Myom2 (NM_001169141) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217634 representing NM_001169141 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGGTTGTAGTGCCTTTCTACCAGAAAAGACACAAGCACTTTGACCAGTCATATCGAAACATCC AAACCAGGTACCTCCTGGACCGATATGCATCGAAAAAGCAAGCGTCCAGTCAATCATCATCCCAGAGGTC TCTTACGGAGAGGTCCTCTTCGAAGAGAGCTTCCAGTCAGAGCTCCGCTGAGGGCATGACCTGTAGGCTC TGTGCAAAGCGGATGAGCGCATCAGAAGAGGAGGAAGTTGAAAATGAAAACAGGTACCGGTCTCTAGCGG CTTCTTACGGAGAAGCCAAAAGGCAGCGTTTCCTCAGTGAGCTGGCCCAGCTGGAGGAGAACGTACAATT GGCACGGACACAGGCTCGGAACAAGCTGGACAAGTACTTCCTGGAGCAGACGGTGGCAGACAATCTGGCT TTGGAGAGAAGCTCATTTGAAGACCGGCTGAGCAGGGCCCCTGAGATCCTGGTGAGGTTAAGGTCCCACA CCATCTGGGAGCGGATGTCTGTGAGGCTCTGTTTCACTGTACAAGGATTCCCCACTCCTGTGGTGCAATG GTACAAAGACGGCAGTCTGATCTGCCAGGCAGGGGAGCCAGGCAAGTACCGGATTGAGAGCAGGTACGGA GTGCACACTCTGGAGATCAACAGGGCCGACTTTGAGGACACAGCGACCTATTCAGCAGTAGCAACGAATC CCCATGGACAAGTGTCTACCAATGCTGCGGTGGTGGTGAGAAGGTACCGAGGAGATGAGGAGCCCTACCA CTCAGTAGGACTCCCAATTGGATTGCCTCTCTCATCTGTGATCCCGTACACACATTTTGATGTCCAGTTT TTGGAGAAATTCGGGGTCACCTTCAGAAGAGAGGGCGAGACGGTCACACTCAAGTGCACATTATTGGTGA CACCAGATCTGAAGAGGGTACAGCCTCGAGCCGAGTGGTACCGGGATGATGTGCTGCTGAAAGAGTCCAA GTGGACCAAGATGTTCTTCGGAGAAGGCCAGGCCTCACTGTCCTTCAGCCACCTGAACAAGGATGATGAG GGGCTTTACACCTTGAGGATCGTGTCCCGGGGTGGAGTCAGTGACCACAGCGCTTTCATGTTTGTCAGAG ATGCTGACCCGCTGGTCACAGGGGCTCCTGGGGCACCCATGGACCTGCAGTGCCATGATGCCAACCGAGA CTATGTTATCGTGACTTGGAAACCACCCAACACCACTACTGAGAGCCCCGTCATTGGCTACTTCATTGAC AAATGTGAGGTGGGAACTAACAACTGGGTTCAGTGCAATGACGCACCGGTAAAGATCTGCAAGTACCCCG TCACCGGACTTTTCGAAGGAAGATCGTACGTGTTCCGCGTGAGGGCTGTCAATAACGCCGGCATAAGCAG GCCTTCCAGGATCTCCGATGCTGTGGCTGCCCTTGACCCTGTGGACCTCAGAAGGTTGCAAGCGATTCAT TTGGAAGGAGAGAAAGAAATCGTCATCTACCAGGATGACCTTGAAGGTGATGTTCAGATTCCAGGACCTC CCACCAATGTCCAGGCTTCAGAGGTCAGCAGAAACTACGTTGTCCTCAGCTGGGATCCTCCTTCTCCCCG TGGCAAGGAGCCCTTAATGTACTTCATTGAGAAGTCGGCAGTTGGGAGTGGCAGCTGGCAGAGGGTCAAT GCCCAGACAGCAGTGAGATCTCCTCGGTATGCTGTCTTTGACTTGGCGGAAGGGAAGTCCTATGTGTTCC GGGTTTTGTCAGCCAATAAGCATGGCCTGAGTGACCCATCAGAGATAACACCACCTATTCAGGCTCAGGA CATGATTGTGGTTCCCTCTGCACCTGGCCGGGTGCTTGCTTCCAGGAATACAAAGACATCTGTCGTGGTC CAGTGGGACAGACCAAAGCATGAAGAAGACCTGCTGGGCTACTATGTGGACTGCTGTGTGGCGGGAACAA ACATGTGGGAACCATGTAACCATAAACCCATTGGATATAACAGGTTCGTGGTACACGGCTTAACCACCGG TGAGCAGTACATCTTCCGGGTTAAGGCTGTGAATGCCGTGGGGACCAGCGAGAACTCCCAGGAGTCAGAG GTCATCAAGGTCCAGGCAGCCCTCACTGTCCCCTCTCATCCCTACGGGATTACCCTTCTCAACTGTGACG GTCACTCCATGACCCTGGGCTGGAAAGTGCCCAAGTTCAGCGGCGGCTCTGCCATCATAGGTTACTACCT GGACAAGCGGGAAGTGCATCATAAGAACTGGCACGAGATCAACTCTTCGCCTGTCAAAGAGAGGATCTTA ACGGTGGAAGGTTTGACTGAAGGCTCCTTGTATGAGTTCAAAATCGCTGCTACCAACCTGGCTGGGATCG GGCAGCCATCGGATCCCAGTGAGCACTTCAAGTGTGAGGCCTGGACAGCCCCAGAACCTGGTCCCGCCTA TGACTTGACGTTCTGTGAGGTCCGAGACACATCTCTGGTCATACTGTGGAAAGCCCCGGTGTACTCCGGC AGCAGTCCTGTTTCTGGATATTTTGTGGATTTTAAGGAAGAGGATTCTGGGGAGTGGAAGACCACCAGCG AGGCAGCAACTCCAAATCGCTACTTAAAGGTCTGTGACCTGCATCAAGGTAAGACCTATGTATTCAGAGT TCGAGCAGTGAATGCCAGTGGACCAGGGAAGCCGTCAGACACATCGGAGCCTGTGCTCGTGGAGGCCCGG CCAGGCACAAAGGAGATCAGTGCTGGTGTGGATGAGGAGGGCAACATCTACCTTGGGTTTGACTGCCAGG AAATGACAGACGCCTCGCAGTTCACCTGGTGCAAGGCCTATGAGGAGATCGCCGATGAGGAGCGGTTCGA GGTTCACACAGAGGGTGATCACTCAAAGCTGTACTTCAAGAATCCAGATAAAATAGACATAGGGACATAT TCTGTATCTGTAAGTGACACGGATGGTGTGTCTTCCAGCTTCGTTTTGGATGAGGAAGAACTTGAGCGTT TGATGGCGTTGAGCAATGAGATAAAGAATCCCACAATTCCTCTGAAATCAGAATTAGCATATGAGATTTT CGATAAGGGCCAGGTCCGATTCTGGCTCCAGGCAGAGCACCTCTCACCTGATGCCAACTTCCGGTTCATT ATTAATGACAGAGAAGTTTCTGACAGTGAGACACACAGAATTAAATGCGACAGATCGACAGGCATGATTG AGATGGTCATGGATCGGTTTACTATTGAAAACGAAGGGACCTACACTGTGCAGATTCAAGATGGAAAAGC CAAGAATCAGTCTTCGCTGGTGCTCATTGGAGATGCATTCAGGGCTGTCCTAGAAGAGGCTGAATTTCAA AGGAAGGAATTTCTACGGAAACAAGGCCCTCATTTTGCTGAGTATTTGCACTGGGATGTTACTGAAGAGT GTGAAGTTCGGCTTATCTGTAAGGTTGCAAACACCAAGAGGGAAACCGTTTTCAAATGGCTCAAGGATGA TGTCCTTTACGAAACCGAGACACCACCGCCGGACCTGGAGAAGGGGATCTGTGAGCTGCTTATACCCAAG TTGTCTAAGAAGGACCATGGCGAGTACAAAGCAACCTTGAAAGATGACAGAGGTCAAGATGTATCTGTCT TGGAAGTAGCTGGCAAAGTGTACGAGGACATGATCTTGGCAATGAGCCGAGTGTGTGGAGCCTCTGCCTC ACCCCTGAAGGTTCTCTGCACTCCAGAGGGCATCCGGCTTCAGTGCTTCATGAAGTATTTCACAGAGGAA ATGAAGGTCAGCTGGTACCACAAAGAGGCTAAGATCTCATCCAGTGAGCACATGCGGATCGGAGGAAGCG AGGAGATGGCCTGGCTGCAGATCTGTGAGCCCACAGAGAAGGACAAGGGGAAATACACTTTCGAGATCTT CGATGGCAAAGACAACCACCAACGCTCACTGGACCTCTCTGGGCAAGCCTTCGATGAAGCATATGCCGAG TTCCAGCAGCTCAAGGCTGCTGCATTTGCTGAGAAGAATCGTGGCAAGGTGATTGGCGGTTTGCCCGACG TGGTGACCATAATGGAAGGCAAGACCTTGAACCTCACCTGCACAGTATTTGGAAACCCGGACCCTGAGGT GGTCTGGTTTAAGAACGACAAGGACATTGAGCTAAGCGAGCACTTCTCGGTGAAGGTGGAGCAGTCCAAG TACGTCAGCCTGACCATTAAGGGGGTGACTGCTGAGGACTCGGGCAAGTACAGCATCAACGTGAAGAACA AATACGGAGGAGAGAAGATCGATGTCACCGTCAGCGTGTACAAACACGGGGAGAAGATCCCCGACATCTC AGCTCCCCAGCAGGCCAAACCCAAGCTTATCCCAGCTTCCACCTCAGCTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217634 representing NM_001169141 Red=Cloning site Green=Tags(s) MSLVVVPFYQKRHKHFDQSYRNIQTRYLLDRYASKKQASSQSSSQRSLTERSSSKRASSQSSAEGMTCRL CAKRMSASEEEEVENENRYRSLAASYGEAKRQRFLSELAQLEENVQLARTQARNKLDKYFLEQTVADNLA LERSSFEDRLSRAPEILVRLRSHTIWERMSVRLCFTVQGFPTPVVQWYKDGSLICQAGEPGKYRIESRYG VHTLEINRADFEDTATYSAVATNPHGQVSTNAAVVVRRYRGDEEPYHSVGLPIGLPLSSVIPYTHFDVQF LEKFGVTFRREGETVTLKCTLLVTPDLKRVQPRAEWYRDDVLLKESKWTKMFFGEGQASLSFSHLNKDDE GLYTLRIVSRGGVSDHSAFMFVRDADPLVTGAPGAPMDLQCHDANRDYVIVTWKPPNTTTESPVIGYFID KCEVGTNNWVQCNDAPVKICKYPVTGLFEGRSYVFRVRAVNNAGISRPSRISDAVAALDPVDLRRLQAIH LEGEKEIVIYQDDLEGDVQIPGPPTNVQASEVSRNYVVLSWDPPSPRGKEPLMYFIEKSAVGSGSWQRVN AQTAVRSPRYAVFDLAEGKSYVFRVLSANKHGLSDPSEITPPIQAQDMIVVPSAPGRVLASRNTKTSVVV QWDRPKHEEDLLGYYVDCCVAGTNMWEPCNHKPIGYNRFVVHGLTTGEQYIFRVKAVNAVGTSENSQESE VIKVQAALTVPSHPYGITLLNCDGHSMTLGWKVPKFSGGSAIIGYYLDKREVHHKNWHEINSSPVKERIL TVEGLTEGSLYEFKIAATNLAGIGQPSDPSEHFKCEAWTAPEPGPAYDLTFCEVRDTSLVILWKAPVYSG SSPVSGYFVDFKEEDSGEWKTTSEAATPNRYLKVCDLHQGKTYVFRVRAVNASGPGKPSDTSEPVLVEAR PGTKEISAGVDEEGNIYLGFDCQEMTDASQFTWCKAYEEIADEERFEVHTEGDHSKLYFKNPDKIDIGTY SVSVSDTDGVSSSFVLDEEELERLMALSNEIKNPTIPLKSELAYEIFDKGQVRFWLQAEHLSPDANFRFI INDREVSDSETHRIKCDRSTGMIEMVMDRFTIENEGTYTVQIQDGKAKNQSSLVLIGDAFRAVLEEAEFQ RKEFLRKQGPHFAEYLHWDVTEECEVRLICKVANTKRETVFKWLKDDVLYETETPPPDLEKGICELLIPK LSKKDHGEYKATLKDDRGQDVSVLEVAGKVYEDMILAMSRVCGASASPLKVLCTPEGIRLQCFMKYFTEE MKVSWYHKEAKISSSEHMRIGGSEEMAWLQICEPTEKDKGKYTFEIFDGKDNHQRSLDLSGQAFDEAYAE FQQLKAAAFAEKNRGKVIGGLPDVVTIMEGKTLNLTCTVFGNPDPEVVWFKNDKDIELSEHFSVKVEQSK YVSLTIKGVTAEDSGKYSINVKNKYGGEKIDVTVSVYKHGEKIPDISAPQQAKPKLIPASTSAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169141
ORF Size	4392 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169141.1, NP_001162612.1
RefSeq Size	4513 bp
RefSeq ORF	4395 bp
Locus ID	306616
MW	164.7 kDa
Gene Summary	a major M-line structural protein [RGD, Feb 2006]

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