Lamb3 (NM_001100841) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217523 representing NM_001100841 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACGGCACTTTTCCTCTTGTGGCTTGCCTTGCCTGGCCTCCTGTGTGCCCAGCAACCCTGCTCCC GAGGGGCCTGTTATCCACCTGTTGGGGACTTGCTCATTGGAAGGACTCAGCTTCTTCGAGCCTCGTCTAC CTGTGGATTGACCAAGCCTGAGACCTATTGCACCCAATATGGACAGTGGCAGATGAAATGCTGCAAGTGT GACTCCAGGCTGCCTCGCAACTACAACAGTCACCGAGTGGAGAACGTTGTATCGTCTTCAGGCCCCATGC GCTGGTGGCAGTCCCAGAACGATGTGAGCCCTGTCTCTCTTCAACTGGACCTAGACAAGAGGATGCAGCT TCAGGACATAATGATGGATTTTAAGGGTCTCATGCCAGCTGGCATGTTGATTGAGCGCTCTTCTGACTTT GGCAAGACATGGAGGGTGTACCAGTACCTGGCGACAGACTGTGCCAGCACCTTTCCCCAGGTCCACCAGG GCCAGCCCAAGAACTGGCAGGACGTCCGGTGCCGGCCCTTGTCCCAGAGGCCTAATGGGCATCTGACTGG GGGAAAGGTCCAACTTAACCTTATGGATTTGGCATCAGCTATCCCTGCATCTCAAAGTAAAAAGATTCAA GAACTAGGAGACATCACGAACTTGAGAGTCAACTTCACCAAGCTGGCCCCAGTGCCCCAGAGGGGCTCCT ATCCACCCAGCGCCTACTTTGCAGTATCACAGCTACGTCTACAGGGGAGTTGCTTCTGTCATGGACATGC CGACCGCTGTGCCCCTAATCCTGGAGGCCCTACAGGTTCCACCACCGCTGTGCAGGTCAACGATGTCTGT GTCTGCCAGCACAACACAGCTGGCCCCAACTGTGACCGCTGCGCCCCCTTCTACAACAACCGGCCATGGA GACCTGCAGAGGGACAGGACATCCATGAGTGCCAAAGGTGTGACTGCAATGGGCACTCAGAGACCTGCCA CTTCGACCCAGCTGTGTTTGCTGCTAGCCAAGGGACAAGTGGAGGCGTGTGTGACAACTGCCAGGACCAC ACAGAGGGCAAGAACTGTGAGCGATGTCAGCTACACTATTTCCGGAATAGACGACCAGGCGCTCCCATTC ATGAGACCTGTATTCCCTGCGAGTGTGATCCAGACGGGGCAGTCCCAGGAGCTCCCTGTGACCGACTGAC GGGCCAGTGCGTGTGCAAAGACCACGTGCAAGGGGAGCGCTGTGATCTTTGCAAGCCTGGTTTCACAGGG CTCACCTTCGCCAACCCACAGGGCTGCCATCCCTGTGACTGCAGCATCTTAGGTACCCGGCAAGATATGC CTTGTGAGGAGGAAACTGGACGCTGCTTGTGTCTTCCCAATGTAGTGGGCCCCAAATGTGACCAGTGCGC CCCTTCCCACTGGAAGCTGGCCAGCGGCCGGGGCTGTGAGCCCTGTGCCTGTGACCCACGCAACTCCCTC AGCTCCCAGTGCAACCAGTTTACAGGGCAGTGTCCTTGTCGGGAAGGGTTTGGTGGCCTCACATGTAGTT CTGCAGCCATTCGTCAGTGTCCCGACCGGACCCATGGAGACGTGGCCACAGGGTGCCGAGCCTGTGACTG TGACTTCAGAGGAACCGAGGGTCCTGGCTGTGACAAAACCTCAGGCCGTTGCCTCTGCCGCCCCGGCTTG ACAGGGCCCCGCTGCGACCAGTGCCAACGAGGCTACTGCGACCGCTACCCAGTGTGTGTGGCCTGCCACT CTTGCTTCCAGGCCTATGACACAGACCTCCAGGAGCAGGCTTGGCGCCTTGGCAGTCTCCGTAATACCAC TGAAGGCCTGGGCACAGGGACAGGTCTGGAGGACCGTGGCCTGGCTTCTCGGTTGCTAGATGCCAAGAGC AAAATCGAGCAGATCAGACAGATTCTGGGTGGCACCTCTGTCACAGAGCGGGATGTGGCTCAAGTGGCCA ATGCCATCCTGTCTATCAGGAGGACTCTTCAGGGCCTGCCCCTGGACCTACCCTTAGAGGAGGAGATGGA ATCCTTCTCTGGAGACCTGGGGAATCTAGACAGAAGCTTCAATCGCCTCCTCCTTATGTACCGGAGCAAG AAGGAGCAGTTTGAGAAGCTAAGCAGTGTGGACCCTTCAGGAGCCTTCCGCATGCTGACTATGGCTTATG AGCAGTCCTCCCGGGCTGCACAGCAAGTATCCGACAGTTCCAGCCTGCTGAGCCAGCTGAGGGACAGTCG CAGAGAGGTAGAAGGTCTGGAGAGACAGACTGGAGAGGGAGGAGCTGGAGGAGCTCAGCTCATGGCCCTG CGCCTAGAGATGGCTTCACTGCCTGACTTGACACCCACCATCAACAAGCTCTGTGGTGGCTCTAGGCAGA CAGCCTGCACCCCAGGAGACTGCCCTGGATTGCTGTGTCCCCAGGACAATGGTACAGCCTGTGGCTCTCA CTGCAGGGGAGCCCTGCCCAGAGCCAGAGGGGCCTTACACATGGCAGGGCAGGTAGCTGAGCAGCTACGA AGCTTCAATACCCAGCTCCAGCAGACCAGGCAAATGATCAGGGCAGCTGAGGAGGCAGCATCAAAGGTCC AATCAGATGCCCAGCGCCTTGAGACTCAGGTGAGTACCAGCCGCTTACAGATGGAGGAAGATGTCCGGCG CACACGGCTTCTCATCCAGCAGGTCCGGGGCTTCCTCACAGACCCTCACACAGATGCAGCCACCATCCAA CAGGTGAGCGAGGCAGTGCTGGCTCTCTGGCTGCCCACAGACTCAGCTACAGTGCTACGCAAGATGAAAG AGATCCAGGACATTGCTGCCAGGCTCCCTAACGTGGACTTAGTGCTATCCCAGACTAAGCAGGACATTGC ACGGGCCCGCAGGCTCCAGGCTGAGGCCGAGAAGGCCAGAAGCCGAGCCCACGTTGTGGAAGGGCAGGTG GATGACGTGGTCGGGAACCTCAGGCAGGGCACCGTGGCTCTGCAGGAGGCTCAGGACACTATGCAGGGCA CTGGCCGCTCTCTTCGGCTCATCCAGGAAAGGGTTACTGAGGTTCAGCAGGTCCTGGTACCAGCTGAAAG GCTGGTGAAAGGCATGAAGGAACAGATGAGTGGATTCTGGGCACGGGTGAAGGAGCTCCGCCATCAGGCC CAGGAAGAGCAGGCACAGGCAATGAAGGCCCAGCAGCTTGCAGAGGGTGCCAGCAATCAGGCCATGAATG CTCAGGAGGGCTTTGAGAGACTTAAGCAAAGGTATACAGAGTTGAAAGACCGGCTGGGTCAGAGTTCTAT GCTGGGTGAGCAAGGCAATCGGATCTTGAGCATCAAGGCGGAGGCAGAGGAGCTCTTTGGGGAAACCATG GAAATGATGGATAAAATGAAAGACATGGAGTCAGAGCTGCTTCGAGGGAGTCAGGCCATCATGCTTCGTT CAGCAGACCTGTCGGGGCTGGAGAAGCGCGTGGAGCAGATCCGCAGTTACATCAACGGGCGTGTGCTGTA CTATGCCACCTGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217523 representing NM_001100841 Red=Cloning site Green=Tags(s) MMTALFLLWLALPGLLCAQQPCSRGACYPPVGDLLIGRTQLLRASSTCGLTKPETYCTQYGQWQMKCCKC DSRLPRNYNSHRVENVVSSSGPMRWWQSQNDVSPVSLQLDLDKRMQLQDIMMDFKGLMPAGMLIERSSDF GKTWRVYQYLATDCASTFPQVHQGQPKNWQDVRCRPLSQRPNGHLTGGKVQLNLMDLASAIPASQSKKIQ ELGDITNLRVNFTKLAPVPQRGSYPPSAYFAVSQLRLQGSCFCHGHADRCAPNPGGPTGSTTAVQVNDVC VCQHNTAGPNCDRCAPFYNNRPWRPAEGQDIHECQRCDCNGHSETCHFDPAVFAASQGTSGGVCDNCQDH TEGKNCERCQLHYFRNRRPGAPIHETCIPCECDPDGAVPGAPCDRLTGQCVCKDHVQGERCDLCKPGFTG LTFANPQGCHPCDCSILGTRQDMPCEEETGRCLCLPNVVGPKCDQCAPSHWKLASGRGCEPCACDPRNSL SSQCNQFTGQCPCREGFGGLTCSSAAIRQCPDRTHGDVATGCRACDCDFRGTEGPGCDKTSGRCLCRPGL TGPRCDQCQRGYCDRYPVCVACHSCFQAYDTDLQEQAWRLGSLRNTTEGLGTGTGLEDRGLASRLLDAKS KIEQIRQILGGTSVTERDVAQVANAILSIRRTLQGLPLDLPLEEEMESFSGDLGNLDRSFNRLLLMYRSK KEQFEKLSSVDPSGAFRMLTMAYEQSSRAAQQVSDSSSLLSQLRDSRREVEGLERQTGEGGAGGAQLMAL RLEMASLPDLTPTINKLCGGSRQTACTPGDCPGLLCPQDNGTACGSHCRGALPRARGALHMAGQVAEQLR SFNTQLQQTRQMIRAAEEAASKVQSDAQRLETQVSTSRLQMEEDVRRTRLLIQQVRGFLTDPHTDAATIQ QVSEAVLALWLPTDSATVLRKMKEIQDIAARLPNVDLVLSQTKQDIARARRLQAEAEKARSRAHVVEGQV DDVVGNLRQGTVALQEAQDTMQGTGRSLRLIQERVTEVQQVLVPAERLVKGMKEQMSGFWARVKELRHQA QEEQAQAMKAQQLAEGASNQAMNAQEGFERLKQRYTELKDRLGQSSMLGEQGNRILSIKAEAEELFGETM EMMDKMKDMESELLRGSQAIMLRSADLSGLEKRVEQIRSYINGRVLYYATCK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100841
ORF Size	3516 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100841.1, NP_001094311.1
RefSeq Size	4078 bp
RefSeq ORF	3519 bp
Locus ID	305078
MW	129.1 kDa

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