Myom2 (NM_001169141) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217634 representing NM_001169141 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGGTTGTAGTGCCTTTCTACCAGAAAAGACACAAGCACTTTGACCAGTCATATCGAAACATCC AAACCAGGTACCTCCTGGACCGATATGCATCGAAAAAGCAAGCGTCCAGTCAATCATCATCCCAGAGGTC TCTTACGGAGAGGTCCTCTTCGAAGAGAGCTTCCAGTCAGAGCTCCGCTGAGGGCATGACCTGTAGGCTC TGTGCAAAGCGGATGAGCGCATCAGAAGAGGAGGAAGTTGAAAATGAAAACAGGTACCGGTCTCTAGCGG CTTCTTACGGAGAAGCCAAAAGGCAGCGTTTCCTCAGTGAGCTGGCCCAGCTGGAGGAGAACGTACAATT GGCACGGACACAGGCTCGGAACAAGCTGGACAAGTACTTCCTGGAGCAGACGGTGGCAGACAATCTGGCT TTGGAGAGAAGCTCATTTGAAGACCGGCTGAGCAGGGCCCCTGAGATCCTGGTGAGGTTAAGGTCCCACA CCATCTGGGAGCGGATGTCTGTGAGGCTCTGTTTCACTGTACAAGGATTCCCCACTCCTGTGGTGCAATG GTACAAAGACGGCAGTCTGATCTGCCAGGCAGGGGAGCCAGGCAAGTACCGGATTGAGAGCAGGTACGGA GTGCACACTCTGGAGATCAACAGGGCCGACTTTGAGGACACAGCGACCTATTCAGCAGTAGCAACGAATC CCCATGGACAAGTGTCTACCAATGCTGCGGTGGTGGTGAGAAGGTACCGAGGAGATGAGGAGCCCTACCA CTCAGTAGGACTCCCAATTGGATTGCCTCTCTCATCTGTGATCCCGTACACACATTTTGATGTCCAGTTT TTGGAGAAATTCGGGGTCACCTTCAGAAGAGAGGGCGAGACGGTCACACTCAAGTGCACATTATTGGTGA CACCAGATCTGAAGAGGGTACAGCCTCGAGCCGAGTGGTACCGGGATGATGTGCTGCTGAAAGAGTCCAA GTGGACCAAGATGTTCTTCGGAGAAGGCCAGGCCTCACTGTCCTTCAGCCACCTGAACAAGGATGATGAG GGGCTTTACACCTTGAGGATCGTGTCCCGGGGTGGAGTCAGTGACCACAGCGCTTTCATGTTTGTCAGAG ATGCTGACCCGCTGGTCACAGGGGCTCCTGGGGCACCCATGGACCTGCAGTGCCATGATGCCAACCGAGA CTATGTTATCGTGACTTGGAAACCACCCAACACCACTACTGAGAGCCCCGTCATTGGCTACTTCATTGAC AAATGTGAGGTGGGAACTAACAACTGGGTTCAGTGCAATGACGCACCGGTAAAGATCTGCAAGTACCCCG TCACCGGACTTTTCGAAGGAAGATCGTACGTGTTCCGCGTGAGGGCTGTCAATAACGCCGGCATAAGCAG GCCTTCCAGGATCTCCGATGCTGTGGCTGCCCTTGACCCTGTGGACCTCAGAAGGTTGCAAGCGATTCAT TTGGAAGGAGAGAAAGAAATCGTCATCTACCAGGATGACCTTGAAGGTGATGTTCAGATTCCAGGACCTC CCACCAATGTCCAGGCTTCAGAGGTCAGCAGAAACTACGTTGTCCTCAGCTGGGATCCTCCTTCTCCCCG TGGCAAGGAGCCCTTAATGTACTTCATTGAGAAGTCGGCAGTTGGGAGTGGCAGCTGGCAGAGGGTCAAT GCCCAGACAGCAGTGAGATCTCCTCGGTATGCTGTCTTTGACTTGGCGGAAGGGAAGTCCTATGTGTTCC GGGTTTTGTCAGCCAATAAGCATGGCCTGAGTGACCCATCAGAGATAACACCACCTATTCAGGCTCAGGA CATGATTGTGGTTCCCTCTGCACCTGGCCGGGTGCTTGCTTCCAGGAATACAAAGACATCTGTCGTGGTC CAGTGGGACAGACCAAAGCATGAAGAAGACCTGCTGGGCTACTATGTGGACTGCTGTGTGGCGGGAACAA ACATGTGGGAACCATGTAACCATAAACCCATTGGATATAACAGGTTCGTGGTACACGGCTTAACCACCGG TGAGCAGTACATCTTCCGGGTTAAGGCTGTGAATGCCGTGGGGACCAGCGAGAACTCCCAGGAGTCAGAG GTCATCAAGGTCCAGGCAGCCCTCACTGTCCCCTCTCATCCCTACGGGATTACCCTTCTCAACTGTGACG GTCACTCCATGACCCTGGGCTGGAAAGTGCCCAAGTTCAGCGGCGGCTCTGCCATCATAGGTTACTACCT GGACAAGCGGGAAGTGCATCATAAGAACTGGCACGAGATCAACTCTTCGCCTGTCAAAGAGAGGATCTTA ACGGTGGAAGGTTTGACTGAAGGCTCCTTGTATGAGTTCAAAATCGCTGCTACCAACCTGGCTGGGATCG GGCAGCCATCGGATCCCAGTGAGCACTTCAAGTGTGAGGCCTGGACAGCCCCAGAACCTGGTCCCGCCTA TGACTTGACGTTCTGTGAGGTCCGAGACACATCTCTGGTCATACTGTGGAAAGCCCCGGTGTACTCCGGC AGCAGTCCTGTTTCTGGATATTTTGTGGATTTTAAGGAAGAGGATTCTGGGGAGTGGAAGACCACCAGCG AGGCAGCAACTCCAAATCGCTACTTAAAGGTCTGTGACCTGCATCAAGGTAAGACCTATGTATTCAGAGT TCGAGCAGTGAATGCCAGTGGACCAGGGAAGCCGTCAGACACATCGGAGCCTGTGCTCGTGGAGGCCCGG CCAGGCACAAAGGAGATCAGTGCTGGTGTGGATGAGGAGGGCAACATCTACCTTGGGTTTGACTGCCAGG AAATGACAGACGCCTCGCAGTTCACCTGGTGCAAGGCCTATGAGGAGATCGCCGATGAGGAGCGGTTCGA GGTTCACACAGAGGGTGATCACTCAAAGCTGTACTTCAAGAATCCAGATAAAATAGACATAGGGACATAT TCTGTATCTGTAAGTGACACGGATGGTGTGTCTTCCAGCTTCGTTTTGGATGAGGAAGAACTTGAGCGTT TGATGGCGTTGAGCAATGAGATAAAGAATCCCACAATTCCTCTGAAATCAGAATTAGCATATGAGATTTT CGATAAGGGCCAGGTCCGATTCTGGCTCCAGGCAGAGCACCTCTCACCTGATGCCAACTTCCGGTTCATT ATTAATGACAGAGAAGTTTCTGACAGTGAGACACACAGAATTAAATGCGACAGATCGACAGGCATGATTG AGATGGTCATGGATCGGTTTACTATTGAAAACGAAGGGACCTACACTGTGCAGATTCAAGATGGAAAAGC CAAGAATCAGTCTTCGCTGGTGCTCATTGGAGATGCATTCAGGGCTGTCCTAGAAGAGGCTGAATTTCAA AGGAAGGAATTTCTACGGAAACAAGGCCCTCATTTTGCTGAGTATTTGCACTGGGATGTTACTGAAGAGT GTGAAGTTCGGCTTATCTGTAAGGTTGCAAACACCAAGAGGGAAACCGTTTTCAAATGGCTCAAGGATGA TGTCCTTTACGAAACCGAGACACCACCGCCGGACCTGGAGAAGGGGATCTGTGAGCTGCTTATACCCAAG TTGTCTAAGAAGGACCATGGCGAGTACAAAGCAACCTTGAAAGATGACAGAGGTCAAGATGTATCTGTCT TGGAAGTAGCTGGCAAAGTGTACGAGGACATGATCTTGGCAATGAGCCGAGTGTGTGGAGCCTCTGCCTC ACCCCTGAAGGTTCTCTGCACTCCAGAGGGCATCCGGCTTCAGTGCTTCATGAAGTATTTCACAGAGGAA ATGAAGGTCAGCTGGTACCACAAAGAGGCTAAGATCTCATCCAGTGAGCACATGCGGATCGGAGGAAGCG AGGAGATGGCCTGGCTGCAGATCTGTGAGCCCACAGAGAAGGACAAGGGGAAATACACTTTCGAGATCTT CGATGGCAAAGACAACCACCAACGCTCACTGGACCTCTCTGGGCAAGCCTTCGATGAAGCATATGCCGAG TTCCAGCAGCTCAAGGCTGCTGCATTTGCTGAGAAGAATCGTGGCAAGGTGATTGGCGGTTTGCCCGACG TGGTGACCATAATGGAAGGCAAGACCTTGAACCTCACCTGCACAGTATTTGGAAACCCGGACCCTGAGGT GGTCTGGTTTAAGAACGACAAGGACATTGAGCTAAGCGAGCACTTCTCGGTGAAGGTGGAGCAGTCCAAG TACGTCAGCCTGACCATTAAGGGGGTGACTGCTGAGGACTCGGGCAAGTACAGCATCAACGTGAAGAACA AATACGGAGGAGAGAAGATCGATGTCACCGTCAGCGTGTACAAACACGGGGAGAAGATCCCCGACATCTC AGCTCCCCAGCAGGCCAAACCCAAGCTTATCCCAGCTTCCACCTCAGCTGATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001169141
Insert Size	4395 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001169141.1, NP_001162612.1
RefSeq Size	4513 bp
RefSeq ORF	4395 bp
Locus ID	306616
Gene Summary	a major M-line structural protein [RGD, Feb 2006]

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