Trpc7 (NM_001191691) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217275 representing NM_001191691 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGGGAGCAACACCTTCAAAAACATGCAGCGCCGGCACACCACCTTGAGGGAGAAGGGGCGCCGCC AGGCCATCCGGGGTCCTGCCTACATGTTCAATGAGAAGGGCACCAGCCTGACCCCTGAGGAGGAGCGCTT CCTGGACTCGGCTGAGTATGGCAACATACCAGTGGTCAGGAAAATGTTGGAGGAGTCCAAGACCCTCAAT TTCAACTGCGTGGACTACATGGGGCAGAACGCGCTGCAGCTAGCTGTGGGCAACGAGCACCTAGAGGTCA CTGAACTGCTGCTGAAGAAGGAGAACTTGGCTCGAGTGGGCGACGCGCTGCTGCTGGCCATCAGCAAGGG CTACGTGCGCATTGTGGAGGCCATCCTCAGCCATCCGGCCTTCGCACAGGGCCAGCGGCTGACGCTCAGC CCGCTGGAGCAGGAGCTGCGCGATGACGACTTCTATGCCTACGACGAGGATGGCACGCGCTTCTCCCACG ACATCACACCCATCATCCTGGCGGCCCACTGCCAGGAGTACGAGATTGTACACATCCTGCTGCTCAAGGG TGCGCGCATTGAGCGGCCCCATGACTACTTCTGCAAGTGCAATGAGTGCACCGAGAAGCAGCGCAAGGAC TCCTTCAGCCATTCGCGCTCCCGTATGAATGCCTACAAAGGACTGGCCAGTGCCGCCTACCTGTCCCTAT CCAGTGAAGACCCTGTCCTCACTGCACTGGAGCTAAGCAACGAGTTAGCCAGACTGGCCAACATTGAGAC TGAATTTAAGAACGACTACAGGAAGTTATCTATGCAATGCAAGGACTTTGTGGTGGGCGTGCTGGACCTG TGCCGAGACACGGAAGAGGTGGAAGCAATTTTAAATGGCGACGTGAACTTGCAAGTCTGGTCCGACCACC ACCGTCCAAGTCTGAGCCGCATCAAACTTGCCATTAAGTACGAAGTCAAGAAGTTCGTTGCCCACCCTAA CTGTCAGCAGCAATTGCTTACCATGTGGTATGAAAATCTCTCAGGCTTACGGCAACAGTCTATCGCTGTG AAATTCCTGGCTGTCTTTGGAGTCTCCATAGGCCTCCCTTTTCTCGCCATAGCCTATTGGATTGCTCCGT GCAGCAAGCTAGGACGAACCCTGAGGAGCCCTTTCATGAAGTTCGTAGCCCACGCGGTGTCTTTTACCAT CTTCCTGGGACTGCTAGTTGTGAATGCATCCGACCGGTTTGAGGGTGTTAAGACTTTGCCCAACGAGACC TTCACAGACTACCCCAAACAGATCTTCAGAGTGAAAACCACGCAGTTCTCCTGGACGGAGATGCTCATCA TGAAGTGGGTCTTAGGAATGATCTGGTCTGAATGCAAGGAGATCTGGGAGGAGGGGCCGCGGGAGTACGT GCTGCACTTGTGGAACCTACTGGATTTCGGCATGTTGTCCGTCTTCGTGGCCTCCTTCACCGCGAGGTTC ATGGCTTTCCTCAAGGCCAGTGAGGCCCAGCTGTACGTGGACCAGTACGTGCAGGATGTAACGCTGCACA ACGTCTCACTTCCGCCGGAAGTGGCATACTTCACCTACGCCAGGGACAAGTGGTGGCCTTCAGACCCCCA GATCATCTCGGAAGGGCTGTACGCCATCGCTGTTGTACTCAGCTTCTCCCGGATTGCCTATATCCTGCCA GCCAATGAGAGCTTCGGGCCCTTGCAGATCTCCCTGGGGAGAACTGTGAAAGACATCTTCAAGTTCATGG TCATCTTTATCATGGTGTTTGTGGCCTTCATGATCGGGATGTTCAACCTGTACTCCTACTACCGAGGTGC AAAGTACAACCCCGCGTTTACAACAGTTGAAGAGAGCTTTAAAACCCTGTTTTGGTCCATATTTGGCTTG TCTGAGGTCATCTCGGTGGTGCTGAAGTACGACCACAAGTTCATCGAGAACATCGGCTACGTGCTGTACG GCGTTTATAATGTCACCATGGTGGTTGTGCTGCTCAACATGCTGATAGCCATGATCAACAACTCCTATCA GGAAATCGAGGAAGACGCAGACGTGGAGTGGAAATTTGCTCGAGCCAAGCTCTGGCTTTCCTACTTTGAT GAAGGAAGAACTCTCCCTGCTCCCTTTAACCTGGTGCCGAGTCCTAAATCCTTTTATTATCTCATAATGA GAATCAAGATGTGCCTCATAAAGCTCTGCAAATCTAAGGCCAAACGCTGTGAAAACGACCTGGAAATGGG CATGCTGAACTCCAAATTCAGGAAGACTCGCTACCAGGCCGGCATGAGGAATTCTGAAAACCTGACAGCT AACAGCACCTTCAGCAAGCCCACCAGATACCAGAAGATCATGAAGCGTCTCATTAAGAGATACGTGCTGA AGGCCCAGGTGGACCGGGAGAATGATGAAGTCAACGAAGGTGAACTCAAAGAGATCAAGCAAGACATCTC AAGCCTTCGCTATGAGCTCCTGGAGGAGAAGTCTCAAGCTACCGGAGAGCTGGCAGACCTGATCCAACAG CTCAGTGAGAAGTTTGGAAAGAATTTGAACAAAGACCACCTGAGGGTGAACAAGGGCAAGGACATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191691
Insert Size	2589 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191691.2, NP_001178620.2
RefSeq Size	3055 bp
RefSeq ORF	2589 bp
Locus ID	282822
Gene Summary	non-selective cation channel; activated down-stream of G-protein-coupled receptors [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.