Parpbp (NM_001166676) Rat Tagged ORF Clone
CAT#: RR216782
- TrueORF®
Parpbp (myc-DDK-tagged) - Rat PARP1 binding protein (Parpbp)
"NM_001166676" in other vectors (1)
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CNY 4,660.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AROM; PARI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR216782 representing NM_001166676
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGGTCGACCACTATGAAGACATTAGGAAAACATACGATGATTTCTTGAAGAATAGCAATACAC TAGATCTGATTGATGTTTATAAAAAATGTAGCATCATGACTTCTAATTGTGAGAAACACATGATATCCTC TATTCAACTACGGGACTTTCTATCTGGCACAGAGTGTGCAGTAAATGATGAAAATAATCCTCATAGCCCA GCGTCACCAGTGAAATGCAGCCAGAATGACAAACAGGTGCGGCTGTTGGCAAAGAAAATTTTCCTTTCGT ATTTGAGCCTCTTAGTGAATTCAAAGAATGACCTGGCTCTGGCTCAAATTCTCAACACCCCTGACCGAGG ACTGGGAAGGGAGGCCTTCACCAACTTGAAGCATGCTGCCCGAGAGAAACAGTTGTCTATGTTTCTGATG GCCACATCATTTATTAGGACGTTAGAGCTCGGAGGGAAAGGATATGCACCCCCACCATCAGATCCTCTGT GGGCACATGTAAAAGGGCTGTCTGCTTTTGTTAATTTCATTGACAAGCTAAATGAAATTCTTGGAGAGGT ACCAAATCCAAGCCTTGCGGGCGGTCGGATATTATCGGCGATAAGGACGCAGCTGATCAAAGGCCACAGC AGCGGGGAGCCTTTTTATAAAGCAGTAGAGGAAGTTGTTCAGGATTTGAATTTGAGGATTAAAAATATTA TTAATTCTCAAGAAGGTGTAACTGTCAGCGCCACCAACATCAGTCCTGTCCCGCCAAAATCTTTTGCCAT AAACCATGATACCGCATACTGTGGAAGAGATGCTGTGAAAATTTTACTGGTTCTTTTGGATGAAGACGCA GCCAGTGCTCCTACCAGAAATAAAGCAGAGCTTCTGTACAATGACCAAAGTACAGCTCCTCACGGTGGAG CGTCTGTCCTTACACTCTTTAGGTCTCCCACACAAGTGAAAAGTACACCAGTTAAACCTCTCAGAGAGCG AATCCACACGTCACTGCAAGCAGAGAAAAATAAGCAGATGAGGCAGACATTAATTAGATCACAGTTTGCT TGTACTTATAAAGATGACTGCATAATGAGCAAGAGTAAATGGAATGTTAATTCCTCATCAAAGCCGCTGT CTGCTCTTCGCTTAGAGAACGATGTCTCTGAGGGAGCACAGCCGTCTGTTGGGAAAGCCAGACTTGAAAC GAGTTCTGAAAATGTGCATGTGGACAGAAGTAAGGATGACAAAGGACCGAGGAAGTCTACTAAACGGAAG CTAGCGAAGAGCAAGCAGCCGGGTGTCAGGGAGGATATTCACTGCGATCAGGGAGATGAGCTGTACCAAC CCAAGAATGTTAAGATCCTTAAAGTGCCCAGTGACTCTCACAGGAAAGCTGGTGGCAAACTAGCCCCAGG AGCAAGGGGCAACAGGTGCACCACCAAGGACAAGCTGATCCCTGGCCAGACCAAGCTGACACGGTTTTTT ATGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR216782 representing NM_001166676
Red=Cloning site Green=Tags(s) MKVVDHYEDIRKTYDDFLKNSNTLDLIDVYKKCSIMTSNCEKHMISSIQLRDFLSGTECAVNDENNPHSP ASPVKCSQNDKQVRLLAKKIFLSYLSLLVNSKNDLALAQILNTPDRGLGREAFTNLKHAAREKQLSMFLM ATSFIRTLELGGKGYAPPPSDPLWAHVKGLSAFVNFIDKLNEILGEVPNPSLAGGRILSAIRTQLIKGHS SGEPFYKAVEEVVQDLNLRIKNIINSQEGVTVSATNISPVPPKSFAINHDTAYCGRDAVKILLVLLDEDA ASAPTRNKAELLYNDQSTAPHGGASVLTLFRSPTQVKSTPVKPLRERIHTSLQAEKNKQMRQTLIRSQFA CTYKDDCIMSKSKWNVNSSSKPLSALRLENDVSEGAQPSVGKARLETSSENVHVDRSKDDKGPRKSTKRK LAKSKQPGVREDIHCDQGDELYQPKNVKILKVPSDSHRKAGGKLAPGARGNRCTTKDKLIPGQTKLTRFF ML TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001166676 |
ORF Size | 1476 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001166676.1, NP_001160148.1 |
RefSeq Size | 4427 bp |
RefSeq ORF | 1479 bp |
Locus ID | 100310874 |
UniProt ID | Q9EQ10 |
MW | 54.5 kDa |
Gene Summary | Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect (By similarity). Binds single-strand DNA and poly(A) homopolymers.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN216782 | Parpbp (untagged) - Rat PARP1 binding protein (Parpbp) |
CNY 4,660.00 |