Ppfia4 (NM_080409) Rat Tagged ORF Clone
CAT#: RR217531
- TrueORF®
Ppfia4 (myc-DDK-tagged) - Rat protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (Ppfia4)
"NM_080409" in other vectors (1)
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CNY 11,500.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR217531 representing NM_080409
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGAGGTGATGCCCACAATCAATGAGGGGGACCCTCTGGGGCCCCCCCACGGTGCCGATGCTGACG CCAACTTTGAGCAGTTGATGGTGAACATGCTGGATGAGCGAGAGAAGTTGCTGGAGTCCCTTCGGGAGAG TCAGGAAACTTTGGTAGCCACACAGAGCCGGCTCCAAGATGCCCTTCACGAGCGAGACCAGCTCCAGCGC CACCTGAACTCTGCCCTTCCCCAGGAATTTGCCACATTAACTCGGGAGCTAAGCATGTGTCGGGAGCAGC TTCTGGAGCGGGAGGAAGAGATATCTGAGCTGAAAGCAGAACGGAATAACACTAGGCTGCTTCTGGAACA TCTGGAATGCCTCGTGTCCCGCCATGAGCGGTCATTGCGAATGACAGTGGTAAAACGCCAGGCCCAGTCA CCTTCAGGTGTCTCCAGTGAGGTGGAGGTGCTGAAGGCCCTCAAGTCACTATTTGAGCACCACAAGGCCC TGGATGAGAAGGTTCGAGAGCGGCTCCGGGCAGCTCTAGAGCGAGTTACCACCTTGGAGGAGCAGTTGGC AGGAGCTCACCAGCAGGTGTCTGCCCTGCAGCAGGGGGCAGGAATTCGGGATGGAGTGGCAGAAGAGGAA GAAACTGTGGATCTGGGACCAAAACGCCTGTGGAAAGATGACACTGGCCGTGTGGAAGAACTGCAAGGGC TCTTGGAGAAGCAAAACTATGAGTTGAGCCAGGCCCGGGAGCGGCTGGTCACCCTGAGTGCAACTGTGAC TGAGCTTGAGGAGGACCTGGGCACAGCCCGCCGAGACCTCATCAAGTCAGAGGAGCTGAGTGGCAAGCAT CAGCGCGACCTCCGAGAGGCTCTGGCTCAGAAGGAGGACATGGAAGAGCGAATCACCACCCTGGAGAAGC GCTACTTGGCTGCTCAGCGAGAGGCCACATCCATCCATGACCTCAATGACAAACTGGAGAATGAGCTAGC CAACAAGGAGTCCCTGCACCGCCAGTGTGAGGAGAAAGCCCGGCATCTGCAGGAGCTGTTGGAAGTGGCA GAGCAGAAGCTGCAGCAGACCATGCGCAAGGCAGAGACCCTGCCAGAGGTGGAGGCCGAGCTGTCTCAGA GAATTGCAGCTCTCACCAAGGCTGAGGAACGTCATGGCAACATTGAGGAACATCTGCGGCAGCTGGAAGG GCAGCTGGAGGAGAAGAACCAGGAGCTGGCAAGGGTGCGCCAGCGGGAGAAGATGAACGAGGACCACAAC AAGCGGCTGTCAGACACTGTGGACCGGTTGCTTAGTGAGTCCAACGAGCGCCTGCAGCTCCACCTCAAGG AGCGAATGGCTGCCCTGGAGGAGAAGGGCCGTCTGTCTGAGGAGATTGAGAAGCTTCGCCAAGAGGTGGA CCAGCTGAAGGGTCGAGGGGGGCCGTTTGTGGATGGCATCCACTCCAGGTCACACGTGGGCAGTACAACA GATGTGCGGTTCTCACTGAGTACAGCCGCGCACGTGCCTCCAGGTCTGCATCGTCGCTATACGGCTCTGA GGGAAGAGTCTGCCAAGGACTGGAAGCCGGCACCACTGCCGGGGGTGTTGGCTGCCACCACCACGCCTGC CTTTGACAGTGACCCTGAGATCTCCGATGTGGACGAGGATGAGCCTGGTGGCTTGGTGGGCACTCAGGTT GATGTCATCTCACCTGGTGGCCATTCTGATGCCCAAACCCTGGCCATGATGCTCCAGGAACAACTGGATG CCATCAACCAGGAGATCAGGATGATTCAGGAGGAGAAGGAATCCACTGAGCTGCGTGCTGAAGAGATTGA GACCCGTGTGACCAGCGGCAGCATGGAGGCCCTCAACCTGACCCAGCTGCGCAAGCGTGGCTCCATCCCC ACCTCTCTGACCGCCCTGTCCCTGGCCAGTGCATCTCCACCACTCAGTGGCCGCTCCACACCCAAGCTCA CTTCCAGAAGTGCTGCCCAGGACCTGGACCGAATGGGGGTCATGACCCTGCCCAGCGACTTAAGAAAGCA TAGGAGGAAGCTGCTGTCGCCAGTGTCTCGGGAAGAGAACCGAGAGGATAAAGCCACCATAAAATGTGAG ACTTCTCCTCCTTCCTCACCCAGGACGCTGCGGCTAGAGAAGCTTGGCCACCCTACCCTGAGTCAAGAAG AAGGCAAGAGTGCCTTGGAGGGCCAGGACAGCAACCCCAGCAGCAGCAACAGCAGCCAGGACTCCCTGCA CAAGGGCGCCAAGCGCAAGGGCATCAAGTCGTCCATTGGCCGCCTGTTTGGGAAGAAGGAGAAGGGCAGG CTGATCCACTTGAGTCGGGATGCCACAGGCCATGTTCTGCTCACAGACTCCGAGCTCAGTCTGCAGGAAC CAATGGTACCTGCCAAATTGGGGACCCAGGCAGAGAAGGACCGGCGACTGAAAAAGAAACACCAGCTGCT TGAAGATGCTCGCAGAAAAGGAATGCCTTTTGCCCAATGGGATGGCCCCACGGTGGTCTCCTGGCTGGAG CTCTGGGTGGGGATGCCTGCTTGGTATGTGGCGGCCTGCCGGGCCAATGTCAAGAGTGGAGCCATCATGT CTGCCCTGTCAGACACGGAGATCCAGCGGGAGATCGGCATCAGCAATGCCCTGCACAGGCTCAAGCTCCG CCTGGCCATCCAGGAGATGGTGTCACTGACGAGCCCCTCAGCCCCGCCTACCTCAAGGACATCTTCAGGG AATGTCTGGGTGACCCACGAAGAGATGGAAACTCTGGCAACGTCTACCAAAACAGACAGTGAGGAGGGCA GTTGGGCCCAGACCCTGGCCTACGGAGATATGAATCACGAATGGATTGGAAATGAATGGCTGCCCAGCCT GGGGCTCCCTCAGTACCGCAGTTACTTCATGGAGTGCCTAGTGGATGCACGCATGCTGGATCACCTCACC AAGAAGGACCTGCGTGTCCACCTCAAGATGGTGGACAGTTTCCATAGAACCAGTCTTCAGTATGGCATCA TGTGTCTGAAGAGGCTGAACTATGACCGGAAGGAGCTGGAGAAGAGGCGGGAAGAGAGCCAGCATGAGAT CAAGGATGTGCTGGTCTGGACCAATGACCAGGTGGTTCACTGGGTCCAGTCCATAGGGCTAAGGGATTAT GCCGGAAACCTACATGAAAGTGGTGTGCATGGTGCCCTGCTGGCCTTGGATGAAAACTTCGACCACAACA CATTGGCCTTGGTTCTGCAGATCCCCACACAGAACACCCAGGCCCGCCAAGTGATGGAGAGAGAGTTCAA TAACCTGTTGGCCTTGGGCACAGACAGGAAATTGGATGACGGGGAAGAGAAGGTGTTCCGTCGAGCACCC TCCTGGAGGAAACGCTTTCGGCCTCGGGATCACCATAGCGGTGGCATGTTGGGCACCTCAGCGGAGACCC TCCCTGCGGGCTTCCGTGTGTCCACCCTGGGTCCGCTGCAGCCTCCACCCGCCCCGCCCAACAAGATCAT GCCGGAAGCTCACTCCCACTATCTCTACGGACACATGCTCTCCGCCTTCCGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR217531 representing NM_080409
Red=Cloning site Green=Tags(s) MCEVMPTINEGDPLGPPHGADADANFEQLMVNMLDEREKLLESLRESQETLVATQSRLQDALHERDQLQR HLNSALPQEFATLTRELSMCREQLLEREEEISELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQS PSGVSSEVEVLKALKSLFEHHKALDEKVRERLRAALERVTTLEEQLAGAHQQVSALQQGAGIRDGVAEEE ETVDLGPKRLWKDDTGRVEELQGLLEKQNYELSQARERLVTLSATVTELEEDLGTARRDLIKSEELSGKH QRDLREALAQKEDMEERITTLEKRYLAAQREATSIHDLNDKLENELANKESLHRQCEEKARHLQELLEVA EQKLQQTMRKAETLPEVEAELSQRIAALTKAEERHGNIEEHLRQLEGQLEEKNQELARVRQREKMNEDHN KRLSDTVDRLLSESNERLQLHLKERMAALEEKGRLSEEIEKLRQEVDQLKGRGGPFVDGIHSRSHVGSTT DVRFSLSTAAHVPPGLHRRYTALREESAKDWKPAPLPGVLAATTTPAFDSDPEISDVDEDEPGGLVGTQV DVISPGGHSDAQTLAMMLQEQLDAINQEIRMIQEEKESTELRAEEIETRVTSGSMEALNLTQLRKRGSIP TSLTALSLASASPPLSGRSTPKLTSRSAAQDLDRMGVMTLPSDLRKHRRKLLSPVSREENREDKATIKCE TSPPSSPRTLRLEKLGHPTLSQEEGKSALEGQDSNPSSSNSSQDSLHKGAKRKGIKSSIGRLFGKKEKGR LIHLSRDATGHVLLTDSELSLQEPMVPAKLGTQAEKDRRLKKKHQLLEDARRKGMPFAQWDGPTVVSWLE LWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNALHRLKLRLAIQEMVSLTSPSAPPTSRTSSG NVWVTHEEMETLATSTKTDSEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLT KKDLRVHLKMVDSFHRTSLQYGIMCLKRLNYDRKELEKRREESQHEIKDVLVWTNDQVVHWVQSIGLRDY AGNLHESGVHGALLALDENFDHNTLALVLQIPTQNTQARQVMEREFNNLLALGTDRKLDDGEEKVFRRAP SWRKRFRPRDHHSGGMLGTSAETLPAGFRVSTLGPLQPPPAPPNKIMPEAHSHYLYGHMLSAFRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080409 |
ORF Size | 3555 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080409.1, NP_536334.1 |
RefSeq Size | 5852 bp |
RefSeq ORF | 3558 bp |
Locus ID | 140592 |
UniProt ID | Q91Z80 |
MW | 133.9 kDa |
Gene Summary | putative effectors for Rab3A, a synaptic vesicle protein that regulates synaptic vesicle exocytosis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN217531 | Ppfia4 (untagged) - Rat protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 (Ppfia4) |
CNY 11,500.00 |