Parpbp (NM_001166676) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AROM; PARI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN216782 representing NM_001166676 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGGTCGACCACTATGAAGACATTAGGAAAACATACGATGATTTCTTGAAGAATAGCAATACAC TAGATCTGATTGATGTTTATAAAAAATGTAGCATCATGACTTCTAATTGTGAGAAACACATGATATCCTC TATTCAACTACGGGACTTTCTATCTGGCACAGAGTGTGCAGTAAATGATGAAAATAATCCTCATAGCCCA GCGTCACCAGTGAAATGCAGCCAGAATGACAAACAGGTGCGGCTGTTGGCAAAGAAAATTTTCCTTTCGT ATTTGAGCCTCTTAGTGAATTCAAAGAATGACCTGGCTCTGGCTCAAATTCTCAACACCCCTGACCGAGG ACTGGGAAGGGAGGCCTTCACCAACTTGAAGCATGCTGCCCGAGAGAAACAGTTGTCTATGTTTCTGATG GCCACATCATTTATTAGGACGTTAGAGCTCGGAGGGAAAGGATATGCACCCCCACCATCAGATCCTCTGT GGGCACATGTAAAAGGGCTGTCTGCTTTTGTTAATTTCATTGACAAGCTAAATGAAATTCTTGGAGAGGT ACCAAATCCAAGCCTTGCGGGCGGTCGGATATTATCGGCGATAAGGACGCAGCTGATCAAAGGCCACAGC AGCGGGGAGCCTTTTTATAAAGCAGTAGAGGAAGTTGTTCAGGATTTGAATTTGAGGATTAAAAATATTA TTAATTCTCAAGAAGGTGTAACTGTCAGCGCCACCAACATCAGTCCTGTCCCGCCAAAATCTTTTGCCAT AAACCATGATACCGCATACTGTGGAAGAGATGCTGTGAAAATTTTACTGGTTCTTTTGGATGAAGACGCA GCCAGTGCTCCTACCAGAAATAAAGCAGAGCTTCTGTACAATGACCAAAGTACAGCTCCTCACGGTGGAG CGTCTGTCCTTACACTCTTTAGGTCTCCCACACAAGTGAAAAGTACACCAGTTAAACCTCTCAGAGAGCG AATCCACACGTCACTGCAAGCAGAGAAAAATAAGCAGATGAGGCAGACATTAATTAGATCACAGTTTGCT TGTACTTATAAAGATGACTGCATAATGAGCAAGAGTAAATGGAATGTTAATTCCTCATCAAAGCCGCTGT CTGCTCTTCGCTTAGAGAACGATGTCTCTGAGGGAGCACAGCCGTCTGTTGGGAAAGCCAGACTTGAAAC GAGTTCTGAAAATGTGCATGTGGACAGAAGTAAGGATGACAAAGGACCGAGGAAGTCTACTAAACGGAAG CTAGCGAAGAGCAAGCAGCCGGGTGTCAGGGAGGATATTCACTGCGATCAGGGAGATGAGCTGTACCAAC CCAAGAATGTTAAGATCCTTAAAGTGCCCAGTGACTCTCACAGGAAAGCTGGTGGCAAACTAGCCCCAGG AGCAAGGGGCAACAGGTGCACCACCAAGGACAAGCTGATCCCTGGCCAGACCAAGCTGACACGGTTTTTT ATGCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001166676
Insert Size	1479 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001166676.1, NP_001160148.1
RefSeq Size	4427 bp
RefSeq ORF	1479 bp
Locus ID	100310874
UniProt ID	Q9EQ10
Gene Summary	Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect (By similarity). Binds single-strand DNA and poly(A) homopolymers.[UniProtKB/Swiss-Prot Function]

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