Itga8 (NM_001173972) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1564327
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217446 representing NM_001173972 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGAGAACCCACCGGGGTCCCCCGGAGAACTGGGCACCTCCGTTCGCTTGTCTCTGTTGCGTCT CGGCCGTGCTGGGGATGCTGTGGTCCCCCGTGTCTCTGGCGTTCAACTTGGACGTAGACAAGCTCACGGT GTACAGTGGCCCCGAGGGCAGCTACTTCGGCTACTCGCTGGACTTCTACATACCTGATGCGCGCACAGCC AGTGTCTTGGTGGGGGCGCCCAAAGCCAACACTAGCCAGCCGGATATCGTAGAAGGGGGAGCTGTCTACT ACTGTCCCTGGCCTGCAGAGAGGTCTGCACAGTGCAAGCAGATACCCTTTGACACCACCAACAACAGGAA AATCAGAGTTAATGGAACCAAAGAGCCTATAGAATTTAAATCAAACCAATGGTTTGGAGCCACAGTGAGA GCTCACAAAGGAAAAGTTGTGGCTTGTGCCCCTTTATATCACTGGAGAACTCTGAAGCCTAATCCAGCAA AGGACCCGGTTGGCACATGCTATGTAGCAATTCAGAACTTCAGTGCTTACGCTGAGCACTCACCCTGTCG GAACAGCAACGTTGATCCTGAAGGCCAAGGTTACTGCCAAGCCGGCTTCAGTCTAGACTTCTATAAGAAT GGAGACCTTATAGTGGGAGGACCTGGAAGCTTTTACTGGCAAGGTCAGGTGATCACGGTCAGTGTAGCAG ACATCATTGCAAATTACTCATTCAAAGACATTCTTCGAAAACTGGCAGCAGAAAAGCAGACAGATGTGGC TCCAGCTTCCTATGATGACAGCTACCTTGGATATTCAGTTGCTGCTGGAGAATTCACTGGGGACTCTCTG CAAGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAACTTTGGATATGTCTCCATCATTAACTCCACAG ATATGACCTTCATTCAGAATTTTACTGGTGAGCAGATGGCATCTTATTTCGGATATACTGTTGTGGTATC AGATGTTAACAATGATGGCATGGATGACATATTGGTCGGGGCACCTCTCTTTATGGAGCGAGAATTTGAA AGTAACCCTAGAGAAGTGGGTCAGGTCTACCTGTACCTACAAGTGAGCGCCCTCCTCTTCCAAGACCCAC AGGTCCTTACGGGCACAGAGACTTTTGGGAGATTCGGTAGTTCTGTGGCCCACTTGGGGGACCTGAACCA AGATGGATACAATGATATTGCCATCGGTGTGCCCTTTGCAGGCAAGGATCAAAGAGGTAAAGTCCTCATT TACAACGGAAACGCCAGAGGCTTGCACAGCAAGCCTTCCCAAGTTCTGCAAGGAATATGGGGGTCACAGA CCATCCCTTCTGGATTCGGCTTTTCTCTAAGAGGAGACGCAGACATAGACAAGAATGATTACCCAGATTT ACTTGTCGGGGCATTTGGAGAAGGGAAAGTGGCCGTGTACAGAGCAAGGCCAGTTGTAACAGTGGATGCC CAGCTTCTGCTGCACCCAATGATTATCAACCTTGAAAATAAAACTTGCCAGATTCCAGAATTCCCGACTC CTGTGGCCTGCTTTTCTTTAAGAGTCTGTGCATCTATAGCAGGCCAGAGCATTTCAAATACAGTAGCCCT GATGGCCGAGGTGCAGTTAGATTTCCTGAAGCAAAAAGGAGCCATCAAACGGACCCTCTTCCTCCACAAC CACCAGTCCCACCTCATCTTCCCCTTCGTGATGAGGCAGCAAAAATCCCTCCACTGCCAGGATTTTATGG TTTACCTTCGAGATGAAACTGAATTCCGAGATAAACTGTCTCCAATCAACGTTAGCCTGAACTACAGTTT GGATGATTCCACCTTTGAAGATGGCCTGGAAGTGAAGCCGATTTTGAACCACTACAGGGAGAATGTCGTT ACTGAGCAGGCTCACATCCTGGTGGACTGTGGAGAAGACAACCTCTGTGTTCCTGACTTGAGGCTGTCAG CTAGACCAGATAAGCAGGAGATAATTATTGGGGATGAAAATCACCTAATGCTCATAATCAATGCAAGGAA TGAAGGAGAAGGAGCTTACGAAGCTGAACTCTTCGTCATGATTCCCGAAGAAGCAGATTACGTTGGGATC GAGCGCAACAACAAGGGACTGAGGCTCCTGAGTTGTGAGTACAAGATGGAAAACGTAACCAGGATGGTGG TGTGTGACCTTGGGAACCCGATGGTGACTGGAACGAATTTTTCTCTGGGCCTCCGATTTGCTGTTCCTCG TCTTGAGAAAACAAACATGAGTATTAACTTCGATCTCCAAATCAGAAGCTCCAACAAGGACAATCCTGAC AGCAATTTCGTGAGCGTGCAAATCAACGTCACTGCCGTGGCTCAGGTGGAAATCAGAGGAGTGTCCCACC CTCCCCAAATTGTTCTGCCCATCCACAACTGGGAGCCAGCAGAGGAGCCCCACAAAGAGGAGGAAGTCGG TCCCCTGGTTGAACATATTTATGAGCTGCACAATATTGGACCCAGCACCATCAGTGACAGCATTCTCGAA GTGGGCTGGCCATTCTCTGCACGGGAGGAGTTCCTCCTCTATGTCTTTCATCTTCAAACTCTGGGACCTC TACAATGTCAAACAAATCCTGAGATCAACCCGCAGGATATAAAGCCTGCTGCCTCCCCAGAGGATACCCC TGAGCTCAGCGCCTTCTTAAGAAATGCTACAATTCCCCATCTTGTCAGGAAGAGGGATGTGCCAGTGGTC CAGCCCCACAGACAGAGCCCGGCAAAAATACTGAACTGTACCAACATTGACTGCTTGCAAATCTCGTGTG CAGTGGGTCGCCTGGGAGGAGGAGAAAGTGCAGTTCTGAAGGTCAGATCGAGATTGTGGGCGCACACATT CCTCCAGAGAAAGAACGACCCTTATGCTCTTGCATCCCTGGTGTCCTTTGAAGTCAAGAAGATACCTTAT AAAGAGCAGCCAGCAAAACTGCCAGCCGGGAGCACAGCGATTAAGACATCGGTTATTTGGGCCACTCCAA ATGTCTCCTTCTCAATTCCACTGTGGGTCATAATACTAGCGATCCTTCTTGGCTTGCTGGTTCTGGCCAT CTTAACTTTAGCTTTATGGAAGTGTGGATTCTTTGATAGAGCAAGACCTCCTCAGGATGAAATGACAGAC AGGGAACAACTGACCAGTGACAAGACCCCGGAGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217446 representing NM_001173972 Red=Cloning site Green=Tags(s) MSARTHRGPPENWAPPFACLCCVSAVLGMLWSPVSLAFNLDVDKLTVYSGPEGSYFGYSLDFYIPDARTA SVLVGAPKANTSQPDIVEGGAVYYCPWPAERSAQCKQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVR AHKGKVVACAPLYHWRTLKPNPAKDPVGTCYVAIQNFSAYAEHSPCRNSNVDPEGQGYCQAGFSLDFYKN GDLIVGGPGSFYWQGQVITVSVADIIANYSFKDILRKLAAEKQTDVAPASYDDSYLGYSVAAGEFTGDSL QELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNNDGMDDILVGAPLFMEREFE SNPREVGQVYLYLQVSALLFQDPQVLTGTETFGRFGSSVAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLI YNGNARGLHSKPSQVLQGIWGSQTIPSGFGFSLRGDADIDKNDYPDLLVGAFGEGKVAVYRARPVVTVDA QLLLHPMIINLENKTCQIPEFPTPVACFSLRVCASIAGQSISNTVALMAEVQLDFLKQKGAIKRTLFLHN HQSHLIFPFVMRQQKSLHCQDFMVYLRDETEFRDKLSPINVSLNYSLDDSTFEDGLEVKPILNHYRENVV TEQAHILVDCGEDNLCVPDLRLSARPDKQEIIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVGI ERNNKGLRLLSCEYKMENVTRMVVCDLGNPMVTGTNFSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPD SNFVSVQINVTAVAQVEIRGVSHPPQIVLPIHNWEPAEEPHKEEEVGPLVEHIYELHNIGPSTISDSILE VGWPFSAREEFLLYVFHLQTLGPLQCQTNPEINPQDIKPAASPEDTPELSAFLRNATIPHLVRKRDVPVV QPHRQSPAKILNCTNIDCLQISCAVGRLGGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKIPY KEQPAKLPAGSTAIKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQDEMTD REQLTSDKTPEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001173972
ORF Size	3186 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001173972.1, NP_001167443.1
RefSeq Size	5896 bp
RefSeq ORF	3189 bp
Locus ID	364786
MW	117.6 kDa
Gene Summary	acts as a receptor for fibronectin; may be a receptor of matrix ligands and function in glomerular capillary tuft development and physiology [RGD, Feb 2006]

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