Uaca (NM_001195564) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1560011
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217613 representing NM_001195564 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGCCTCAAGTCCCGGCTGTGGAGGCAGGACGCGCCCGGTCCCACGTCACCCACATCTCCCACCG CGGTGACCAGTTCGCTTTCAGCAGAGTGGAATAAATACGATGACCGGCTGATGAAGGCTGCTGAAAGGGG GGATGTGGAGAAAGTATCCTCTATCCTTGCCAAAAAGGGCGTCCATCCAGGCAAGCTGGATGTGGAAGGC AGATCGGCCTTCCACGTCGTGGCAGCCAAAGGGAATCTGGAGTGTCTGAACGCCATTCTTACTCATGGCA TTGATGTTGCAACCAGGGACAGTGCAGGGAGGAATGCGCTTCACCTGGCAGCTAAGTACGGACACGCGTT GTGCCTACAGAAACTTCTACAGTACAACTGTCCCACTGAGCATGTGGACCTTCAGGGAAGAACCGCCCTA CACGACGCAGCAATGGCGGACTGTCCTTCCAGCATCCAGCTGCTTTGTGACCATGGGGCCTCCGTGAATG CCAAAGATATAGACGGGCGGACACCACTTGTTCTGGCGACTCAGATGTGCAGGCCGACAATTTGTCAGTT GCTGATAGACAGAGGGGCGGATGTCAATTCCAGAGACAAACAAAACAGGACAGCCCTCATGCTGGGCTGT GAGTACGGTTGCAGAGACGCTGTAGAAGTCCTCGTGAGAAATGGGGCCGACTTGACCTTGCTGGATGCCC TTGGCCATGACAGTTCTTACTACGCGAGAATTGGTGACAATCTGGACATCCTGAACCTGCTGAAGACAGC GTCAGAAAGCACCAGCAAAGGTGGCAGGAGAGAACTTTGGAGGAAAGGACCGCCTTTGCAACAGCGGAAT TTGTCACACACTCAAGATGAAGGAAATGTGAAGTCAAATCAGAGAGAACACCACAGCTTTCAGGATCTAG AGATGGAAAATGAAGATTTGAAAGAGAAGTTGAGGAAGATTCAGCAAGAGCAAAGAATACTCCTGGATAA AGTCAACGGCTTACAGTTACAGCTGAATGAGGAAGTCATGGTTGCAGATGATCTGGAAAGCGAGAGAGAA AAGCTGAAGTCCCTTTTGGCAGCTAAAGAAAAGCAGCACGAAGAAAGCTTACGAACCATCGAGGCCCTGA AAAACAGATTTAAATATTTTGAGAGTGATCATCCAGGACCCGGAAGTTACTCGAGTAACCGAAAAGAAGA CATGCTTCATAAACAAGGTCAAATGTACACGGCAGATTCACAGTGTACATCACCGGGCGTACCCCCCCAT ATGCAGAGTAGGTCTATGTTAAGGCCTCTGGAGTTGTCTTTACCAAGTCAGACCTCATACTCTGAAAATG AGATTTTAAAGAAAGAGTTGGAAGCTATGCGAACTTTCTGTGACTCGGCGAAACAGGACCGACTGAAGCT CCAAAACGAGTTGGCGCACAAGGTTGCGGAGTGCAAAGCCTTGGCTCTGGAGTGCGAGAGAGTCAAGGAG GACTCAGACGAGCAGATCAAGCAGCTGGAAGATGCCCTCAAAGATGTGCAGAAGAGGATGTATGAGTCGG AAGGCAAAGTCAAGCAAATGCAGACCCACTTCCTGGCCCTCAAAGAGCACCTGACCAATGAAGCAGCCAC GGGGAGCCACCGCGTCATCGAGGAGCTGAGGGAGCAGCTGAAAGACATGAAAGGGAAATATGAAGGCGCT TCAGCAGAAGTGGGCAAGCTGAGGAACCAAATCAAACAGAGCGAGATGCTGGTAGAAGGGTTCAAGAGGG ACGAGAGCAGGCTGGTGGAAGAGAATAAGAGATTGCAGAAGGAATGCAGCACGTGTGAAATAGATCGGGA GAGGAGGGGGCGGAGGGTCACGGAGCTGGAGGGCCAGCTCAAAGAGCTGGGGGCTAAGCTGGCCCTTTCC GTGCCCACAGAGACATTTGAAAGCATGAAGAGCTCGTTATCCAATGACATCAGTGAGAAAGCGAAAAGGC TAGCGGAGGTGGGGCGGGACTATGAAAGCTCTCAGGGGGAGATTCGACAGCTGAAGAGGGATCTGGAGAG CGTTAGGGCCCAGCACATCAGACCGGAGGAGCATGAGCAGCTCAGGAGCAGGTTGGAGCAGAAGTCGGGA GAGCTGGGGAAGAAAGTGACAGAGCTAACCCTGAAAAACCAGACACTCCAGAAAGAAGTGGAAAAACTCT ACGCAGACAACAAACTCCTCAACCAGCAGGTGCACAGCCTCACGGTGGAGATGAAAACTCGCTATGTGCC TTTGAGGGTCAGCGAGGAGATGAAGAAGGCGCATGATGTGAATGTGGAGGATTTGAATAAGAAGCTTTCG GATGCAACACAGAGATACGCAGAGAAGAAGCTAGAAGCAGAGAGACTGCTGGCAGAGAACGATAAGTTGA CCAAGAACGTCAGCCGCCTGGAAGCTGTGTTTGTAGCTCCCGAGAAACATGAAAAGGAGCTAATGGGTCT GAAATCCAATATCGCTGAACTAAAAATGCAGCTGTGTGAGCTTAATAAAAAATGTGGTGAGGGTCAGGAG AAAATACGTGCGCTTATGTCTGAAAACACCAGCTTGAAAAAGACTCTGAGTAGCCAGTACGTGCCTGCCA AAACCCACGAGGAGGTGAAGGCCTCCCTGAGCAGCACTTTGGAGAAAACCAACAGAGCTTTATTGGACTC GAAGAAAAGGCTTGACGACACGAGCCAGGAATTTTCAAAACTAAGGGAGGAGAATGAAGTGTTGAGGAGG AACCTGGAGAACGTTCAGAATCAGATGAAGGCTGACTACGTGAGCCTGGAGGAACACTCGAGAAAGATGA ACATGGCGAACCAGAGCCTGAAGGAGGCGCAGGATGCCCACACTGCCCTCCTGGCTGACTACCGCCAGGG CCAGGAGGAGATCGTCACGCTGCACGCAGAAATTAAGGCCCAGAAAAAGGAGCTCGACACCATCCAAGAA TGCATCAAGCTAAAATATGCTCCCCTCTCCCGAATGGAAGAGTGTGAACGCAAGTTCAAGGCTACAGAGA AAGCACTGAAGGAGCAGTTATCAGAGCAGACACACAAGGGCCAGGTGAGGGACGAAGAGGTCAAGAAGGG CAAGCAAGAGAATGAGAGGCTGAGGAAGGATCTCGCCGCCCTTCAGAAGGAGCTGAAGGATAGGAATGTT CTGGTGGAAGAGGCCAGGGAGGCAGAAAGAGCACTGAGCAGGAAGACAGAAGAACTCGGCAAACAGCTGA AGGAACTGTCCCAGAAGTACAGCGATGTGAAGAGCGAGAGAGAGAAGCTGGTGGAAGAAAAGGCCAAGCA GACTTCCGAGATCCTTGCAGCCCAAAATCTTCTGCAAAAACAGCCAGTCCCACTGGAGCAGGTGGAGGCT CTCAAAACATCTCTCAACGGCACAATCGAGCACCTAAAGGAGGAGCTGAGGAGTAAGGAGAGGTGTCTGG AGAGAGAGCAGCAGGCGGTGAGCCAACTTCAGCAGCTGCTGGAGAATCAGAAGAGCTCCTCCGTGACCCT GGCGGATCACCTGCAGCTAAAGGAAGCCTTGGAGAAAGAGGTTGGGATCATGAAAGCCAGCCTGAGAGAG AAGGAAGAGGAAAGCCAGAAGAAAACCAAAGAAGTCTCCAAACTCCAGACCGAGGTCCAGAACACCAAGC AGGCGCTGAAGAACTTAGAGACCAGAGAGGTCGTCGACATGTCAAAATACAAAGCCACCAAGAATGATTT GGAGACCCAGATTTCCAACTTAAATGACAAACTGGCCAGTCTGAACAGGAAGTACGACCAAGTGTGTGAG GAGAAGGTCTCTGCCAAGGACGAGAAGGAGCTGCTGCATCTTAACATCGAGCAGGAGATCAGGGACCAGA AGGAGCGGTGTGACAAGTCCCTGACAACCATCATGGAGCTGCAGCAGAGGATACAGGAGTCCGCCAAGCA GATCGAAGCAAAGGACAATAAGATAACTGAACTTCTGAATGATGTCGAAAGACTAAAGCAGGCGCTCAAT GGCCTTTCCCAGCTCACCTACAGCAGCGGCGGCCCCACCAAGCGGCAGAGCCAGCTAGTGGACACCCTGC AGCAGCGAGTGAGGGATCTGCAGCAGCAGCTGGCTGATGCTGACAGACAGCACCAGGAAGTGATCGCTAT CTACCGGACACACCTCCTCAGTGCCGCACAGGGTCACATGGATGAAGATGTGCAGGCAGCCTTGCTGCAG ATCATACAGATGCGACAAGGCCTCGTGTGCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001195564
Insert Size	4233 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001195564.1, NP_001182493.1
RefSeq Size	4422 bp
RefSeq ORF	4233 bp
Locus ID	315732

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