Gemin5 (NM_001172089) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217646 representing NM_001172089 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACCAGAGCCGCGGACGCTTCCGCCGTCCCCCAACTGGTACTGCGCGCGCTGCAGCGACGCGGCGC CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGCCCGGGCGCCGGCGC GAGCCCAGGGGCGCCCCCGTTTCGAGTTGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGATGATGGGACTGTGAAGATTT GGGATGTAGAGACCAAGGCTGTAGTGACAGAACACGCGCTCCATCAGCATACAATATCAGCACTGCATTG GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTTGTTTTCTGTTACTGGCTTAAC AGAAGTGACAGCCAGCACCTCTTCACAGAGCCCAGGGCCATATTCTGTCTGACCTGTTCACCTCATCACG AAAATCTAGTAGCCATTGGCTACAAAGACGGCATAGTGATTATCATTGACATCAGTAAAAAGGGAGAAGT TGTTCACAGGCTTCGAGGGCACGACGATGAAATCCACTCCATAGCCTGGTGTCCTTTGTCTGGTGAAGAC TGCTTATCTATAAACCAAGAGGAAAACTCAGAAGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAA CCCCCATCACAAAAGGCTGCTACTTAGCTACAGGAAGCAAGGATCAGACCATTCGAATCTGGAGCTGTTC CCGAGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTTCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACA GTTAAAGAACGTCTTTGGTTGACACTTCATTGGCCCAAGAACCAACCAACACAGCTGGTATCCAGTTGTT TTGGAGGTGAGCTGCTGCTGTGGGACCTCACCCAGTCATGGAGACGGAAGTACACGCTCTTCAGCACCTC AGCGGAAGGGCACAATCACTCCAGAATTGTCTTCAACTTGTGTCCTTTGAAGACTGAGGATGGCAAACAG CTGCTATTGTCCACGTCCATGGACAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGA CATTACCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCACCTGTGGACGTGGGCTCCCTGGCCAT AGGTGTTGGGGACGGCATGATCCGGGTATGGAATACACTCTCCATAAAGAACAACTACGATGTGAAAAAC TTCTGGCAAGGAGTCAAGTCCAAGGTCACGGCGCTATGCTGGCATCCAAACAAGGAGGGCTGCTTAGCCT TTGGGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCAC ATATCATAAGAAGACCGTATATAGGTTGGCTTGGGGGCCACCAGTGCCCCCCATGTCACTTGGAGGGGAA GGAGACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCATTGTGCTGCAGCATAATCCCTGGA AGCTCAGTGGAGAGGCCTTTGACATCAACAAACTCATGAGAGACACCAACTCCATTAGATACAAGCTGCC TGTGCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCATGGCTCTTGGAAATGAAGATGGATCAATA GAAATATTCCAAGTCCCAAACTTGAAACTGCTCTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCA TAGTCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGCCTCCTTGCTTCTGGCTCCAACAATGC AGTTATCTACGTGCACAACCTGAAAGCTGTCCTAGAGAGCAATCCTGAGTCTCCAATAACCATCACAGAG CCCTACCGGACCCTCTCAGGGCACACAGCCAAGGTTACCAGTCTGGCATGGAGCCCACATCATGATGGAA GGCTGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGAGAAGAACCTTTGTTCAA TTTCCGAGGACATCGAGGCCGGCTGTTATGTGTTGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCA GGTGCAGATGACTTCTGTGTCTACAGGTGGCTGACCTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGA AAAAATGTATTGAATTAGAGAAAAAACGGCTCTCTCAATTTAAGCCAAAGCTAAAAAAGAAGAAAAAACC TACCTTGCGACTGCCCATAAAGCAAGATTCATCCATTGGCAATGAAGATGAGAGTGTGAGGGAGAACTTG GCACCTGTTGAGAATGGCTTGTCCGATCAGGATGGTGAGGAGGAAGCCCAGGAGCCGGAGCTGCCTCCCT CTCCCGTGGTTTGTGGAGAACCTGTTTCCTGCACTGCTGTCTCCTTAGGCTTGGAAAAGTCAAAAGTCAC TGTTACTAGTAAAGTCGCCTCACTGAAAAAGGAGCCACCTAAAGAGAAGCCAGAAGCCTTGCTCAAGAAG AGGAAGGCTCGCTCCATGCTCCCCCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAG ACTGTTTGGTTCTAGCAACGGCAACACACGCCAGAGCAGAACTGCATGAAGATGTGTCTGCCGACCTTGA GGAACGATTTCACTTGGGCCTTTTCACAGACAGGGCTGCTCTGTACAGGATGATGGAGACAGAGGGGAAA GGTCACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAGGGAGATCTGAAAGGCG TTCTCCAGGCTGCAGCAGAGAGAGGCGAGCTCACAGACAGTCTCGTGGCCGTGGCACCAGTAGCTGGCTA CAGTGTGTGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGATCAGTATGTCAAGGCT GCCTCCTACCTGCTATCCATCCACAGAGTGTACGAAGCTGTGGAGCTTCTCAGGTCAAACCATCTCTACA GAGAAGCTATTGCAGTTGCCAAGGCTCGGCTGCGCCCTGAGGACCCTGTTCTGAAGGACCTGTACCTCAG CTGGGGGTCCATCCTGGAGAGAGATGGCCACTATGCAATTGCAGCCAAGTGCTACTTAGGAGCCACTTCC GCCTATGATGCAGCCAAAGTCCTAGCCAGAAAGGGAGATGCAGCCTCTCTTAGAACAGCTGCAGAGCTGG CTGCCATAGCAGGAGAGAGTGAGCTGGCTGCTTCCCTGGCTCTTAGGTGCGCCCAAGAGCTGCTTCTGCT GAAGAACTGGGTGGGTGCACAGGAAGCCTTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTGTTC TGCCTCCTTGAGCTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCCAGAGGTCAGAGGCCCCTCTTCCA TTTACCACCAGTGGGCCACTGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACTGGTGTGTGGCGCAGCGC CTTCAGTGTGGACACCCCGGAGCAGTGTCGGGCCGCCTTGCAGAAGCTACAGGATGTCAAGTACCCATCA GCAACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTAACCTTAGCAATGCTGA GCCAGCAGGCTGCTTCCTGGGAGGAGGCTGTCCCTGCACTGCTGCAGGCTGTGGTTCGGAGCTACACCTC AGGGAATTTCACCCTCATGCAAGAAATCTACTCAGCCTTTCTCCCAGGCGGCTGTGACCACCTACGAGAC AAACTAGGTGACCTATCTCCTACCATGGCGGCTTTCAAAAGCTTAGAGGCCTTTTTCATTTATGGGCAAC TGTATGAAGTCTGGTGGTCACTCTGTGGGCCTGGCTCAGAATCAAGCATCTGGGTGTGGTCGGCTGAAAG CACAGTCTCTGATAAACAGAGCAAGCCAGGGGACAGTGCCAGTGCTGAAGACATGGAGCAGCCTCTAGGC CCAGGCCTGAGGCTCAGTGCGGAGAGTGAACGTTTGCTGAGTGCCTGCAAGGAGCTCTTCTCAGAGAGGC ATGCCAGCCTACAGACCTCCCAGAGGACTGTTGCTGAAGTCCAAGAGACGCTGGCAGAGATGATCCGCCA GCACCAAAAGAGTCAGCTCTGCAAGGCCACAGCCAACGGCCCCAACAGAGATGAACCCAGCCAGCAAGCA GAGCGGGCCCCTTCCCAACCTCCGAGCCCAACAGAAGAAAGAAATGCACCGGTGTCTCTCCCTGAATTAA CCAGAAGGCTCACAGAGGCAAACGAGAGAATAGCTGAGTTTCCAGAGAGTGTAAAGGCCTGGCCCTTTCC AGATGTGCTGGAGTGCTGTCTCGTCTTACTTCACATCGGGTCCCAGTGTCCTGGTGCTGTGAACCCTGAG ATGCAGCAACAGGCCCAGAAGCTCCTTCAGAAGTATGGCCACACTAGAGCTTACAGAAGACACTGCCAGA TTCGACACACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001172089
Insert Size	4494 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001172089.1, NP_001165560.1
RefSeq Size	5012 bp
RefSeq ORF	4494 bp
Locus ID	691231

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