Itga8 (NM_001173972) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1564327
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217446 representing NM_001173972 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGAGAACCCACCGGGGTCCCCCGGAGAACTGGGCACCTCCGTTCGCTTGTCTCTGTTGCGTCT CGGCCGTGCTGGGGATGCTGTGGTCCCCCGTGTCTCTGGCGTTCAACTTGGACGTAGACAAGCTCACGGT GTACAGTGGCCCCGAGGGCAGCTACTTCGGCTACTCGCTGGACTTCTACATACCTGATGCGCGCACAGCC AGTGTCTTGGTGGGGGCGCCCAAAGCCAACACTAGCCAGCCGGATATCGTAGAAGGGGGAGCTGTCTACT ACTGTCCCTGGCCTGCAGAGAGGTCTGCACAGTGCAAGCAGATACCCTTTGACACCACCAACAACAGGAA AATCAGAGTTAATGGAACCAAAGAGCCTATAGAATTTAAATCAAACCAATGGTTTGGAGCCACAGTGAGA GCTCACAAAGGAAAAGTTGTGGCTTGTGCCCCTTTATATCACTGGAGAACTCTGAAGCCTAATCCAGCAA AGGACCCGGTTGGCACATGCTATGTAGCAATTCAGAACTTCAGTGCTTACGCTGAGCACTCACCCTGTCG GAACAGCAACGTTGATCCTGAAGGCCAAGGTTACTGCCAAGCCGGCTTCAGTCTAGACTTCTATAAGAAT GGAGACCTTATAGTGGGAGGACCTGGAAGCTTTTACTGGCAAGGTCAGGTGATCACGGTCAGTGTAGCAG ACATCATTGCAAATTACTCATTCAAAGACATTCTTCGAAAACTGGCAGCAGAAAAGCAGACAGATGTGGC TCCAGCTTCCTATGATGACAGCTACCTTGGATATTCAGTTGCTGCTGGAGAATTCACTGGGGACTCTCTG CAAGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAACTTTGGATATGTCTCCATCATTAACTCCACAG ATATGACCTTCATTCAGAATTTTACTGGTGAGCAGATGGCATCTTATTTCGGATATACTGTTGTGGTATC AGATGTTAACAATGATGGCATGGATGACATATTGGTCGGGGCACCTCTCTTTATGGAGCGAGAATTTGAA AGTAACCCTAGAGAAGTGGGTCAGGTCTACCTGTACCTACAAGTGAGCGCCCTCCTCTTCCAAGACCCAC AGGTCCTTACGGGCACAGAGACTTTTGGGAGATTCGGTAGTTCTGTGGCCCACTTGGGGGACCTGAACCA AGATGGATACAATGATATTGCCATCGGTGTGCCCTTTGCAGGCAAGGATCAAAGAGGTAAAGTCCTCATT TACAACGGAAACGCCAGAGGCTTGCACAGCAAGCCTTCCCAAGTTCTGCAAGGAATATGGGGGTCACAGA CCATCCCTTCTGGATTCGGCTTTTCTCTAAGAGGAGACGCAGACATAGACAAGAATGATTACCCAGATTT ACTTGTCGGGGCATTTGGAGAAGGGAAAGTGGCCGTGTACAGAGCAAGGCCAGTTGTAACAGTGGATGCC CAGCTTCTGCTGCACCCAATGATTATCAACCTTGAAAATAAAACTTGCCAGATTCCAGAATTCCCGACTC CTGTGGCCTGCTTTTCTTTAAGAGTCTGTGCATCTATAGCAGGCCAGAGCATTTCAAATACAGTAGCCCT GATGGCCGAGGTGCAGTTAGATTTCCTGAAGCAAAAAGGAGCCATCAAACGGACCCTCTTCCTCCACAAC CACCAGTCCCACCTCATCTTCCCCTTCGTGATGAGGCAGCAAAAATCCCTCCACTGCCAGGATTTTATGG TTTACCTTCGAGATGAAACTGAATTCCGAGATAAACTGTCTCCAATCAACGTTAGCCTGAACTACAGTTT GGATGATTCCACCTTTGAAGATGGCCTGGAAGTGAAGCCGATTTTGAACCACTACAGGGAGAATGTCGTT ACTGAGCAGGCTCACATCCTGGTGGACTGTGGAGAAGACAACCTCTGTGTTCCTGACTTGAGGCTGTCAG CTAGACCAGATAAGCAGGAGATAATTATTGGGGATGAAAATCACCTAATGCTCATAATCAATGCAAGGAA TGAAGGAGAAGGAGCTTACGAAGCTGAACTCTTCGTCATGATTCCCGAAGAAGCAGATTACGTTGGGATC GAGCGCAACAACAAGGGACTGAGGCTCCTGAGTTGTGAGTACAAGATGGAAAACGTAACCAGGATGGTGG TGTGTGACCTTGGGAACCCGATGGTGACTGGAACGAATTTTTCTCTGGGCCTCCGATTTGCTGTTCCTCG TCTTGAGAAAACAAACATGAGTATTAACTTCGATCTCCAAATCAGAAGCTCCAACAAGGACAATCCTGAC AGCAATTTCGTGAGCGTGCAAATCAACGTCACTGCCGTGGCTCAGGTGGAAATCAGAGGAGTGTCCCACC CTCCCCAAATTGTTCTGCCCATCCACAACTGGGAGCCAGCAGAGGAGCCCCACAAAGAGGAGGAAGTCGG TCCCCTGGTTGAACATATTTATGAGCTGCACAATATTGGACCCAGCACCATCAGTGACAGCATTCTCGAA GTGGGCTGGCCATTCTCTGCACGGGAGGAGTTCCTCCTCTATGTCTTTCATCTTCAAACTCTGGGACCTC TACAATGTCAAACAAATCCTGAGATCAACCCGCAGGATATAAAGCCTGCTGCCTCCCCAGAGGATACCCC TGAGCTCAGCGCCTTCTTAAGAAATGCTACAATTCCCCATCTTGTCAGGAAGAGGGATGTGCCAGTGGTC CAGCCCCACAGACAGAGCCCGGCAAAAATACTGAACTGTACCAACATTGACTGCTTGCAAATCTCGTGTG CAGTGGGTCGCCTGGGAGGAGGAGAAAGTGCAGTTCTGAAGGTCAGATCGAGATTGTGGGCGCACACATT CCTCCAGAGAAAGAACGACCCTTATGCTCTTGCATCCCTGGTGTCCTTTGAAGTCAAGAAGATACCTTAT AAAGAGCAGCCAGCAAAACTGCCAGCCGGGAGCACAGCGATTAAGACATCGGTTATTTGGGCCACTCCAA ATGTCTCCTTCTCAATTCCACTGTGGGTCATAATACTAGCGATCCTTCTTGGCTTGCTGGTTCTGGCCAT CTTAACTTTAGCTTTATGGAAGTGTGGATTCTTTGATAGAGCAAGACCTCCTCAGGATGAAATGACAGAC AGGGAACAACTGACCAGTGACAAGACCCCGGAGGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001173972
Insert Size	3189 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001173972.1, NP_001167443.1
RefSeq Size	5896 bp
RefSeq ORF	3189 bp
Locus ID	364786
Gene Summary	acts as a receptor for fibronectin; may be a receptor of matrix ligands and function in glomerular capillary tuft development and physiology [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.