Epha5 (NM_001169137) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	EHK-1; Els1; Els1.
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217434 representing NM_001169137 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCTCCGGGCCCCGCGGTGCGGGACGCCGACGGACCCAGGGCAGAGGTGGCGGCGGCGACACCC CCCGCGTCCCTGCCTCTCTGGCAGGCTGCTATTCCGCACCTCTAAAGGGGCCCCTCTGGACGTGCCTTCT CCTGTGTGCGGCGCTCCGGACCCTTTTGGCCAGCCCCAGTAACGAAGTGAATTTATTGGATTCGCGCACT GTCCTGGGAGACCTTGGATGGATTGCTTTTCCAAAGAATGGGTGGGAAGAGATTGGTGAAGTTGATGAAA ACTATGCCCCCATCCACACCTATCAAGTGTGCAAAGTTATGGAACAGAATCAGAATAATTGGCTGTTGAC CAGTTGGATCTCTAACGAAGGTGCTTCCAGAATTTTTATTGAACTCAAGTTTACTCTGAGGGATTGCAAC AGCCTTCCTGGAGGACTGGGGACTTGCAAGGAGACCTTTAACATGTATTATTTTGAGTCGGATGATGAGA ATGGGAGAAATATCAAAGAGAACCAGTACATCAAGATCGATACCATTGCTGCTGATGAGAGCTTCACCGA ACTTGACCTTGGAGACCGGGTCATGAAGCTGAATACGGAGGTCAGAGATGTAGGACCTCTGAGCAAAAAG GGATTTTATCTTGCTTTCCAAGATGTCGGTGCTTGCATCGCTCTGGTTTCTGTCCGTGTCTACTATAAAA AATGTCCTTCTGTAGTTAGACATTTGGCTGTTTTCCCTGACACGATCACTGGAGCAGATTCTTCCCAGTT GCTAGAGGTGTCAGGCTCCTGCGTCAACCATTCTGTGACAGACGATCCTCCCAAAATGCATTGCAGTGCT GAAGGGGAGTGGCTGGTTCCCATCGGGAAATGCATGTGCAAGGCCGGATATGAAGAGAAAAATGGTACCT GTCAAGTGTGCAGACCTGGGTTCTTCAAAGCCTCTCCTCACAGCCAGACCTGCAGCAAATGTCCACCTCA CAGTTACACCCATGAGGAAGCTTCCACCTCTTGTGTCTGTGAAAAGGATTATTTCAGGAGGGAATCTGAT CCGCCCACAATGGCATGCACAAGACCCCCCTCAGCTCCTCGGAATGCCATCTCAAATGTTAATGAAACTA GTGTCTTTCTGGAGTGGATTCCTCCTGCTGACACTGGTGGACGGAAAGATGTGTCCTATTACATTGCATG CAAGAAGTGCAACTCCCATGCAGGTGTGTGTGAGGAGTGTGGCGGTCATGTCAGGTACCTCCCCCAGCAA ATCGGCCTGAAAAATACCTCTGTCATGATGGCGGACCTCCTTGCTCACACAAACTATACCTTTGAAATTG AGGCAGTGAACGGAGTTTCCGACTTGAGTCCAGGCACCCGGCAGTATGTGTCTGTAAATGTAACCACGAA CCAAGCAGCTCCTTCTCCAGTCACCAACGTGAAGAAGGGGAAGATCGCAAAGAACAGCATTTCTTTGTCT TGGCAAGAGCCAGATCGCCCCAATGGGATCATCCTGGAGTACGAAATCAAGTACTTTGAAAAGGACCAAG AGACCAGCTACACAATTATCAAGTCTAAAGAGACCACTATTACGGCAGAGGGCCTGAAACCTGCGTCTGT GTATGTCTTCCAAATTCGAGCACGTACAGCAGCAGGCTACGGCGTCTTCAGTCGACGGTTTGAGTTTGAA ACCACACCAGTGTTTGCAGCATCTAATGATCAAAGCCAGATTCCCATCATTGCGGTGTCAGTGACGGTGG GAGTCATCTTGTTGGCAGTGATGATCGGCTTCCTCCTCAGTGGCAGTTGCTGCGAATGTGGCTGTGGGAG GGCTTCTTCCCTGTGCGCTGTTGCCCATCCAAGCCTAATATGGCGGTGTGGCTACAGCAAAGCAAAGCAG GATCCAGAAGAGGAAAAGATGCACTTTCATAACGGGCACATTAAACTGCCAGGAGTAAGAACGTACATTG ATCCACACACCTACGAAGATCCCACTCAAGCCGTTCATGAATTTGCCAAGGAGATCGAGGCCTCATGCAT CACCATTGAGAGAGTTATTGGAGCAGGTGAATTTGGTGAAGTTTGTAGTGGACGTTTGAAACTACCCGGA AAAAGAGAATTGCCTGTGGCTATCAAAACTCTTAAAGTAGGCTACACTGAAAAGCAGCGCAGAGATTTTC TGAGTGAAGCGAGTATCATGGGGCAGTTTGATCATCCAAACATCATCCATCTAGAAGGCGTTGTGACTAA AAGTAAACCTGTGATGATAGTGACAGAGTACATGGAGAATGGCTCCTTAGACACATTTTTAAAGAAAAAC GATGGCCAGTTCACTGTGATTCAGCTTGTTGGCATGCTGAGAGGCATCGCTGCAGGAATGAAGTACCTTT CTGACATGGGCTACGTGCACAGAGACCTTGCTGCTAGAAACATCTTAATCAACAGTAACCTTGTGTGCAA AGTGTCTGACTTTGGACTTTCCAGGGTGCTGGAAGATGATCCTGAGGCAGCCTATACCACAAGGGGAGGC AAAATTCCAATCAGGTGGACTGCTCCAGAAGCAATAGCTTTTCGAAAGTTTACCTCTGCCAGTGATGTCT GGAGCTATGGAATCGTAATGTGGGAAGTTGTGTCCTATGGAGAGAGACCGTACTGGGAGATGACCAATCA GGATGTGATCAAGGCAGTGGAAGAAGGCTACCGCCTGCCAAGCCCCATGGATTGTCCTGCTGCCCTCTAT CAATTAATGCTGGATTGCTGGCAGAAAGATCGCAACAGCAGGCCCAAGTTTGATGACATAGTCAACATGC TGGATAAGCTGATACGAAACCCAAGTAGTTTGAAGACACTGGTGAACGCGTCAAGCAGGGTATCTACTTT GTTGGCAGAACATGGATCTTTGGGGTCTGGGGCCTACAGATCAGTAGGTGAATGGCTAGAAGCAATCAAA ATGGGCCGGTACACAGAGATTTTCATGGAAAATGGATACAGTTCAATGGACGCTGTGGCTCAGGTGACCT TGGAGGATTTGAGACGCCTGGGAGTGACTCTGGTCGGTCACCAGAAGAAGATCATGAACAGCCTTCAAGA GATGAAGGTGCAGCTGGTAAACGGGATGGTGCCAGTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001169137
Insert Size	3120 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001169137.2, NP_001162608.1
RefSeq Size	8059 bp
RefSeq ORF	3120 bp
Locus ID	79208
Gene Summary	receptor tyrosine kinase; may have a role in both the development and maintenance of differentiated neurons [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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