Epha5 (NM_001169137) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EHK-1; Els1; Els1.
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217434 representing NM_001169137 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCTCCGGGCCCCGCGGTGCGGGACGCCGACGGACCCAGGGCAGAGGTGGCGGCGGCGACACCC CCCGCGTCCCTGCCTCTCTGGCAGGCTGCTATTCCGCACCTCTAAAGGGGCCCCTCTGGACGTGCCTTCT CCTGTGTGCGGCGCTCCGGACCCTTTTGGCCAGCCCCAGTAACGAAGTGAATTTATTGGATTCGCGCACT GTCCTGGGAGACCTTGGATGGATTGCTTTTCCAAAGAATGGGTGGGAAGAGATTGGTGAAGTTGATGAAA ACTATGCCCCCATCCACACCTATCAAGTGTGCAAAGTTATGGAACAGAATCAGAATAATTGGCTGTTGAC CAGTTGGATCTCTAACGAAGGTGCTTCCAGAATTTTTATTGAACTCAAGTTTACTCTGAGGGATTGCAAC AGCCTTCCTGGAGGACTGGGGACTTGCAAGGAGACCTTTAACATGTATTATTTTGAGTCGGATGATGAGA ATGGGAGAAATATCAAAGAGAACCAGTACATCAAGATCGATACCATTGCTGCTGATGAGAGCTTCACCGA ACTTGACCTTGGAGACCGGGTCATGAAGCTGAATACGGAGGTCAGAGATGTAGGACCTCTGAGCAAAAAG GGATTTTATCTTGCTTTCCAAGATGTCGGTGCTTGCATCGCTCTGGTTTCTGTCCGTGTCTACTATAAAA AATGTCCTTCTGTAGTTAGACATTTGGCTGTTTTCCCTGACACGATCACTGGAGCAGATTCTTCCCAGTT GCTAGAGGTGTCAGGCTCCTGCGTCAACCATTCTGTGACAGACGATCCTCCCAAAATGCATTGCAGTGCT GAAGGGGAGTGGCTGGTTCCCATCGGGAAATGCATGTGCAAGGCCGGATATGAAGAGAAAAATGGTACCT GTCAAGTGTGCAGACCTGGGTTCTTCAAAGCCTCTCCTCACAGCCAGACCTGCAGCAAATGTCCACCTCA CAGTTACACCCATGAGGAAGCTTCCACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGGGAATCTGAT CCGCCCACAATGGCATGCACAAGACCCCCCTCAGCTCCTCGGAATGCCATCTCAAATGTTAATGAAACTA GTGTCTTTCTGGAGTGGATTCCTCCTGCTGACACTGGTGGACGGAAAGATGTGTCCTATTACATTGCATG CAAGAAGTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTACCTCCCCCAGCAA ATCGGCCTGAAAAATACCTCTGTCATGATGGCGGACCTCCTTGCTCACACAAACTATACCTTTGAAATTG AGGCAGTGAACGGAGTTTCCGACTTGAGTCCAGGCACCCGGCAGTATGTGTCTGTAAATGTAACCACGAA CCAAGCAGCTCCTTCTCCAGTCACCAACGTGAAGAAGGGGAAGATCGCAAAGAACAGCATTTCTTTGTCT TGGCAAGAGCCAGATCGCCCCAATGGGATCATCCTGGAGTACGAAATCAAGTACTTTGAAAAGGACCAAG AGACCAGCTACACAATTATCAAGTCTAAAGAGACCACTATTACGGCAGAGGGCCTGAAACCTGCGTCTGT GTATGTCTTCCAAATTCGAGCACGTACAGCAGCAGGCTACGGCGTCTTCAGTCGACGGTTTGAGTTTGAA ACCACACCAGTGTTTGCAGCATCTAATGATCAAAGCCAGATTCCCATCATTGCGGTGTCAGTGACGGTGG GAGTCATCTTGTTGGCAGTGATGATCGGCTTCCTCCTCAGTGGCAGTTGCTGCGAATGTGGCTGTGGGAG GGCTTCTTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGTGGCTACAGCAAAGCAAAGCAG GATCCAGAAGAGGAAAAGATGCACTTTCATAACGGGCACATTAAACTGCCAGGAGTAAGAACGTACATTG ATCCACACACCTACGAAGATCCCACTCAAGCCGTTCATGAATTTGCCAAGGAGATCGAGGCCTCATGCAT CACCATTGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGTAGTGGACGTTTGAAACTACCCGGA AAAAGAGAATTGCCTGTGGCTATCAAAACTCTTAAAGTAGGCTACACTGAAAAGCAGCGCAGAGATTTTC TGAGTGAAGCGAGTATCATGGGGCAGTTTGATCATCCAAACATCATCCATCTAGAAGGCGTTGTGACTAA AAGTAAACCTGTGATGATAGTGACAGAGTACATGGAGAATGGCTCCTTAGACACATTTTTAAAGAAAAAC GATGGCCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGCATCGCTGCAGGAATGAAGTACCTTT CTGACATGGGCTACGTGCACAGAGACCTTGCTGCTAGAAACATCTTAATCAACAGTAACCTTGTGTGCAA AGTGTCTGACTTTGGACTTTCCAGGGTGCTGGAAGATGATCCTGAGGCAGCCTATACCACAAGGGGAGGC AAAATTCCAATCAGGTGGACTGCTCCAGAAGCAATAGCTTTTCGAAAGTTTACCTCTGCCAGTGATGTCT GGAGCTATGGAATCGTAATGTGGGAAGTTGTGTCCTATGGAGAGAGACCGTACTGGGAGATGACCAATCA GGATGTGATCAAGGCAGTGGAAGAAGGCTACCGCCTGCCAAGCCCCATGGATTGTCCTGCTGCCCTCTAT CAATTAATGCTGGATTGCTGGCAGAAAGATCGCAACAGCAGGCCCAAGTTTGATGACATAGTCAACATGC TGGATAAGCTGATACGAAACCCAAGTAGTTTGAAGACACTGGTGAACGCGTCAAGCAGGGTATCTACTTT GTTGGCAGAACATGGATCTTTGGGGTCTGGGGCCTACAGATCAGTAGGTGAATGGCTAGAAGCAATCAAA ATGGGCCGGTACACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGACCT TGGAGGATTTGAGACGCCTGGGAGTGACTCTGGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGA GATGAAGGTGCAGCTGGTAAACGGGATGGTGCCAGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217434 representing NM_001169137 Red=Cloning site Green=Tags(s) MRGSGPRGAGRRRTQGRGGGGDTPRVPASLAGCYSAPLKGPLWTCLLLCAALRTLLASPSNEVNLLDSRT VLGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCN SLPGGLGTCKETFNMYYFESDDENGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKK GFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDDPPKMHCSA EGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHSQTCSKCPPHSYTHEEASTSCVCEKDYFRRESD PPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPQQ IGLKNTSVMMADLLAHTNYTFEIEAVNGVSDLSPGTRQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLS WQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFE TTPVFAASNDQSQIPIIAVSVTVGVILLAVMIGFLLSGSCCECGCGRASSLCAVAHPSLIWRCGYSKAKQ DPEEEKMHFHNGHIKLPGVRTYIDPHTYEDPTQAVHEFAKEIEASCITIERVIGAGEFGEVCSGRLKLPG KRELPVAIKTLKVGYTEKQRRDFLSEASIMGQFDHPNIIHLEGVVTKSKPVMIVTEYMENGSLDTFLKKN DGQFTVIQLVGMLRGIAAGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGG KIPIRWTAPEAIAFRKFTSASDVWSYGIVMWEVVSYGERPYWEMTNQDVIKAVEEGYRLPSPMDCPAALY QLMLDCWQKDRNSRPKFDDIVNMLDKLIRNPSSLKTLVNASSRVSTLLAEHGSLGSGAYRSVGEWLEAIK MGRYTEIFMENGYSSMDAVAQVTLEDLRRLGVTLVGHQKKIMNSLQEMKVQLVNGMVPV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001169137
ORF Size	3117 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001169137.2, NP_001162608.1
RefSeq Size	8059 bp
RefSeq ORF	3120 bp
Locus ID	79208
MW	115 kDa
Gene Summary	receptor tyrosine kinase; may have a role in both the development and maintenance of differentiated neurons [RGD, Feb 2006]

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