Trpc7 (NM_001191691) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217275 representing NM_001191691 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGGGAGCAACACCTTCAAAAACATGCAGCGCCGGCACACCACCTTGAGGGAGAAGGGGCGCCGCC AGGCCATCCGGGGTCCTGCCTACATGTTCAATGAGAAGGGCACCAGCCTGACCCCTGAGGAGGAGCGCTT CCTGGACTCGGCTGAGTATGGCAACATACCAGTGGTCAGGAAAATGTTGGAGGAGTCCAAGACCCTCAAT TTCAACTGCGTGGACTACATGGGGCAGAACGCGCTGCAGCTAGCTGTGGGCAACGAGCACCTAGAGGTCA CTGAACTGCTGCTGAAGAAGGAGAACTTGGCTCGAGTGGGCGACGCGCTGCTGCTGGCCATCAGCAAGGG CTACGTGCGCATTGTGGAGGCCATCCTCAGCCATCCGGCCTTCGCACAGGGCCAGCGGCTGACGCTCAGC CCGCTGGAGCAGGAGCTGCGCGATGACGACTTCTATGCCTACGACGAGGATGGCACGCGCTTCTCCCACG ACATCACACCCATCATCCTGGCGGCCCACTGCCAGGAGTACGAGATTGTACACATCCTGCTGCTCAAGGG TGCGCGCATTGAGCGGCCCCATGACTACTTCTGCAAGTGCAATGAGTGCACCGAGAAGCAGCGCAAGGAC TCCTTCAGCCATTCGCGCTCCCGTATGAATGCCTACAAAGGACTGGCCAGTGCCGCCTACCTGTCCCTAT CCAGTGAAGACCCTGTCCTCACTGCACTGGAGCTAAGCAACGAGTTAGCCAGACTGGCCAACATTGAGAC TGAATTTAAGAACGACTACAGGAAGTTATCTATGCAATGCAAGGACTTTGTGGTGGGCGTGCTGGACCTG TGCCGAGACACGGAAGAGGTGGAAGCAATTTTAAATGGCGACGTGAACTTGCAAGTCTGGTCCGACCACC ACCGTCCAAGTCTGAGCCGCATCAAACTTGCCATTAAGTACGAAGTCAAGAAGTTCGTTGCCCACCCTAA CTGTCAGCAGCAATTGCTTACCATGTGGTATGAAAATCTCTCAGGCTTACGGCAACAGTCTATCGCTGTG AAATTCCTGGCTGTCTTTGGAGTCTCCATAGGCCTCCCTTTTCTCGCCATAGCCTATTGGATTGCTCCGT GCAGCAAGCTAGGACGAACCCTGAGGAGCCCTTTCATGAAGTTCGTAGCCCACGCGGTGTCTTTTACCAT CTTCCTGGGACTGCTAGTTGTGAATGCATCCGACCGGTTTGAGGGTGTTAAGACTTTGCCCAACGAGACC TTCACAGACTACCCCAAACAGATCTTCAGAGTGAAAACCACGCAGTTCTCCTGGACGGAGATGCTCATCA TGAAGTGGGTCTTAGGAATGATCTGGTCTGAATGCAAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGT GCTGCACTTGTGGAACCTACTGGATTTCGGCATGTTGTCCGTCTTCGTGGCCTCCTTCACCGCGAGGTTC ATGGCTTTCCTCAAGGCCAGTGAGGCCCAGCTGTACGTGGACCAGTACGTGCAGGATGTAACGCTGCACA ACGTCTCACTTCCGCCGGAAGTGGCATACTTCACCTACGCCAGGGACAAGTGGTGGCCTTCAGACCCCCA GATCATCTCGGAAGGGCTGTACGCCATCGCTGTTGTACTCAGCTTCTCCCGGATTGCCTATATCCTGCCA GCCAATGAGAGCTTCGGGCCCTTGCAGATCTCCCTGGGGAGAACTGTGAAAGACATCTTCAAGTTCATGG TCATCTTTATCATGGTGTTTGTGGCCTTCATGATCGGGATGTTCAACCTGTACTCCTACTACCGAGGTGC AAAGTACAACCCCGCGTTTACAACAGTTGAAGAGAGCTTTAAAACCCTGTTTTGGTCCATATTTGGCTTG TCTGAGGTCATCTCGGTGGTGCTGAAGTACGACCACAAGTTCATCGAGAACATCGGCTACGTGCTGTACG GCGTTTATAATGTCACCATGGTGGTTGTGCTGCTCAACATGCTGATAGCCATGATCAACAACTCCTATCA GGAAATCGAGGAAGACGCAGACGTGGAGTGGAAATTTGCTCGAGCCAAGCTCTGGCTTTCCTACTTTGAT GAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGTGCCGAGTCCTAAATCCTTTTATTATCTCATAATGA GAATCAAGATGTGCCTCATAAAGCTCTGCAAATCTAAGGCCAAACGCTGTGAAAACGACCTGGAAATGGG CATGCTGAACTCCAAATTCAGGAAGACTCGCTACCAGGCCGGCATGAGGAATTCTGAAAACCTGACAGCT AACAGCACCTTCAGCAAGCCCACCAGATACCAGAAGATCATGAAGCGTCTCATTAAGAGATACGTGCTGA AGGCCCAGGTGGACCGGGAGAATGATGAAGTCAACGAAGGTGAACTCAAAGAGATCAAGCAAGACATCTC AAGCCTTCGCTATGAGCTCCTGGAGGAGAAGTCTCAAGCTACCGGAGAGCTGGCAGACCTGATCCAACAG CTCAGTGAGAAGTTTGGAAAGAATTTGAACAAAGACCACCTGAGGGTGAACAAGGGCAAGGACATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217275 representing NM_001191691 Red=Cloning site Green=Tags(s) MLGSNTFKNMQRRHTTLREKGRRQAIRGPAYMFNEKGTSLTPEEERFLDSAEYGNIPVVRKMLEESKTLN FNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARVGDALLLAISKGYVRIVEAILSHPAFAQGQRLTLS PLEQELRDDDFYAYDEDGTRFSHDITPIILAAHCQEYEIVHILLLKGARIERPHDYFCKCNECTEKQRKD SFSHSRSRMNAYKGLASAAYLSLSSEDPVLTALELSNELARLANIETEFKNDYRKLSMQCKDFVVGVLDL CRDTEEVEAILNGDVNLQVWSDHHRPSLSRIKLAIKYEVKKFVAHPNCQQQLLTMWYENLSGLRQQSIAV KFLAVFGVSIGLPFLAIAYWIAPCSKLGRTLRSPFMKFVAHAVSFTIFLGLLVVNASDRFEGVKTLPNET FTDYPKQIFRVKTTQFSWTEMLIMKWVLGMIWSECKEIWEEGPREYVLHLWNLLDFGMLSVFVASFTARF MAFLKASEAQLYVDQYVQDVTLHNVSLPPEVAYFTYARDKWWPSDPQIISEGLYAIAVVLSFSRIAYILP ANESFGPLQISLGRTVKDIFKFMVIFIMVFVAFMIGMFNLYSYYRGAKYNPAFTTVEESFKTLFWSIFGL SEVISVVLKYDHKFIENIGYVLYGVYNVTMVVVLLNMLIAMINNSYQEIEEDADVEWKFARAKLWLSYFD EGRTLPAPFNLVPSPKSFYYLIMRIKMCLIKLCKSKAKRCENDLEMGMLNSKFRKTRYQAGMRNSENLTA NSTFSKPTRYQKIMKRLIKRYVLKAQVDRENDEVNEGELKEIKQDISSLRYELLEEKSQATGELADLIQQ LSEKFGKNLNKDHLRVNKGKDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191691
ORF Size	2586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191691.2, NP_001178620.2
RefSeq Size	3055 bp
RefSeq ORF	2589 bp
Locus ID	282822
MW	99.5 kDa
Gene Summary	non-selective cation channel; activated down-stream of G-protein-coupled receptors [RGD, Feb 2006]

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