Smg7 (NM_001191549) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1310761
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217509 representing NM_001191549 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACCGAAAACTTGAAATCAGAGGAGCATCTGAAATCAAGTAATATTAGGCAGGCAGAAGTCCTGA AGGCTGAAATGACAGATTCTAAGCTGGGTCCAGCTGAGGTCTGGACATCCAGGCAAGCTCTGCAGGACTT GTACCAGAAAATGCTGGTTACTGATCTGGAGTATGCTTTAGACAAGAAGGTAGAGCAGGACCTCTGGAAT CATGCCTTTAAGAATCAGATCACAACACTACAAGGCCAAGCCAAGAATCGAGCAAACCCAAATCGGAGTG AAGTCCAGGCAAACCTTTCTCTGTTCCTAGAGGCAGCTAGCGGCTTCTATACTCAGTTATTACAGGAACT GTGTACGGTGTTTAATGTAGATTTGCCATGCCGTGTGAAGTCTTCCCAGTTGGGAATTATTAGCAATAAA CAGACGCACACCAGCGCCATAGTGAAGCCACAGTCTAGCTCCTGTTCCTACATTTGCCAGCACTGCCTCG TCCACCTTGGAGACATTGCTCGATACAGAAACCAGACTAGCCAGGCAGAGTCCTACTATAGGCATGCAGC TCAGCTTGTCCCCTCCAATGGTCAGCCTTACAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCAC CTGACCACAATTTTCTACTACTGCAGAAGCATTGCTGTGAAATTCCCTTTCCCAGCTGCCTCCACTAACC TACAAAAAGCACTTTCTAAAGCACTGGAAAGCCGGGATGAGTTGAAAACCAAGTGGGGTGTTTCTGACTT CATCAAGGCCTTTATTAAATTCCACGGTCATGTATACCTGAGTAAGAGTTTGGAAAAGCTGAGCCCTCTT CGAGAGAAGTTAGAAGAACAGTTTAAGAGACTGCTGTTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTTC ATGTCACTGTCATTAACCTGTTTCAACTTCATCATCTTCGTGACTTTAGCAATGAAACAGAGCAGCACAG TTACAGCCAAGATGAACAGCTGTGTTGGACACAGTTGCTGGCCCTCTTCATGTCTTTTCTTGGCATCCTG TGCAAATGTCCTCTCCAGAATGATTCTCAGGAGTCCTACAATGCCTATCCCCTTCCTGCAGTCAAGGTCT CCATGGACTGGCTAAGACTCCGACCTAGAGTCTTTCAAGAAGCTGTAGTGGATGAAAGACAGTACATCTG GCCCTGGCTAATTTCTCTTCTCAATAGTTTTCATCCCCGTGAAGATGATCTCTCAAGTACTAATGCCACA CCACTTCCAGAGGAGTTTGAACTACAAGGGTTCCTGGCTTTGAGACCTTCTTTCAGGAACTTGGATTTTT CCAAAGGCCATCAGGGTATTACAGGAGACAAAGAGGGTCAACAACGGCGAATACGGCAGCAGCGTTTGAT CTCCATAGGGAAGTGGATTGCTGACAACCAGCCACGGCTGATTCAGTGTGAAAATGAGGTAGGGAAATTG TTATTTATCACAGAAATCCCAGAATTAATACTAGAAGACCCCAGTGAAGCCAAAGAGAACCTCATCCTAC AAGAAACATCTGCGATAGAGTCACACGCTTCGGACGGGAGCCCAGGACTGAAGTCAGTGCTGTCTACAGG CCGAAGTCCAAGCACCAGCTGTGACTCAGGAGAGAAGCCAGTGGTCACCTTCAAAGAGAACATTAAGCCA CGAGAAGTGAACAGAGACCAAGGAAGAAGCTTTCCTCCCAAAGAGGTAAAATCCCAGACAGAACTAAGAA AGACCCCAGTGTCCGAAGCCAGGAAAACTCCTGTCACTCAAACTCCAAGTCAAACAAATAATTCTCAGTT CATCCCCATCCATCACCCTGGAGCCTTCCCTCCTCTTCCCAGCCGACCGGGGTTCCCGCCCCCAACATAT GTTATCCCCCCTCCTGTGGCATTTTCTATGGGCTCAGGATACACCTTCCCAGCTGGTGTTTCTGTCCCAG GAACCTTTCTTCAGTCTACAGCTCACTCTCCAGCAGGAAACCAGGTGCAAGCTGGGAAACAGTCCCACAT TCCTTACAGCCAGCAACGGCCCTCCGGACCAGGGCCAATGAACCAGGGACCTCAACAATCACAGCCACCT TCCCAGCCACCCCTTACATCTTTACCAGCTCAGCCAACAGCACAGTCTACAAGCCAGTTGCAGGTTCAAG CTCTAGCTCAGCAACAGCAGTCCCCTACAAAAGTCATACCAGCCTTGGGGAAAAGCCCGCCTCACCACTC TGGATTCCAGCAGTATCAACAGGCAGATGCCTCCAAACAGCTGTGGAATCCCCCTCAGGTTCAAAGCCCA CTAGGGAAAATTATGCCTGTGAAACAGCCCTACTACCTTCAGACCCAAGACCCTATAAAACTGTTTGAGC CGTCATTGCAGCCTCCTGTAATACAGCAACAGCCTCTAGAGAAAAAAATGAAGCCTTTCCCCATGGAGCC ATATAACCATAATCCCTCAGAAGTCAAGGTCCCAGAGTTCTACTGGGATTCTTCCTACAGCATGGCTGAT AACAGAGCAGTAATGGCTCAGCAACCAAATATGGACCGCAGGAGCAAACGGTCACCCGGAGTCTTCCGTC CAGAGCAGGATCCTGTGCCCAGGATGCCGTTTGAGGACCCCAAGAGCTCCCCTCTGCTTCCTCCGGACCT GTTAAAGAGTCTGGCTGCCTTGGAGGAAGAGGAAGAGCTGATCTTTTCTAACCCTCCTGATCTTTACCCA GCTCTGCTGGGTCCTCTCGCCTCTCTTCCTGGACGAAGCCTCTTTAAATCCTTACTGGAGAAGCCTTCCG AGCTCATGTCACATTCATCTTCTTTCCTGTCCCTCACTGGGTTCTCTGTCAATCAGGAAAGATATCCAAA CAGCAGTGTGTTCAATGAAGTGTATGGGAAGAACCTGACAGCCAGCTCCAAGGCAGAGCTGAGCCCCTCG GTTGCCTCCTCCCAGGAAACATCACTGTACTCCCTCTTCGAAGGGACCCCTTGGTCTCCATCACTTCCTG CCAGTTCAGATCATTCAACACCAGCCAGCCAGTCTCCTCATTCCTCCAACCCAAGCAGCCTGCCCAGTTC TCCTCCAACACATAACCATAATTCTGCTCCATTCTCTAATTTTGGACCCATTGGGACTCCAGATAACAGG GATAGGCGGCCTGCAGATAGGTGGAAAACTGATAAACCAGCCATGGGTGGTTTCGGCGTTGATTACCTCT CAGCAACGTCATCATCTGAGAACAGTTGGCATCAGGCTAGCACTCCAAGTGGCACCTGGACAGGCCACGG CCCCTCCATGGAGGATTCCTCCGCTGTCCTCATGGAAAGCCTAAAGTCTATCTGGTCCAGTTCCATGATG CATCCTGGACCCTCCGCTCTCGAGCAGTTGTTAATGCAGCAGAAGCAGAAACAGCAGCGGGGACAAGGTG CCATGAACCCTCCACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217509 representing NM_001191549 Red=Cloning site Green=Tags(s) MRTENLKSEEHLKSSNIRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWN HAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNK QTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDH LTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPL REKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLALFMSFLGIL CKCPLQNDSQESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPREDDLSSTNAT PLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKL LFITEIPELILEDPSEAKENLILQETSAIESHASDGSPGLKSVLSTGRSPSTSCDSGEKPVVTFKENIKP REVNRDQGRSFPPKEVKSQTELRKTPVSEARKTPVTQTPSQTNNSQFIPIHHPGAFPPLPSRPGFPPPTY VIPPPVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPP SQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQQYQQADASKQLWNPPQVQSP LGKIMPVKQPYYLQTQDPIKLFEPSLQPPVIQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMAD NRAVMAQQPNMDRRSKRSPGVFRPEQDPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYP ALLGPLASLPGRSLFKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSVFNEVYGKNLTASSKAELSPS VASSQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNFGPIGTPDNR DRRPADRWKTDKPAMGGFGVDYLSATSSSENSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMM HPGPSALEQLLMQQKQKQQRGQGAMNPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191549
ORF Size	3447 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191549.1, NP_001178478.1
RefSeq Size	5719 bp
RefSeq ORF	3450 bp
Locus ID	360855
MW	128.1 kDa

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