCTACCAATACAGGAG GGACGCTATTTGGCCAAAACAACCCTCCTGCTTTTGGTCAGAGCCCGGGCTTTGGGCAGGGAGGCTCTGT GTTTGGTAGCACCTCGGCCACTACCTCTACAGCAGCACCTTCAGGGTTTAGCTTTTGTCAAGCCTCCGGT TTTGGGTCTAGTAATACTGGTTCTGTGTTTGGTCAGGCAGCCAGTAGCGGTGGGTCAGTCTTTGGCCAGT CATCCACTTCCAGTGGCAGCGTGTTTGGGTCTGGAAATGCTACAAGAGGGGGCGGTTTCTTCAGTGGCCT TGGAGGAAAACCCAGCCAGGATGCTGCCAACAAAAACCCATTCAGCTCGGCCAGTGGGGGCTTTGGGTCT ACGCCTACACCCAATACCTCTAACCTGTTCGGGAACAGCGGAGCTAAGACCTTCGGAGGGTTCGGCTCCT CCTTCGGGGAGCAGAAGCCTGCTGGCACATTCAGCTCTGGTGGTGGGAGCGTGGCATCCCAAGGTTTTGG ATTTTCCACTCCAAATAAAACAGGTGGCTTCGGTGCTGCTCCAGTGTTTGGCAGCCCTCCTACTTTTGGG GGATCCCCTGGGTTTGGAGGGGTGCCAGCATTCGGTTCAGCCCCAGCCTTTACAAGCCCTCTGGGCTCGA CGGGAGGCAAAGTGTTCGGAGAGGGTACTGCGGCCGCCAGCGCAGGAGGATTCGGGTTCGGGAGTAGCAG CAACACTGCATCCTTCGGTACCCTCGCCAGTCAGAACGCACCCACTTTCGGATCCTTGTCCCAGCAGACC TCTGGCTTCGGGACCCCGAGCAGTGGATTCTCTGGGTTTGGATCAGGCACAGGAGCATTCACCTTCGGAT CATCTAACTCGTCTGTCCAGGGCTTCGGTGGCTGGAGAAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001168559
Insert Size	6204 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001168559.1, NP_001162031.1
RefSeq Size	6553 bp
RefSeq ORF	6204 bp
Locus ID	296634

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