Parpbp (NM_001166676) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AROM; PARI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216782 representing NM_001166676 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGGTCGACCACTATGAAGACATTAGGAAAACATACGATGATTTCTTGAAGAATAGCAATACAC TAGATCTGATTGATGTTTATAAAAAATGTAGCATCATGACTTCTAATTGTGAGAAACACATGATATCCTC TATTCAACTACGGGACTTTCTATCTGGCACAGAGTGTGCAGTAAATGATGAAAATAATCCTCATAGCCCA GCGTCACCAGTGAAATGCAGCCAGAATGACAAACAGGTGCGGCTGTTGGCAAAGAAAATTTTCCTTTCGT ATTTGAGCCTCTTAGTGAATTCAAAGAATGACCTGGCTCTGGCTCAAATTCTCAACACCCCTGACCGAGG ACTGGGAAGGGAGGCCTTCACCAACTTGAAGCATGCTGCCCGAGAGAAACAGTTGTCTATGTTTCTGATG GCCACATCATTTATTAGGACGTTAGAGCTCGGAGGGAAAGGATATGCACCCCCACCATCAGATCCTCTGT GGGCACATGTAAAAGGGCTGTCTGCTTTTGTTAATTTCATTGACAAGCTAAATGAAATTCTTGGAGAGGT ACCAAATCCAAGCCTTGCGGGCGGTCGGATATTATCGGCGATAAGGACGCAGCTGATCAAAGGCCACAGC AGCGGGGAGCCTTTTTATAAAGCAGTAGAGGAAGTTGTTCAGGATTTGAATTTGAGGATTAAAAATATTA TTAATTCTCAAGAAGGTGTAACTGTCAGCGCCACCAACATCAGTCCTGTCCCGCCAAAATCTTTTGCCAT AAACCATGATACCGCATACTGTGGAAGAGATGCTGTGAAAATTTTACTGGTTCTTTTGGATGAAGACGCA GCCAGTGCTCCTACCAGAAATAAAGCAGAGCTTCTGTACAATGACCAAAGTACAGCTCCTCACGGTGGAG CGTCTGTCCTTACACTCTTTAGGTCTCCCACACAAGTGAAAAGTACACCAGTTAAACCTCTCAGAGAGCG AATCCACACGTCACTGCAAGCAGAGAAAAATAAGCAGATGAGGCAGACATTAATTAGATCACAGTTTGCT TGTACTTATAAAGATGACTGCATAATGAGCAAGAGTAAATGGAATGTTAATTCCTCATCAAAGCCGCTGT CTGCTCTTCGCTTAGAGAACGATGTCTCTGAGGGAGCACAGCCGTCTGTTGGGAAAGCCAGACTTGAAAC GAGTTCTGAAAATGTGCATGTGGACAGAAGTAAGGATGACAAAGGACCGAGGAAGTCTACTAAACGGAAG CTAGCGAAGAGCAAGCAGCCGGGTGTCAGGGAGGATATTCACTGCGATCAGGGAGATGAGCTGTACCAAC CCAAGAATGTTAAGATCCTTAAAGTGCCCAGTGACTCTCACAGGAAAGCTGGTGGCAAACTAGCCCCAGG AGCAAGGGGCAACAGGTGCACCACCAAGGACAAGCTGATCCCTGGCCAGACCAAGCTGACACGGTTTTTT ATGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216782 representing NM_001166676 Red=Cloning site Green=Tags(s) MKVVDHYEDIRKTYDDFLKNSNTLDLIDVYKKCSIMTSNCEKHMISSIQLRDFLSGTECAVNDENNPHSP ASPVKCSQNDKQVRLLAKKIFLSYLSLLVNSKNDLALAQILNTPDRGLGREAFTNLKHAAREKQLSMFLM ATSFIRTLELGGKGYAPPPSDPLWAHVKGLSAFVNFIDKLNEILGEVPNPSLAGGRILSAIRTQLIKGHS SGEPFYKAVEEVVQDLNLRIKNIINSQEGVTVSATNISPVPPKSFAINHDTAYCGRDAVKILLVLLDEDA ASAPTRNKAELLYNDQSTAPHGGASVLTLFRSPTQVKSTPVKPLRERIHTSLQAEKNKQMRQTLIRSQFA CTYKDDCIMSKSKWNVNSSSKPLSALRLENDVSEGAQPSVGKARLETSSENVHVDRSKDDKGPRKSTKRK LAKSKQPGVREDIHCDQGDELYQPKNVKILKVPSDSHRKAGGKLAPGARGNRCTTKDKLIPGQTKLTRFF ML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001166676
ORF Size	1476 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001166676.1, NP_001160148.1
RefSeq Size	4427 bp
RefSeq ORF	1479 bp
Locus ID	100310874
UniProt ID	Q9EQ10
MW	54.5 kDa
Gene Summary	Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect (By similarity). Binds single-strand DNA and poly(A) homopolymers.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.