Ppfia4 (NM_080409) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217531 representing NM_080409 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGAGGTGATGCCCACAATCAATGAGGGGGACCCTCTGGGGCCCCCCCACGGTGCCGATGCTGACG CCAACTTTGAGCAGTTGATGGTGAACATGCTGGATGAGCGAGAGAAGTTGCTGGAGTCCCTTCGGGAGAG TCAGGAAACTTTGGTAGCCACACAGAGCCGGCTCCAAGATGCCCTTCACGAGCGAGACCAGCTCCAGCGC CACCTGAACTCTGCCCTTCCCCAGGAATTTGCCACATTAACTCGGGAGCTAAGCATGTGTCGGGAGCAGC TTCTGGAGCGGGAGGAAGAGATATCTGAGCTGAAAGCAGAACGGAATAACACTAGGCTGCTTCTGGAACA TCTGGAATGCCTCGTGTCCCGCCATGAGCGGTCATTGCGAATGACAGTGGTAAAACGCCAGGCCCAGTCA CCTTCAGGTGTCTCCAGTGAGGTGGAGGTGCTGAAGGCCCTCAAGTCACTATTTGAGCACCACAAGGCCC TGGATGAGAAGGTTCGAGAGCGGCTCCGGGCAGCTCTAGAGCGAGTTACCACCTTGGAGGAGCAGTTGGC AGGAGCTCACCAGCAGGTGTCTGCCCTGCAGCAGGGGGCAGGAATTCGGGATGGAGTGGCAGAAGAGGAA GAAACTGTGGATCTGGGACCAAAACGCCTGTGGAAAGATGACACTGGCCGTGTGGAAGAACTGCAAGGGC TCTTGGAGAAGCAAAACTATGAGTTGAGCCAGGCCCGGGAGCGGCTGGTCACCCTGAGTGCAACTGTGAC TGAGCTTGAGGAGGACCTGGGCACAGCCCGCCGAGACCTCATCAAGTCAGAGGAGCTGAGTGGCAAGCAT CAGCGCGACCTCCGAGAGGCTCTGGCTCAGAAGGAGGACATGGAAGAGCGAATCACCACCCTGGAGAAGC GCTACTTGGCTGCTCAGCGAGAGGCCACATCCATCCATGACCTCAATGACAAACTGGAGAATGAGCTAGC CAACAAGGAGTCCCTGCACCGCCAGTGTGAGGAGAAAGCCCGGCATCTGCAGGAGCTGTTGGAAGTGGCA GAGCAGAAGCTGCAGCAGACCATGCGCAAGGCAGAGACCCTGCCAGAGGTGGAGGCCGAGCTGTCTCAGA GAATTGCAGCTCTCACCAAGGCTGAGGAACGTCATGGCAACATTGAGGAACATCTGCGGCAGCTGGAAGG GCAGCTGGAGGAGAAGAACCAGGAGCTGGCAAGGGTGCGCCAGCGGGAGAAGATGAACGAGGACCACAAC AAGCGGCTGTCAGACACTGTGGACCGGTTGCTTAGTGAGTCCAACGAGCGCCTGCAGCTCCACCTCAAGG AGCGAATGGCTGCCCTGGAGGAGAAGGGCCGTCTGTCTGAGGAGATTGAGAAGCTTCGCCAAGAGGTGGA CCAGCTGAAGGGTCGAGGGGGGCCGTTTGTGGATGGCATCCACTCCAGGTCACACGTGGGCAGTACAACA GATGTGCGGTTCTCACTGAGTACAGCCGCGCACGTGCCTCCAGGTCTGCATCGTCGCTATACGGCTCTGA GGGAAGAGTCTGCCAAGGACTGGAAGCCGGCACCACTGCCGGGGGTGTTGGCTGCCACCACCACGCCTGC CTTTGACAGTGACCCTGAGATCTCCGATGTGGACGAGGATGAGCCTGGTGGCTTGGTGGGCACTCAGGTT GATGTCATCTCACCTGGTGGCCATTCTGATGCCCAAACCCTGGCCATGATGCTCCAGGAACAACTGGATG CCATCAACCAGGAGATCAGGATGATTCAGGAGGAGAAGGAATCCACTGAGCTGCGTGCTGAAGAGATTGA GACCCGTGTGACCAGCGGCAGCATGGAGGCCCTCAACCTGACCCAGCTGCGCAAGCGTGGCTCCATCCCC ACCTCTCTGACCGCCCTGTCCCTGGCCAGTGCATCTCCACCACTCAGTGGCCGCTCCACACCCAAGCTCA CTTCCAGAAGTGCTGCCCAGGACCTGGACCGAATGGGGGTCATGACCCTGCCCAGCGACTTAAGAAAGCA TAGGAGGAAGCTGCTGTCGCCAGTGTCTCGGGAAGAGAACCGAGAGGATAAAGCCACCATAAAATGTGAG ACTTCTCCTCCTTCCTCACCCAGGACGCTGCGGCTAGAGAAGCTTGGCCACCCTACCCTGAGTCAAGAAG AAGGCAAGAGTGCCTTGGAGGGCCAGGACAGCAACCCCAGCAGCAGCAACAGCAGCCAGGACTCCCTGCA CAAGGGCGCCAAGCGCAAGGGCATCAAGTCGTCCATTGGCCGCCTGTTTGGGAAGAAGGAGAAGGGCAGG CTGATCCACTTGAGTCGGGATGCCACAGGCCATGTTCTGCTCACAGACTCCGAGCTCAGTCTGCAGGAAC CAATGGTACCTGCCAAATTGGGGACCCAGGCAGAGAAGGACCGGCGACTGAAAAAGAAACACCAGCTGCT TGAAGATGCTCGCAGAAAAGGAATGCCTTTTGCCCAATGGGATGGCCCCACGGTGGTCTCCTGGCTGGAG CTCTGGGTGGGGATGCCTGCTTGGTATGTGGCGGCCTGCCGGGCCAATGTCAAGAGTGGAGCCATCATGT CTGCCCTGTCAGACACGGAGATCCAGCGGGAGATCGGCATCAGCAATGCCCTGCACAGGCTCAAGCTCCG CCTGGCCATCCAGGAGATGGTGTCACTGACGAGCCCCTCAGCCCCGCCTACCTCAAGGACATCTTCAGGG AATGTCTGGGTGACCCACGAAGAGATGGAAACTCTGGCAACGTCTACCAAAACAGACAGTGAGGAGGGCA GTTGGGCCCAGACCCTGGCCTACGGAGATATGAATCACGAATGGATTGGAAATGAATGGCTGCCCAGCCT GGGGCTCCCTCAGTACCGCAGTTACTTCATGGAGTGCCTAGTGGATGCACGCATGCTGGATCACCTCACC AAGAAGGACCTGCGTGTCCACCTCAAGATGGTGGACAGTTTCCATAGAACCAGTCTTCAGTATGGCATCA TGTGTCTGAAGAGGCTGAACTATGACCGGAAGGAGCTGGAGAAGAGGCGGGAAGAGAGCCAGCATGAGAT CAAGGATGTGCTGGTCTGGACCAATGACCAGGTGGTTCACTGGGTCCAGTCCATAGGGCTAAGGGATTAT GCCGGAAACCTACATGAAAGTGGTGTGCATGGTGCCCTGCTGGCCTTGGATGAAAACTTCGACCACAACA CATTGGCCTTGGTTCTGCAGATCCCCACACAGAACACCCAGGCCCGCCAAGTGATGGAGAGAGAGTTCAA TAACCTGTTGGCCTTGGGCACAGACAGGAAATTGGATGACGGGGAAGAGAAGGTGTTCCGTCGAGCACCC TCCTGGAGGAAACGCTTTCGGCCTCGGGATCACCATAGCGGTGGCATGTTGGGCACCTCAGCGGAGACCC TCCCTGCGGGCTTCCGTGTGTCCACCCTGGGTCCGCTGCAGCCTCCACCCGCCCCGCCCAACAAGATCAT GCCGGAAGCTCACTCCCACTATCTCTACGGACACATGCTCTCCGCCTTCCGGGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_080409
Insert Size	3558 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_080409.1, NP_536334.1
RefSeq Size	5852 bp
RefSeq ORF	3558 bp
Locus ID	140592
UniProt ID	Q91Z80
Gene Summary	putative effectors for Rab3A, a synaptic vesicle protein that regulates synaptic vesicle exocytosis [RGD, Feb 2006]

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