Uaca (NM_001195564) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1560011
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217613 representing NM_001195564 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGCCTCAAGTCCCGGCTGTGGAGGCAGGACGCGCCCGGTCCCACGTCACCCACATCTCCCACCG CGGTGACCAGTTCGCTTTCAGCAGAGTGGAATAAATACGATGACCGGCTGATGAAGGCTGCTGAAAGGGG GGATGTGGAGAAAGTATCCTCTATCCTTGCCAAAAAGGGCGTCCATCCAGGCAAGCTGGATGTGGAAGGC AGATCGGCCTTCCACGTCGTGGCAGCCAAAGGGAATCTGGAGTGTCTGAACGCCATTCTTACTCATGGCA TTGATGTTGCAACCAGGGACAGTGCAGGGAGGAATGCGCTTCACCTGGCAGCTAAGTACGGACACGCGTT GTGCCTACAGAAACTTCTACAGTACAACTGTCCCACTGAGCATGTGGACCTTCAGGGAAGAACCGCCCTA CACGACGCAGCAATGGCGGACTGTCCTTCCAGCATCCAGCTGCTTTGTGACCATGGGGCCTCCGTGAATG CCAAAGATATAGACGGGCGGACACCACTTGTTCTGGCGACTCAGATGTGCAGGCCGACAATTTGTCAGTT GCTGATAGACAGAGGGGCGGATGTCAATTCCAGAGACAAACAAAACAGGACAGCCCTCATGCTGGGCTGT GAGTACGGTTGCAGAGACGCTGTAGAAGTCCTCGTGAGAAATGGGGCCGACTTGACCTTGCTGGATGCCC TTGGCCATGACAGTTCTTACTACGCGAGAATTGGTGACAATCTGGACATCCTGAACCTGCTGAAGACAGC GTCAGAAAGCACCAGCAAAGGTGGCAGGAGAGAACTTTGGAGGAAAGGACCGCCTTTGCAACAGCGGAAT TTGTCACACACTCAAGATGAAGGAAATGTGAAGTCAAATCAGAGAGAACACCACAGCTTTCAGGATCTAG AGATGGAAAATGAAGATTTGAAAGAGAAGTTGAGGAAGATTCAGCAAGAGCAAAGAATACTCCTGGATAA AGTCAACGGCTTACAGTTACAGCTGAATGAGGAAGTCATGGTTGCAGATGATCTGGAAAGCGAGAGAGAA AAGCTGAAGTCCCTTTTGGCAGCTAAAGAAAAGCAGCACGAAGAAAGCTTACGAACCATCGAGGCCCTGA AAAACAGATTTAAATATTTTGAGAGTGATCATCCAGGACCCGGAAGTTACTCGAGTAACCGAAAAGAAGA CATGCTTCATAAACAAGGTCAAATGTACACGGCAGATTCACAGTGTACATCACCGGGCGTACCCCCCCAT ATGCAGAGTAGGTCTATGTTAAGGCCTCTGGAGTTGTCTTTACCAAGTCAGACCTCATACTCTGAAAATG AGATTTTAAAGAAAGAGTTGGAAGCTATGCGAACTTTCTGTGACTCGGCGAAACAGGACCGACTGAAGCT CCAAAACGAGTTGGCGCACAAGGTTGCGGAGTGCAAAGCCTTGGCTCTGGAGTGCGAGAGAGTCAAGGAG GACTCAGACGAGCAGATCAAGCAGCTGGAAGATGCCCTCAAAGATGTGCAGAAGAGGATGTATGAGTCGG AAGGCAAAGTCAAGCAAATGCAGACCCACTTCCTGGCCCTCAAAGAGCACCTGACCAATGAAGCAGCCAC GGGGAGCCACCGCGTCATCGAGGAGCTGAGGGAGCAGCTGAAAGACATGAAAGGGAAATATGAAGGCGCT TCAGCAGAAGTGGGCAAGCTGAGGAACCAAATCAAACAGAGCGAGATGCTGGTAGAAGGGTTCAAGAGGG ACGAGAGCAGGCTGGTGGAAGAGAATAAGAGATTGCAGAAGGAATGCAGCACGTGTGAAATAGATCGGGA GAGGAGGGGGCGGAGGGTCACGGAGCTGGAGGGCCAGCTCAAAGAGCTGGGGGCTAAGCTGGCCCTTTCC GTGCCCACAGAGACATTTGAAAGCATGAAGAGCTCGTTATCCAATGACATCAGTGAGAAAGCGAAAAGGC TAGCGGAGGTGGGGCGGGACTATGAAAGCTCTCAGGGGGAGATTCGACAGCTGAAGAGGGATCTGGAGAG CGTTAGGGCCCAGCACATCAGACCGGAGGAGCATGAGCAGCTCAGGAGCAGGTTGGAGCAGAAGTCGGGA GAGCTGGGGAAGAAAGTGACAGAGCTAACCCTGAAAAACCAGACACTCCAGAAAGAAGTGGAAAAACTCT ACGCAGACAACAAACTCCTCAACCAGCAGGTGCACAGCCTCACGGTGGAGATGAAAACTCGCTATGTGCC TTTGAGGGTCAGCGAGGAGATGAAGAAGGCGCATGATGTGAATGTGGAGGATTTGAATAAGAAGCTTTCG GATGCAACACAGAGATACGCAGAGAAGAAGCTAGAAGCAGAGAGACTGCTGGCAGAGAACGATAAGTTGA CCAAGAACGTCAGCCGCCTGGAAGCTGTGTTTGTAGCTCCCGAGAAACATGAAAAGGAGCTAATGGGTCT GAAATCCAATATCGCTGAACTAAAAATGCAGCTGTGTGAGCTTAATAAAAAATGTGGTGAGGGTCAGGAG AAAATACGTGCGCTTATGTCTGAAAACACCAGCTTGAAAAAGACTCTGAGTAGCCAGTACGTGCCTGCCA AAACCCACGAGGAGGTGAAGGCCTCCCTGAGCAGCACTTTGGAGAAAACCAACAGAGCTTTATTGGACTC GAAGAAAAGGCTTGACGACACGAGCCAGGAATTTTCAAAACTAAGGGAGGAGAATGAAGTGTTGAGGAGG AACCTGGAGAACGTTCAGAATCAGATGAAGGCTGACTACGTGAGCCTGGAGGAACACTCGAGAAAGATGA ACATGGCGAACCAGAGCCTGAAGGAGGCGCAGGATGCCCACACTGCCCTCCTGGCTGACTACCGCCAGGG CCAGGAGGAGATCGTCACGCTGCACGCAGAAATTAAGGCCCAGAAAAAGGAGCTCGACACCATCCAAGAA TGCATCAAGCTAAAATATGCTCCCCTCTCCCGAATGGAAGAGTGTGAACGCAAGTTCAAGGCTACAGAGA AAGCACTGAAGGAGCAGTTATCAGAGCAGACACACAAGGGCCAGGTGAGGGACGAAGAGGTCAAGAAGGG CAAGCAAGAGAATGAGAGGCTGAGGAAGGATCTCGCCGCCCTTCAGAAGGAGCTGAAGGATAGGAATGTT CTGGTGGAAGAGGCCAGGGAGGCAGAAAGAGCACTGAGCAGGAAGACAGAAGAACTCGGCAAACAGCTGA AGGAACTGTCCCAGAAGTACAGCGATGTGAAGAGCGAGAGAGAGAAGCTGGTGGAAGAAAAGGCCAAGCA GACTTCCGAGATCCTTGCAGCCCAAAATCTTCTGCAAAAACAGCCAGTCCCACTGGAGCAGGTGGAGGCT CTCAAAACATCTCTCAACGGCACAATCGAGCACCTAAAGGAGGAGCTGAGGAGTAAGGAGAGGTGTCTGG AGAGAGAGCAGCAGGCGGTGAGCCAACTTCAGCAGCTGCTGGAGAATCAGAAGAGCTCCTCCGTGACCCT GGCGGATCACCTGCAGCTAAAGGAAGCCTTGGAGAAAGAGGTTGGGATCATGAAAGCCAGCCTGAGAGAG AAGGAAGAGGAAAGCCAGAAGAAAACCAAAGAAGTCTCCAAACTCCAGACCGAGGTCCAGAACACCAAGC AGGCGCTGAAGAACTTAGAGACCAGAGAGGTCGTCGACATGTCAAAATACAAAGCCACCAAGAATGATTT GGAGACCCAGATTTCCAACTTAAATGACAAACTGGCCAGTCTGAACAGGAAGTACGACCAAGTGTGTGAG GAGAAGGTCTCTGCCAAGGACGAGAAGGAGCTGCTGCATCTTAACATCGAGCAGGAGATCAGGGACCAGA AGGAGCGGTGTGACAAGTCCCTGACAACCATCATGGAGCTGCAGCAGAGGATACAGGAGTCCGCCAAGCA GATCGAAGCAAAGGACAATAAGATAACTGAACTTCTGAATGATGTCGAAAGACTAAAGCAGGCGCTCAAT GGCCTTTCCCAGCTCACCTACAGCAGCGGCGGCCCCACCAAGCGGCAGAGCCAGCTAGTGGACACCCTGC AGCAGCGAGTGAGGGATCTGCAGCAGCAGCTGGCTGATGCTGACAGACAGCACCAGGAAGTGATCGCTAT CTACCGGACACACCTCCTCAGTGCCGCACAGGGTCACATGGATGAAGATGTGCAGGCAGCCTTGCTGCAG ATCATACAGATGCGACAAGGCCTCGTGTGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217613 representing NM_001195564 Red=Cloning site Green=Tags(s) MKSLKSRLWRQDAPGPTSPTSPTAVTSSLSAEWNKYDDRLMKAAERGDVEKVSSILAKKGVHPGKLDVEG RSAFHVVAAKGNLECLNAILTHGIDVATRDSAGRNALHLAAKYGHALCLQKLLQYNCPTEHVDLQGRTAL HDAAMADCPSSIQLLCDHGASVNAKDIDGRTPLVLATQMCRPTICQLLIDRGADVNSRDKQNRTALMLGC EYGCRDAVEVLVRNGADLTLLDALGHDSSYYARIGDNLDILNLLKTASESTSKGGRRELWRKGPPLQQRN LSHTQDEGNVKSNQREHHSFQDLEMENEDLKEKLRKIQQEQRILLDKVNGLQLQLNEEVMVADDLESERE KLKSLLAAKEKQHEESLRTIEALKNRFKYFESDHPGPGSYSSNRKEDMLHKQGQMYTADSQCTSPGVPPH MQSRSMLRPLELSLPSQTSYSENEILKKELEAMRTFCDSAKQDRLKLQNELAHKVAECKALALECERVKE DSDEQIKQLEDALKDVQKRMYESEGKVKQMQTHFLALKEHLTNEAATGSHRVIEELREQLKDMKGKYEGA SAEVGKLRNQIKQSEMLVEGFKRDESRLVEENKRLQKECSTCEIDRERRGRRVTELEGQLKELGAKLALS VPTETFESMKSSLSNDISEKAKRLAEVGRDYESSQGEIRQLKRDLESVRAQHIRPEEHEQLRSRLEQKSG ELGKKVTELTLKNQTLQKEVEKLYADNKLLNQQVHSLTVEMKTRYVPLRVSEEMKKAHDVNVEDLNKKLS DATQRYAEKKLEAERLLAENDKLTKNVSRLEAVFVAPEKHEKELMGLKSNIAELKMQLCELNKKCGEGQE KIRALMSENTSLKKTLSSQYVPAKTHEEVKASLSSTLEKTNRALLDSKKRLDDTSQEFSKLREENEVLRR NLENVQNQMKADYVSLEEHSRKMNMANQSLKEAQDAHTALLADYRQGQEEIVTLHAEIKAQKKELDTIQE CIKLKYAPLSRMEECERKFKATEKALKEQLSEQTHKGQVRDEEVKKGKQENERLRKDLAALQKELKDRNV LVEEAREAERALSRKTEELGKQLKELSQKYSDVKSEREKLVEEKAKQTSEILAAQNLLQKQPVPLEQVEA LKTSLNGTIEHLKEELRSKERCLEREQQAVSQLQQLLENQKSSSVTLADHLQLKEALEKEVGIMKASLRE KEEESQKKTKEVSKLQTEVQNTKQALKNLETREVVDMSKYKATKNDLETQISNLNDKLASLNRKYDQVCE EKVSAKDEKELLHLNIEQEIRDQKERCDKSLTTIMELQQRIQESAKQIEAKDNKITELLNDVERLKQALN GLSQLTYSSGGPTKRQSQLVDTLQQRVRDLQQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQAALLQ IIQMRQGLVC SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001195564
ORF Size	4230 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195564.1, NP_001182493.1
RefSeq Size	4422 bp
RefSeq ORF	4233 bp
Locus ID	315732
MW	160.9 kDa

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