Smg7 (NM_001191549) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1310761
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217509 representing NM_001191549 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGACCGAAAACTTGAAATCAGAGGAGCATCTGAAATCAAGTAATATTAGGCAGGCAGAAGTCCTGA AGGCTGAAATGACAGATTCTAAGCTGGGTCCAGCTGAGGTCTGGACATCCAGGCAAGCTCTGCAGGACTT GTACCAGAAAATGCTGGTTACTGATCTGGAGTATGCTTTAGACAAGAAGGTAGAGCAGGACCTCTGGAAT CATGCCTTTAAGAATCAGATCACAACACTACAAGGCCAAGCCAAGAATCGAGCAAACCCAAATCGGAGTG AAGTCCAGGCAAACCTTTCTCTGTTCCTAGAGGCAGCTAGCGGCTTCTATACTCAGTTATTACAGGAACT GTGTACGGTGTTTAATGTAGATTTGCCATGCCGTGTGAAGTCTTCCCAGTTGGGAATTATTAGCAATAAA CAGACGCACACCAGCGCCATAGTGAAGCCACAGTCTAGCTCCTGTTCCTACATTTGCCAGCACTGCCTCG TCCACCTTGGAGACATTGCTCGATACAGAAACCAGACTAGCCAGGCAGAGTCCTACTATAGGCATGCAGC TCAGCTTGTCCCCTCCAATGGTCAGCCTTACAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCAC CTGACCACAATTTTCTACTACTGCAGAAGCATTGCTGTGAAATTCCCTTTCCCAGCTGCCTCCACTAACC TACAAAAAGCACTTTCTAAAGCACTGGAAAGCCGGGATGAGTTGAAAACCAAGTGGGGTGTTTCTGACTT CATCAAGGCCTTTATTAAATTCCACGGTCATGTATACCTGAGTAAGAGTTTGGAAAAGCTGAGCCCTCTT CGAGAGAAGTTAGAAGAACAGTTTAAGAGACTGCTGTTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTTC ATGTCACTGTCATTAACCTGTTTCAACTTCATCATCTTCGTGACTTTAGCAATGAAACAGAGCAGCACAG TTACAGCCAAGATGAACAGCTGTGTTGGACACAGTTGCTGGCCCTCTTCATGTCTTTTCTTGGCATCCTG TGCAAATGTCCTCTCCAGAATGATTCTCAGGAGTCCTACAATGCCTATCCCCTTCCTGCAGTCAAGGTCT CCATGGACTGGCTAAGACTCCGACCTAGAGTCTTTCAAGAAGCTGTAGTGGATGAAAGACAGTACATCTG GCCCTGGCTAATTTCTCTTCTCAATAGTTTTCATCCCCGTGAAGATGATCTCTCAAGTACTAATGCCACA CCACTTCCAGAGGAGTTTGAACTACAAGGGTTCCTGGCTTTGAGACCTTCTTTCAGGAACTTGGATTTTT CCAAAGGCCATCAGGGTATTACAGGAGACAAAGAGGGTCAACAACGGCGAATACGGCAGCAGCGTTTGAT CTCCATAGGGAAGTGGATTGCTGACAACCAGCCACGGCTGATTCAGTGTGAAAATGAGGTAGGGAAATTG TTATTTATCACAGAAATCCCAGAATTAATACTAGAAGACCCCAGTGAAGCCAAAGAGAACCTCATCCTAC AAGAAACATCTGCGATAGAGTCACACGCTTCGGACGGGAGCCCAGGACTGAAGTCAGTGCTGTCTACAGG CCGAAGTCCAAGCACCAGCTGTGACTCAGGAGAGAAGCCAGTGGTCACCTTCAAAGAGAACATTAAGCCA CGAGAAGTGAACAGAGACCAAGGAAGAAGCTTTCCTCCCAAAGAGGTAAAATCCCAGACAGAACTAAGAA AGACCCCAGTGTCCGAAGCCAGGAAAACTCCTGTCACTCAAACTCCAAGTCAAACAAATAATTCTCAGTT CATCCCCATCCATCACCCTGGAGCCTTCCCTCCTCTTCCCAGCCGACCGGGGTTCCCGCCCCCAACATAT GTTATCCCCCCTCCTGTGGCATTTTCTATGGGCTCAGGATACACCTTCCCAGCTGGTGTTTCTGTCCCAG GAACCTTTCTTCAGTCTACAGCTCACTCTCCAGCAGGAAACCAGGTGCAAGCTGGGAAACAGTCCCACAT TCCTTACAGCCAGCAACGGCCCTCCGGACCAGGGCCAATGAACCAGGGACCTCAACAATCACAGCCACCT TCCCAGCCACCCCTTACATCTTTACCAGCTCAGCCAACAGCACAGTCTACAAGCCAGTTGCAGGTTCAAG CTCTAGCTCAGCAACAGCAGTCCCCTACAAAAGTCATACCAGCCTTGGGGAAAAGCCCGCCTCACCACTC TGGATTCCAGCAGTATCAACAGGCAGATGCCTCCAAACAGCTGTGGAATCCCCCTCAGGTTCAAAGCCCA CTAGGGAAAATTATGCCTGTGAAACAGCCCTACTACCTTCAGACCCAAGACCCTATAAAACTGTTTGAGC CGTCATTGCAGCCTCCTGTAATACAGCAACAGCCTCTAGAGAAAAAAATGAAGCCTTTCCCCATGGAGCC ATATAACCATAATCCCTCAGAAGTCAAGGTCCCAGAGTTCTACTGGGATTCTTCCTACAGCATGGCTGAT AACAGAGCAGTAATGGCTCAGCAACCAAATATGGACCGCAGGAGCAAACGGTCACCCGGAGTCTTCCGTC CAGAGCAGGATCCTGTGCCCAGGATGCCGTTTGAGGACCCCAAGAGCTCCCCTCTGCTTCCTCCGGACCT GTTAAAGAGTCTGGCTGCCTTGGAGGAAGAGGAAGAGCTGATCTTTTCTAACCCTCCTGATCTTTACCCA GCTCTGCTGGGTCCTCTCGCCTCTCTTCCTGGACGAAGCCTCTTTAAATCCTTACTGGAGAAGCCTTCCG AGCTCATGTCACATTCATCTTCTTTCCTGTCCCTCACTGGGTTCTCTGTCAATCAGGAAAGATATCCAAA CAGCAGTGTGTTCAATGAAGTGTATGGGAAGAACCTGACAGCCAGCTCCAAGGCAGAGCTGAGCCCCTCG GTTGCCTCCTCCCAGGAAACATCACTGTACTCCCTCTTCGAAGGGACCCCTTGGTCTCCATCACTTCCTG CCAGTTCAGATCATTCAACACCAGCCAGCCAGTCTCCTCATTCCTCCAACCCAAGCAGCCTGCCCAGTTC TCCTCCAACACATAACCATAATTCTGCTCCATTCTCTAATTTTGGACCCATTGGGACTCCAGATAACAGG GATAGGCGGCCTGCAGATAGGTGGAAAACTGATAAACCAGCCATGGGTGGTTTCGGCGTTGATTACCTCT CAGCAACGTCATCATCTGAGAACAGTTGGCATCAGGCTAGCACTCCAAGTGGCACCTGGACAGGCCACGG CCCCTCCATGGAGGATTCCTCCGCTGTCCTCATGGAAAGCCTAAAGTCTATCTGGTCCAGTTCCATGATG CATCCTGGACCCTCCGCTCTCGAGCAGTTGTTAATGCAGCAGAAGCAGAAACAGCAGCGGGGACAAGGTG CCATGAACCCTCCACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191549
Insert Size	3450 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191549.1, NP_001178478.1
RefSeq Size	5719 bp
RefSeq ORF	3450 bp
Locus ID	360855

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