Gemin5 (NM_001172089) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217646 representing NM_001172089 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACCAGAGCCGCGGACGCTTCCGCCGTCCCCCAACTGGTACTGCGCGCGCTGCAGCGACGCGGCGC CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGCCCGGGCGCCGGCGC GAGCCCAGGGGCGCCCCCGTTTCGAGTTGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGATGATGGGACTGTGAAGATTT GGGATGTAGAGACCAAGGCTGTAGTGACAGAACACGCGCTCCATCAGCATACAATATCAGCACTGCATTG GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTTGTTTTCTGTTACTGGCTTAAC AGAAGTGACAGCCAGCACCTCTTCACAGAGCCCAGGGCCATATTCTGTCTGACCTGTTCACCTCATCACG AAAATCTAGTAGCCATTGGCTACAAAGACGGCATAGTGATTATCATTGACATCAGTAAAAAGGGAGAAGT TGTTCACAGGCTTCGAGGGCACGACGATGAAATCCACTCCATAGCCTGGTGTCCTTTGTCTGGTGAAGAC TGCTTATCTATAAACCAAGAGGAAAACTCAGAAGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAA CCCCCATCACAAAAGGCTGCTACTTAGCTACAGGAAGCAAGGATCAGACCATTCGAATCTGGAGCTGTTC CCGAGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTTCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACA GTTAAAGAACGTCTTTGGTTGACACTTCATTGGCCCAAGAACCAACCAACACAGCTGGTATCCAGTTGTT TTGGAGGTGAGCTGCTGCTGTGGGACCTCACCCAGTCATGGAGACGGAAGTACACGCTCTTCAGCACCTC AGCGGAAGGGCACAATCACTCCAGAATTGTCTTCAACTTGTGTCCTTTGAAGACTGAGGATGGCAAACAG CTGCTATTGTCCACGTCCATGGACAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGA CATTACCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCACCTGTGGACGTGGGCTCCCTGGCCAT AGGTGTTGGGGACGGCATGATCCGGGTATGGAATACACTCTCCATAAAGAACAACTACGATGTGAAAAAC TTCTGGCAAGGAGTCAAGTCCAAGGTCACGGCGCTATGCTGGCATCCAAACAAGGAGGGCTGCTTAGCCT TTGGGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCAC ATATCATAAGAAGACCGTATATAGGTTGGCTTGGGGGCCACCAGTGCCCCCCATGTCACTTGGAGGGGAA GGAGACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCATTGTGCTGCAGCATAATCCCTGGA AGCTCAGTGGAGAGGCCTTTGACATCAACAAACTCATGAGAGACACCAACTCCATTAGATACAAGCTGCC TGTGCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCATGGCTCTTGGAAATGAAGATGGATCAATA GAAATATTCCAAGTCCCAAACTTGAAACTGCTCTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCA TAGTCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGCCTCCTTGCTTCTGGCTCCAACAATGC AGTTATCTACGTGCACAACCTGAAAGCTGTCCTAGAGAGCAATCCTGAGTCTCCAATAACCATCACAGAG CCCTACCGGACCCTCTCAGGGCACACAGCCAAGGTTACCAGTCTGGCATGGAGCCCACATCATGATGGAA GGCTGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGAGAAGAACCTTTGTTCAA TTTCCGAGGACATCGAGGCCGGCTGTTATGTGTTGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCA GGTGCAGATGACTTCTGTGTCTACAGGTGGCTGACCTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGA AAAAATGTATTGAATTAGAGAAAAAACGGCTCTCTCAATTTAAGCCAAAGCTAAAAAAGAAGAAAAAACC TACCTTGCGACTGCCCATAAAGCAAGATTCATCCATTGGCAATGAAGATGAGAGTGTGAGGGAGAACTTG GCACCTGTTGAGAATGGCTTGTCCGATCAGGATGGTGAGGAGGAAGCCCAGGAGCCGGAGCTGCCTCCCT CTCCCGTGGTTTGTGGAGAACCTGTTTCCTGCACTGCTGTCTCCTTAGGCTTGGAAAAGTCAAAAGTCAC TGTTACTAGTAAAGTCGCCTCACTGAAAAAGGAGCCACCTAAAGAGAAGCCAGAAGCCTTGCTCAAGAAG AGGAAGGCTCGCTCCATGCTCCCCCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAG ACTGTTTGGTTCTAGCAACGGCAACACACGCCAGAGCAGAACTGCATGAAGATGTGTCTGCCGACCTTGA GGAACGATTTCACTTGGGCCTTTTCACAGACAGGGCTGCTCTGTACAGGATGATGGAGACAGAGGGGAAA GGTCACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAGGGAGATCTGAAAGGCG TTCTCCAGGCTGCAGCAGAGAGAGGCGAGCTCACAGACAGTCTCGTGGCCGTGGCACCAGTAGCTGGCTA CAGTGTGTGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGATCAGTATGTCAAGGCT GCCTCCTACCTGCTATCCATCCACAGAGTGTACGAAGCTGTGGAGCTTCTCAGGTCAAACCATCTCTACA GAGAAGCTATTGCAGTTGCCAAGGCTCGGCTGCGCCCTGAGGACCCTGTTCTGAAGGACCTGTACCTCAG CTGGGGGTCCATCCTGGAGAGAGATGGCCACTATGCAATTGCAGCCAAGTGCTACTTAGGAGCCACTTCC GCCTATGATGCAGCCAAAGTCCTAGCCAGAAAGGGAGATGCAGCCTCTCTTAGAACAGCTGCAGAGCTGG CTGCCATAGCAGGAGAGAGTGAGCTGGCTGCTTCCCTGGCTCTTAGGTGCGCCCAAGAGCTGCTTCTGCT GAAGAACTGGGTGGGTGCACAGGAAGCCTTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTGTTC TGCCTCCTTGAGCTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCCAGAGGTCAGAGGCCCCTCTTCCA TTTACCACCAGTGGGCCACTGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACTGGTGTGTGGCGCAGCGC CTTCAGTGTGGACACCCCGGAGCAGTGTCGGGCCGCCTTGCAGAAGCTACAGGATGTCAAGTACCCATCA GCAACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTAACCTTAGCAATGCTGA GCCAGCAGGCTGCTTCCTGGGAGGAGGCTGTCCCTGCACTGCTGCAGGCTGTGGTTCGGAGCTACACCTC AGGGAATTTCACCCTCATGCAAGAAATCTACTCAGCCTTTCTCCCAGGCGGCTGTGACCACCTACGAGAC AAACTAGGTGACCTATCTCCTACCATGGCGGCTTTCAAAAGCTTAGAGGCCTTTTTCATTTATGGGCAAC TGTATGAAGTCTGGTGGTCACTCTGTGGGCCTGGCTCAGAATCAAGCATCTGGGTGTGGTCGGCTGAAAG CACAGTCTCTGATAAACAGAGCAAGCCAGGGGACAGTGCCAGTGCTGAAGACATGGAGCAGCCTCTAGGC CCAGGCCTGAGGCTCAGTGCGGAGAGTGAACGTTTGCTGAGTGCCTGCAAGGAGCTCTTCTCAGAGAGGC ATGCCAGCCTACAGACCTCCCAGAGGACTGTTGCTGAAGTCCAAGAGACGCTGGCAGAGATGATCCGCCA GCACCAAAAGAGTCAGCTCTGCAAGGCCACAGCCAACGGCCCCAACAGAGATGAACCCAGCCAGCAAGCA GAGCGGGCCCCTTCCCAACCTCCGAGCCCAACAGAAGAAAGAAATGCACCGGTGTCTCTCCCTGAATTAA CCAGAAGGCTCACAGAGGCAAACGAGAGAATAGCTGAGTTTCCAGAGAGTGTAAAGGCCTGGCCCTTTCC AGATGTGCTGGAGTGCTGTCTCGTCTTACTTCACATCGGGTCCCAGTGTCCTGGTGCTGTGAACCCTGAG ATGCAGCAACAGGCCCAGAAGCTCCTTCAGAAGTATGGCCACACTAGAGCTTACAGAAGACACTGCCAGA TTCGACACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217646 representing NM_001172089 Red=Cloning site Green=Tags(s) MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLN RSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGED CLSINQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPT VKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQ LLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKN FWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGE GDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSI EIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITE PYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYS GADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENL APVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKK RKARSMLPLSTSLDHRSKEELHRDCLVLATATHARAELHEDVSADLEERFHLGLFTDRAALYRMMETEGK GHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKA ASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATS AYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVF CLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPS ATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRD KLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLG PGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQA ERAPSQPPSPTEERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPE MQQQAQKLLQKYGHTRAYRRHCQIRHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001172089
ORF Size	4491 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001172089.1, NP_001165560.1
RefSeq Size	5012 bp
RefSeq ORF	4494 bp
Locus ID	691231
MW	166.1 kDa

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