Lamb3 (NM_001100841) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217523 representing NM_001100841 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACGGCACTTTTCCTCTTGTGGCTTGCCTTGCCTGGCCTCCTGTGTGCCCAGCAACCCTGCTCCC GAGGGGCCTGTTATCCACCTGTTGGGGACTTGCTCATTGGAAGGACTCAGCTTCTTCGAGCCTCGTCTAC CTGTGGATTGACCAAGCCTGAGACCTATTGCACCCAATATGGACAGTGGCAGATGAAATGCTGCAAGTGT GACTCCAGGCTGCCTCGCAACTACAACAGTCACCGAGTGGAGAACGTTGTATCGTCTTCAGGCCCCATGC GCTGGTGGCAGTCCCAGAACGATGTGAGCCCTGTCTCTCTTCAACTGGACCTAGACAAGAGGATGCAGCT TCAGGACATAATGATGGATTTTAAGGGTCTCATGCCAGCTGGCATGTTGATTGAGCGCTCTTCTGACTTT GGCAAGACATGGAGGGTGTACCAGTACCTGGCGACAGACTGTGCCAGCACCTTTCCCCAGGTCCACCAGG GCCAGCCCAAGAACTGGCAGGACGTCCGGTGCCGGCCCTTGTCCCAGAGGCCTAATGGGCATCTGACTGG GGGAAAGGTCCAACTTAACCTTATGGATTTGGCATCAGCTATCCCTGCATCTCAAAGTAAAAAGATTCAA GAACTAGGAGACATCACGAACTTGAGAGTCAACTTCACCAAGCTGGCCCCAGTGCCCCAGAGGGGCTCCT ATCCACCCAGCGCCTACTTTGCAGTATCACAGCTACGTCTACAGGGGAGTTGCTTCTGTCATGGACATGC CGACCGCTGTGCCCCTAATCCTGGAGGCCCTACAGGTTCCACCACCGCTGTGCAGGTCAACGATGTCTGT GTCTGCCAGCACAACACAGCTGGCCCCAACTGTGACCGCTGCGCCCCCTTCTACAACAACCGGCCATGGA GACCTGCAGAGGGACAGGACATCCATGAGTGCCAAAGGTGTGACTGCAATGGGCACTCAGAGACCTGCCA CTTCGACCCAGCTGTGTTTGCTGCTAGCCAAGGGACAAGTGGAGGCGTGTGTGACAACTGCCAGGACCAC ACAGAGGGCAAGAACTGTGAGCGATGTCAGCTACACTATTTCCGGAATAGACGACCAGGCGCTCCCATTC ATGAGACCTGTATTCCCTGCGAGTGTGATCCAGACGGGGCAGTCCCAGGAGCTCCCTGTGACCGACTGAC GGGCCAGTGCGTGTGCAAAGACCACGTGCAAGGGGAGCGCTGTGATCTTTGCAAGCCTGGTTTCACAGGG CTCACCTTCGCCAACCCACAGGGCTGCCATCCCTGTGACTGCAGCATCTTAGGTACCCGGCAAGATATGC CTTGTGAGGAGGAAACTGGACGCTGCTTGTGTCTTCCCAATGTAGTGGGCCCCAAATGTGACCAGTGCGC CCCTTCCCACTGGAAGCTGGCCAGCGGCCGGGGCTGTGAGCCCTGTGCCTGTGACCCACGCAACTCCCTC AGCTCCCAGTGCAACCAGTTTACAGGGCAGTGTCCTTGTCGGGAAGGGTTTGGTGGCCTCACATGTAGTT CTGCAGCCATTCGTCAGTGTCCCGACCGGACCCATGGAGACGTGGCCACAGGGTGCCGAGCCTGTGACTG TGACTTCAGAGGAACCGAGGGTCCTGGCTGTGACAAAACCTCAGGCCGTTGCCTCTGCCGCCCCGGCTTG ACAGGGCCCCGCTGCGACCAGTGCCAACGAGGCTACTGCGACCGCTACCCAGTGTGTGTGGCCTGCCACT CTTGCTTCCAGGCCTATGACACAGACCTCCAGGAGCAGGCTTGGCGCCTTGGCAGTCTCCGTAATACCAC TGAAGGCCTGGGCACAGGGACAGGTCTGGAGGACCGTGGCCTGGCTTCTCGGTTGCTAGATGCCAAGAGC AAAATCGAGCAGATCAGACAGATTCTGGGTGGCACCTCTGTCACAGAGCGGGATGTGGCTCAAGTGGCCA ATGCCATCCTGTCTATCAGGAGGACTCTTCAGGGCCTGCCCCTGGACCTACCCTTAGAGGAGGAGATGGA ATCCTTCTCTGGAGACCTGGGGAATCTAGACAGAAGCTTCAATCGCCTCCTCCTTATGTACCGGAGCAAG AAGGAGCAGTTTGAGAAGCTAAGCAGTGTGGACCCTTCAGGAGCCTTCCGCATGCTGACTATGGCTTATG AGCAGTCCTCCCGGGCTGCACAGCAAGTATCCGACAGTTCCAGCCTGCTGAGCCAGCTGAGGGACAGTCG CAGAGAGGTAGAAGGTCTGGAGAGACAGACTGGAGAGGGAGGAGCTGGAGGAGCTCAGCTCATGGCCCTG CGCCTAGAGATGGCTTCACTGCCTGACTTGACACCCACCATCAACAAGCTCTGTGGTGGCTCTAGGCAGA CAGCCTGCACCCCAGGAGACTGCCCTGGATTGCTGTGTCCCCAGGACAATGGTACAGCCTGTGGCTCTCA CTGCAGGGGAGCCCTGCCCAGAGCCAGAGGGGCCTTACACATGGCAGGGCAGGTAGCTGAGCAGCTACGA AGCTTCAATACCCAGCTCCAGCAGACCAGGCAAATGATCAGGGCAGCTGAGGAGGCAGCATCAAAGGTCC AATCAGATGCCCAGCGCCTTGAGACTCAGGTGAGTACCAGCCGCTTACAGATGGAGGAAGATGTCCGGCG CACACGGCTTCTCATCCAGCAGGTCCGGGGCTTCCTCACAGACCCTCACACAGATGCAGCCACCATCCAA CAGGTGAGCGAGGCAGTGCTGGCTCTCTGGCTGCCCACAGACTCAGCTACAGTGCTACGCAAGATGAAAG AGATCCAGGACATTGCTGCCAGGCTCCCTAACGTGGACTTAGTGCTATCCCAGACTAAGCAGGACATTGC ACGGGCCCGCAGGCTCCAGGCTGAGGCCGAGAAGGCCAGAAGCCGAGCCCACGTTGTGGAAGGGCAGGTG GATGACGTGGTCGGGAACCTCAGGCAGGGCACCGTGGCTCTGCAGGAGGCTCAGGACACTATGCAGGGCA CTGGCCGCTCTCTTCGGCTCATCCAGGAAAGGGTTACTGAGGTTCAGCAGGTCCTGGTACCAGCTGAAAG GCTGGTGAAAGGCATGAAGGAACAGATGAGTGGATTCTGGGCACGGGTGAAGGAGCTCCGCCATCAGGCC CAGGAAGAGCAGGCACAGGCAATGAAGGCCCAGCAGCTTGCAGAGGGTGCCAGCAATCAGGCCATGAATG CTCAGGAGGGCTTTGAGAGACTTAAGCAAAGGTATACAGAGTTGAAAGACCGGCTGGGTCAGAGTTCTAT GCTGGGTGAGCAAGGCAATCGGATCTTGAGCATCAAGGCGGAGGCAGAGGAGCTCTTTGGGGAAACCATG GAAATGATGGATAAAATGAAAGACATGGAGTCAGAGCTGCTTCGAGGGAGTCAGGCCATCATGCTTCGTT CAGCAGACCTGTCGGGGCTGGAGAAGCGCGTGGAGCAGATCCGCAGTTACATCAACGGGCGTGTGCTGTA CTATGCCACCTGCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100841
Insert Size	3519 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100841.1, NP_001094311.1
RefSeq Size	4078 bp
RefSeq ORF	3519 bp
Locus ID	305078

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